FastQCFastQC Report
Wed 25 May 2016
SRR1780137_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780137_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31535
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG641120.3297922942762No Hit
CTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGTG13324.223878230537498No Hit
ATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGTGGTC1740.5517678769621056No Hit
TCTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGT1230.3900428095766609No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGG800.25368638021246237No Hit
TCTTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGG590.18709370540669099No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACAAC150.00220481670.045
TCTAGTA150.00220481670.024
ACGTTTT150.00220481670.011
CACGCTA150.00220481670.023
CCACGCT150.00220481670.022
GTCCGAA150.00220481670.047
CACGACA150.00220481670.043
CCCACCA150.00220481670.061
GCTACTC150.00220481670.026
GAGTCCG150.00220481670.045
ACCTGCA150.00220481670.038
TAACCCT150.00220481670.016
CGAACTA150.00220481670.050
GCACGAC150.00220481670.042
CTAGTAA150.00220481670.025
TACCTGC150.00220481670.037
TCCGAAC150.00220481670.048
CTACCTG150.00220481670.036
CCGAACT150.00220481670.049
TGGTCGC2150.060.2325649
Produced by FastQC (version 0.11.5)