FastQCFastQC Report
Wed 25 May 2016
SRR1780138_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780138_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69272
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1095915.82024483196674No Hit
CTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGT34114.92406744427763No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCG11861.7120914655271973No Hit
ATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGTCTT8291.196731724217577RNA PCR Primer, Index 41 (95% over 22bp)
CTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGTC5640.8141817761866267No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCC5630.8127381914770758No Hit
ATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGTCAT5100.7362282018708858No Hit
CTTTACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGTC3960.5716595449820995No Hit
CTTAATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCG2880.41575239635061784No Hit
ATTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGT2450.35367825383993534No Hit
CTTATACACATCTCCGAGCCCACGAGACAAAAAGACATCTCGTATGCCGT2370.34212957616352924No Hit
CTTATACACATCTCCGAGCCCACGAGACAATAAGACATCACGTATGCCGT2330.33635523732532624No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCC2090.3017092042961081No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTAGTATGCCG1980.28582977249104974No Hit
CTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGACGT1770.2555144935904839No Hit
TTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGTC1650.23819147707587482No Hit
TATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGTCT1500.21653770643261347RNA PCR Primer, Index 41 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGA1440.20787619817530892No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGAC1300.18766601224159835No Hit
CTTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCG1290.1862224275320476No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGACG1180.17034299572698927No Hit
CTTATACACATCTCCGAGCCCACGAGACAATAAGAAATCTCGTATGCCGT1150.166012241598337No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATTCCG910.13136620856911885No Hit
CATATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGT860.12414828502136505No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATTCC780.112599607344959No Hit
CTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTAGTATGCCGT770.11115602263540826No Hit
TCTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGT750.10826885321630673No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCGTC150.002216061870.02
AGCCGAC150.002216061870.045
CGAAATA207.876203E-570.048
CGTCTAC252.8128816E-670.048
TCCTATG150.002216061870.040
TGACGAA150.002216061870.045
TGTGCCC150.002216061870.018
AATGCCG207.876203E-570.043
CGAATGA150.002216061870.041
AAAGCCG150.002216061870.043
GCCGACA401.047556E-861.2546
TCTCGAA308.297577E-658.33333238
CCGACAT308.297577E-658.33333247
GCCGAAT252.3753263E-455.99999647
TCCGTCA252.3753263E-455.99999648
CCGAATT252.3753263E-455.99999648
CTCCTAT252.3753263E-455.99999639
ACGAATG252.3753263E-455.99999640
AGCGTCA200.006924618552.53
CGGTTGT200.006924618552.525
Produced by FastQC (version 0.11.5)