##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780138_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 69272 Sequences flagged as poor quality 0 Sequence length 76 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.704902413673633 32.0 27.0 32.0 13.0 32.0 2 27.512458136043424 32.0 27.0 32.0 13.0 32.0 3 28.21201928629172 32.0 27.0 32.0 13.0 32.0 4 29.93116988104862 32.0 32.0 32.0 27.0 32.0 5 29.114505139161565 32.0 27.0 32.0 22.0 32.0 6 32.226787157870426 37.0 32.0 37.0 13.0 37.0 7 33.66061323478462 37.0 37.0 37.0 22.0 37.0 8 32.93160295646148 37.0 32.0 37.0 22.0 37.0 9 33.86551564845825 37.0 37.0 37.0 27.0 37.0 10-11 33.636021769257425 37.0 34.5 37.0 22.0 37.0 12-13 33.667109365977595 37.0 34.5 37.0 24.5 37.0 14-15 33.6995611502483 37.0 34.5 37.0 22.0 37.0 16-17 32.68300323362975 37.0 32.0 37.0 17.5 37.0 18-19 32.9130889825615 37.0 32.0 37.0 17.5 37.0 20-21 33.49762530315279 37.0 34.5 37.0 22.0 37.0 22-23 33.07300207876199 37.0 32.0 37.0 22.0 37.0 24-25 33.03616179697424 37.0 32.0 37.0 17.5 37.0 26-27 33.18202881395081 37.0 32.0 37.0 17.5 37.0 28-29 33.2032639450283 37.0 32.0 37.0 22.0 37.0 30-31 32.79052864072064 37.0 32.0 37.0 17.5 37.0 32-33 32.65611646841437 37.0 32.0 37.0 13.0 37.0 34-35 32.05790940062363 37.0 32.0 37.0 13.0 37.0 36-37 32.02715382838665 37.0 32.0 37.0 13.0 37.0 38-39 30.59000750664049 37.0 27.0 37.0 13.0 37.0 40-41 29.273378854371174 37.0 24.5 37.0 10.5 37.0 42-43 29.137768506755975 37.0 24.5 37.0 10.5 37.0 44-45 30.499191592562653 37.0 27.0 37.0 13.0 37.0 46-47 28.74321515186511 37.0 22.0 37.0 10.5 37.0 48-49 28.200145802055665 34.5 17.5 37.0 10.5 37.0 50-51 27.64001328097933 32.0 13.0 37.0 8.0 37.0 52-53 27.80166589675482 32.0 13.0 37.0 13.0 37.0 54-55 27.56986228201871 32.0 13.0 37.0 13.0 37.0 56-57 26.95176983485391 32.0 13.0 37.0 8.0 37.0 58-59 28.187644358470955 32.0 17.5 37.0 13.0 37.0 60-61 28.139515244254532 34.5 17.5 37.0 10.5 37.0 62-63 30.051961831620282 37.0 27.0 37.0 13.0 37.0 64-65 30.522440524309967 37.0 27.0 37.0 13.0 37.0 66-67 30.214798186857607 37.0 27.0 37.0 13.0 37.0 68-69 29.189419967663703 37.0 22.0 37.0 13.0 37.0 70-71 27.457544173692114 32.0 13.0 37.0 13.0 37.0 72-73 26.347716248989492 32.0 13.0 37.0 8.0 37.0 74-75 26.811937001963276 32.0 13.0 37.0 8.0 37.0 76 27.124220464256844 32.0 13.0 37.0 8.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 11101 1 -2.9254004249231613 11101 2 -4.438940473221972 11101 3 1.887841784228094 11101 4 0.7250878787244837 11101 5 -1.2339886960866444 11101 6 -0.4034420278894508 11101 7 0.4248085406842961 11101 8 -1.81286118753939 11101 9 -1.060028253125033 11101 10-11 -0.8362312455860348 11101 12-13 -1.7196738303883912 11101 14-15 -1.2265135582346858 11101 16-17 -1.052412092104916 11101 18-19 0.7135085187153862 11101 20-21 -1.1337216133282908 11101 22-23 0.7650319581083025 11101 24-25 0.15809857226660284 11101 26-27 -1.314529673824687 11101 28-29 -1.9115496055069272 11101 30-31 -1.2406959338891852 11101 32-33 -0.15593560068722212 11101 34-35 -0.3861683148610311 11101 36-37 -0.8157516158696332 11101 38-39 -0.6983392848762122 11101 40-41 1.3296209238240806 11101 42-43 0.806118496184375 11101 44-45 -0.7502941654266344 11101 46-47 -0.02697473820390428 11101 48-49 -0.5904257627302059 11101 50-51 3.0864317248424165 11101 52-53 1.8326400012326793 11101 54-55 3.655013060860405 11101 56-57 1.4397588103032604 11101 58-59 0.45627165656234325 11101 60-61 -0.8961290431484841 11101 62-63 -0.8506493054384059 11101 64-65 -3.0349943067410408 11101 66-67 -1.9179348224117732 11101 68-69 -2.74614052569952 11101 70-71 -2.7288488738323338 11101 72-73 1.8257159092064192 11101 74-75 0.7918232097742894 11101 76 -0.20790662155591733 11102 1 -0.6208463452267665 11102 2 -0.9569670386868694 11102 3 0.8052990897309407 11102 4 -0.382122747461473 11102 5 0.19485380865718938 11102 6 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11104 26-27 1.054694388947766 11104 28-29 0.5413710570911547 11104 30-31 1.0051628280986051 11104 32-33 -0.036929497810149314 11104 34-35 0.18445069124184243 11104 36-37 -0.43955283644504917 11104 38-39 0.1874497299450617 11104 40-41 -1.1743023542927453 11104 42-43 1.3309659242576117 11104 44-45 -1.146545255226112 11104 46-47 2.1957802748736874 11104 48-49 1.355956103006502 11104 50-51 0.21807950165148426 11104 52-53 0.9608004197156781 11104 54-55 1.301918030346016 11104 56-57 -0.5794216604901159 11104 58-59 -2.058985536114207 11104 60-61 1.1513862140441944 11104 62-63 -1.14663884336343 11104 64-65 -1.1483334523382496 11104 66-67 0.8972351863066237 11104 68-69 1.796143694004055 11104 70-71 -1.2436701467181237 11104 72-73 -0.14028234711441812 11104 74-75 -0.8550817597113252 11104 76 0.13298265481722993 11105 1 0.24843470052486794 11105 2 -1.0321304373796778 11105 3 1.1190245799270215 11105 4 -0.23996588471637637 11105 5 -0.40155142010097933 11105 6 0.9182425599241704 11105 7 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0.5562788291391918 11105 70-71 1.457531054483077 11105 72-73 0.4242822174501484 11105 74-75 2.0803536757241083 11105 76 3.810910582745155 11106 1 0.9164563807416712 11106 2 -0.9684448005233115 11106 3 -0.9013006233250138 11106 4 0.17670078195029149 11106 5 0.22785237393696178 11106 6 -0.4726788492899239 11106 7 0.5860988632649438 11106 8 0.49634416257863023 11106 9 -1.5918929266104769 11106 10-11 -0.5455152739101976 11106 12-13 -0.9443001089092391 11106 14-15 -0.5483074213346129 11106 16-17 -1.4265269622858767 11106 18-19 -1.3384190973349632 11106 20-21 1.0275687092523498 11106 22-23 -0.28964939515684307 11106 24-25 -0.373372710188157 11106 26-27 0.061945542540385645 11106 28-29 -0.07992096663988946 11106 30-31 -0.8952986089481954 11106 32-33 0.5962280499815407 11106 34-35 0.20785214147256426 11106 36-37 0.8313766296063676 11106 38-39 0.17459541221269248 11106 40-41 -0.5787189660264289 11106 42-43 -0.680899599803034 11106 44-45 -0.7487206013038978 11106 46-47 -1.3342131331685003 11106 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-0.4041935592775161 21612 62-63 0.859028113916434 21612 64-65 0.2633927900331514 21612 66-67 -0.6840638546698372 21612 68-69 0.6490207646230637 21612 70-71 -0.34981661576781775 21612 72-73 -1.3597679617613245 21612 74-75 -0.3775316289353903 21612 76 -2.465971137684953 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 7.0 17 21.0 18 51.0 19 138.0 20 330.0 21 630.0 22 1158.0 23 1745.0 24 2336.0 25 2898.0 26 3618.0 27 4471.0 28 5351.0 29 6286.0 30 7017.0 31 7681.0 32 7113.0 33 6016.0 34 4417.0 35 3785.0 36 4203.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.751407535729754 26.21914248592464 15.326981377219576 22.70246860112603 2 28.244142570484044 20.66376983153123 32.42049342437672 18.671594173608003 3 29.466876470673153 25.62399849865023 33.871317001342554 11.037808029334064 4 25.544231435500635 26.918524078992956 32.64811179119991 14.8891326943065 5 25.821399699734382 36.300381106363325 32.67265273126227 5.205566462640028 6 31.952244149619595 39.52850481456351 16.47778948736087 12.041461548456018 7 28.848596835662317 31.683797205220003 15.647014666820649 23.82059129229703 8 28.05029449128075 39.7779766716711 14.303037302228894 17.868691534819263 9 28.47903915001732 20.474361935558377 13.158274627555144 37.88832428686915 10-11 29.09472802864072 28.4826481117912 24.97473726758286 17.447886591985217 12-13 30.11389883358355 17.070389190437695 27.52338607229472 25.292325903684027 14-15 31.149670862686225 17.95530661739231 17.407466220117797 33.48755629980367 16-17 39.780863841090195 26.503493474997114 13.880066982330524 19.83557570158217 18-19 39.41821856503537 23.043886242240507 14.211058178143496 23.326837014580626 20-21 30.68339300150133 18.896523848019402 13.641875505254648 36.77820764522462 22-23 30.760347042774033 29.69207893635143 13.676718973307732 25.870855047566803 24-25 39.7311987065201 29.285915360795723 14.130113107311193 16.852772825372995 26-27 39.7611759269955 33.99561048862193 14.022611759269953 12.220601825112626 28-29 28.711270249198694 33.89593139095031 16.402211891080245 20.990586468770754 30-31 28.976489356326653 36.165390195837816 22.66607956227036 12.192040885565172 32-33 28.845431970431974 40.09225571725572 19.095056595056594 11.967255717255718 34-35 37.712301773482224 33.2212124930526 16.58570387105622 12.48078186240896 36-37 28.708569118835893 31.11719020672133 17.69907033144705 22.475170342995725 38-39 28.425626515763945 22.637573622820188 26.1931227624437 22.743677098972167 40-41 28.872832787169234 20.927227844263832 26.886431551442886 23.313507817124048 42-43 38.63232810266923 19.732644252284505 26.820747498953384 14.814280146092882 44-45 30.15864995957963 28.24950918119875 27.789005658852062 13.802835200369557 46-47 38.01862278042443 19.883066262451276 21.65728309513498 20.441027861989316 48-49 36.0607460445779 18.78464603302922 17.982734726873773 27.171873195519115 50-51 30.448088693844554 20.664193324864303 27.23250375332025 21.655214227970898 52-53 27.549370597066634 23.08725025984525 33.6362455248874 15.727133618200718 54-55 27.89871809677792 24.510624783462294 27.313344497055088 20.2773126227047 56-57 36.982474881626054 21.669650075066404 20.438994110174384 20.908880933133155 58-59 29.01626209913168 26.059779274304727 29.93222320867889 14.9917354178847 60-61 35.67891788890172 33.34825037533202 19.741020903106595 11.231810832659662 62-63 27.24155851655094 47.11928512653203 14.414401408959016 11.22475494795802 64-65 25.630864732207304 49.03493575862567 14.292623069149704 11.041576440017325 66-67 25.676338963476255 49.17063663923776 14.284683124007508 10.868341273278476 68-69 28.22352465642684 46.63283866497286 14.173836470724103 10.9698002078762 70-71 33.46229356738654 41.18908072525696 13.981839704353852 11.366786003002655 72-73 50.14508235769658 24.11687430526483 14.431724675549654 11.306318661488934 74-75 55.25753551218385 19.350531239173115 14.095161104053586 11.296772144589443 76 56.50064963187526 18.673307348058323 13.71300707376931 11.113035946297098 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 1.0 5 2.0 6 2.5 7 2.5 8 3.0 9 5.5 10 13.0 11 20.0 12 22.0 13 35.0 14 59.0 15 83.0 16 108.0 17 120.0 18 157.5 19 237.0 20 329.5 21 380.0 22 415.0 23 498.0 24 633.5 25 721.0 26 805.5 27 936.0 28 1062.0 29 1142.0 30 1219.5 31 1412.0 32 1659.0 33 1791.0 34 1906.0 35 2087.5 36 2267.0 37 2380.0 38 2539.0 39 2806.5 40 3222.5 41 3750.0 42 3970.0 43 4480.0 44 5234.0 45 4812.0 46 4146.0 47 3425.5 48 2516.5 49 1759.0 50 1190.0 51 1113.0 52 948.5 53 815.0 54 769.0 55 746.5 56 674.5 57 585.5 58 546.0 59 502.0 60 418.0 61 336.5 62 295.0 63 251.5 64 197.0 65 169.0 66 132.5 67 113.0 68 109.5 69 90.0 70 70.0 71 66.0 72 57.5 73 38.0 74 23.0 75 19.0 76 20.0 77 16.5 78 11.0 79 10.0 80 10.0 81 8.0 82 7.5 83 9.0 84 9.5 85 10.0 86 7.5 87 5.0 88 5.5 89 5.5 90 8.0 91 13.0 92 15.0 93 17.5 94 29.5 95 60.5 96 82.0 97 109.5 98 276.0 99 5538.0 100 10661.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.002887169419101513 2 0.0014435847095507564 3 0.0014435847095507564 4 0.0 5 0.0 6 0.004330754128652269 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.002887169419101513 20-21 0.0 22-23 0.0014435847095507564 24-25 0.0021653770643261347 26-27 0.023097355352812102 28-29 0.014435847095507564 30-31 0.00793971590252916 32-33 0.011548677676406051 34-35 0.0021653770643261347 36-37 0.0 38-39 0.0 40-41 0.0014435847095507564 42-43 0.0014435847095507564 44-45 0.0 46-47 0.002887169419101513 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 7.217923547753782E-4 60-61 0.0 62-63 0.0014435847095507564 64-65 0.002887169419101513 66-67 0.002887169419101513 68-69 0.0 70-71 0.0 72-73 0.0014435847095507564 74-75 0.0 76 0.002887169419101513 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 69272.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.128883242868696 #Duplication Level Percentage of deduplicated Percentage of total 1 82.84488113388673 42.3576625476383 2 10.37043311310633 10.604573276359856 3 3.7523293240725057 5.755572236978866 4 1.4032412897396804 2.869846402586904 5 0.6126828166469027 1.5662894098625708 6 0.2569315037551527 0.7881972514147131 7 0.16093511773674404 0.5759902991107518 8 0.053645039245581345 0.219424875851715 9 0.07623242419108928 0.35079108442083384 >10 0.3783386978372579 3.6522693151634136 >50 0.028234231181884915 1.007622127266428 >100 0.042351346772827377 4.23547753782192 >500 0.011293692472753965 3.559879893752165 >1k 0.005646846236376983 6.636158909804828 >5k 0.0 0.0 >10k+ 0.0028234231181884914 15.82024483196674 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 10959 15.82024483196674 No Hit CTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGT 3411 4.92406744427763 No Hit TCTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCG 1186 1.7120914655271973 No Hit ATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGTCTT 829 1.196731724217577 RNA PCR Primer, Index 41 (95% over 22bp) CTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGTC 564 0.8141817761866267 No Hit TCTTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCC 563 0.8127381914770758 No Hit ATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGTCAT 510 0.7362282018708858 No Hit CTTTACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGTC 396 0.5716595449820995 No Hit CTTAATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCG 288 0.41575239635061784 No Hit ATTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGT 245 0.35367825383993534 No Hit CTTATACACATCTCCGAGCCCACGAGACAAAAAGACATCTCGTATGCCGT 237 0.34212957616352924 No Hit CTTATACACATCTCCGAGCCCACGAGACAATAAGACATCACGTATGCCGT 233 0.33635523732532624 No Hit TCTTAATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCC 209 0.3017092042961081 No Hit TCTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTAGTATGCCG 198 0.28582977249104974 No Hit CTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGACGT 177 0.2555144935904839 No Hit TTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGTC 165 0.23819147707587482 No Hit TATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGTCT 150 0.21653770643261347 RNA PCR Primer, Index 41 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGA 144 0.20787619817530892 No Hit TCTTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGAC 130 0.18766601224159835 No Hit CTTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCG 129 0.1862224275320476 No Hit TCTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGACG 118 0.17034299572698927 No Hit CTTATACACATCTCCGAGCCCACGAGACAATAAGAAATCTCGTATGCCGT 115 0.166012241598337 No Hit TCTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATTCCG 91 0.13136620856911885 No Hit CATATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGT 86 0.12414828502136505 No Hit TCTTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATTCC 78 0.112599607344959 No Hit CTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTAGTATGCCGT 77 0.11115602263540826 No Hit TCTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGT 75 0.10826885321630673 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.002887169419101513 0.0 5 0.0 0.0 0.0 0.005774338838203026 0.0 6 0.0 0.0 0.0 0.005774338838203026 0.0 7 0.0 0.0 0.0 0.005774338838203026 0.0 8 0.0 0.0 0.0 0.005774338838203026 0.0 9 0.0 0.0 0.0 0.007217923547753782 0.0 10 0.0 0.0 0.0 0.008661508257304539 0.0 11 0.0 0.0 0.0 0.008661508257304539 0.0 12 0.0 0.0 0.0 0.008661508257304539 0.0 13 0.0 0.0 0.0 0.010105092966855295 0.0 14 0.0 0.0 0.0 0.012992262385956807 0.0 15 0.0 0.0 0.0 0.01587943180505832 0.0 16 0.0 0.0 0.0 0.018766601224159834 0.0 17 0.0 0.0 0.0 0.02021018593371059 0.0 18 0.0 0.0 0.0 0.02742810948146437 0.0 19 0.0 0.0 0.0 0.060630557801131774 0.0 20 0.0 0.0 0.0 0.07073565076798706 0.0 21 0.0 0.0 0.0 0.07217923547753782 0.0 22 0.0 0.0 0.0 0.08661508257304538 0.0 23 0.0 0.0 0.0 0.09816376024945143 0.0 24 0.0 0.0 0.0 0.09816376024945143 0.0 25 0.0 0.0 0.0 0.10105092966855295 0.0 26 0.0 0.0 0.0 0.10105092966855295 0.0 27 0.0 0.0 0.0 0.10393809908765446 0.0 28 0.0 0.0 0.0 0.11404319205450976 0.0 29 0.0 0.0 0.0 0.11548677676406051 0.0 30 0.0 0.0 0.0 0.12847903915001732 0.0 31 0.0 0.0 0.0 0.14147130153597412 0.0 32 0.0 0.0 0.0 0.1559071486314817 0.0 33 0.0 0.0 0.0 0.19632752049890287 0.0 34 0.0 0.0 0.0 0.2454094006236286 0.0 35 0.0 0.0 0.0 0.29737845016745584 0.0 36 0.0 0.0 0.0 0.385437117450052 0.0 37 0.0 0.0 0.0 0.5182469107287215 0.0 38 0.0 0.0 0.0 0.6727104746506525 0.0 39 0.0 0.0 0.0 0.8170689456057282 0.0 40 0.0 0.0 0.0 0.9715325095276591 0.0 41 0.0 0.0 0.0 1.0855757015821688 0.0 42 0.0 0.0 0.0 1.2371520960849982 0.0 43 0.0 0.0 0.0 1.4912230049659314 0.0 44 0.0 0.0 0.0 1.700542787850791 0.0 45 0.0 0.0 0.0 1.9603880355699272 0.0 46 0.0 0.0 0.0 2.1827000808407435 0.0 47 0.0 0.0 0.0 2.381914770758748 0.0 48 0.0 0.0 0.0 2.5811294606767525 0.0 49 0.0 0.0 0.0 2.7817877353043077 0.0 50 0.0 0.0 0.0 2.959348654579051 0.0 51 0.0 0.0 0.0 3.229298995265042 0.0 52 0.0 0.0 0.0 3.4790391500173232 0.0 53 0.0 0.0 0.0 3.6667051622589213 0.0 54 0.0 0.0 0.0 3.9121145628825498 0.0 55 0.0 0.0 0.0 4.145975285829772 0.0 56 0.0 0.0 0.0 4.352407899295531 0.0 57 0.0 0.0 0.0 4.576163529275898 0.0 58 0.0 0.0 0.0 4.788370481579859 0.0 59 0.0 0.0 0.0 4.994803095045618 0.0 60 0.0 0.0 0.0 5.205566462640028 0.0 61 0.0 0.0 0.0 5.404781152558032 0.0 62 0.0 0.0 0.0 5.621318858990645 0.0 63 0.0 0.0 0.0 5.80032336297494 0.0 64 0.0 0.0 0.0 6.042845594179466 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAGCGTC 15 0.0022160618 70.0 2 AGCCGAC 15 0.0022160618 70.0 45 CGAAATA 20 7.876203E-5 70.0 48 CGTCTAC 25 2.8128816E-6 70.0 48 TCCTATG 15 0.0022160618 70.0 40 TGACGAA 15 0.0022160618 70.0 45 TGTGCCC 15 0.0022160618 70.0 18 AATGCCG 20 7.876203E-5 70.0 43 CGAATGA 15 0.0022160618 70.0 41 AAAGCCG 15 0.0022160618 70.0 43 GCCGACA 40 1.047556E-8 61.25 46 TCTCGAA 30 8.297577E-6 58.333332 38 CCGACAT 30 8.297577E-6 58.333332 47 GCCGAAT 25 2.3753263E-4 55.999996 47 TCCGTCA 25 2.3753263E-4 55.999996 48 CCGAATT 25 2.3753263E-4 55.999996 48 CTCCTAT 25 2.3753263E-4 55.999996 39 ACGAATG 25 2.3753263E-4 55.999996 40 AGCGTCA 20 0.0069246185 52.5 3 CGGTTGT 20 0.0069246185 52.5 25 >>END_MODULE