FastQCFastQC Report
Wed 25 May 2016
SRR1780137_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780137_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31535
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23827.553511970826065No Hit
ATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGTCTT8422.670049151736166Illumina PCR Primer Index 5 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGT7622.416362771523704No Hit
TCTTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCG4751.5062628825114952No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCC3601.1415887109560807No Hit
CTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGTC3451.094022514666244No Hit
CTTTACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGTC2830.8974155700015856No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCC1320.41858252735056284No Hit
TATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGTCT1280.4058982083399398Illumina PCR Primer Index 5 (95% over 21bp)
CTTAATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCG1260.39955604883462825No Hit
TTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGTC1020.3234501347708895No Hit
ATTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGT780.24734422070715076No Hit
CATATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGT570.18075154590137943No Hit
TCTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGT510.16172506738544476No Hit
CTTTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCG470.14904074837482162No Hit
CTTATACACATCTCCGAGCCCACGAGACCACAATTGATCTCGTATGCCGT410.13001426985888695No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCAATGATCTCGTATGCCGT380.12050103060091961No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGATC252.7803271E-669.98730533
CATAGAT207.8167315E-569.98730532
CCATAGA207.8167315E-569.98730531
AGATCTC252.7803271E-669.98730535
TCTTTAT501.2732926E-1162.988571
TTTACAC308.20204E-658.322752
TCTTAAT308.20204E-658.322751
AACTGAA200.00685141252.57392526
CTATACA800.052.4904751
TTTATAC608.0035534E-1152.4904753
CTTTATA651.7462298E-1048.4527472
ACCATAG305.79746E-446.658230
CACGTAT305.79746E-446.658239
ACGTATG305.79746E-446.658240
TAGATCT404.4981633E-543.74206534
CTTTACA459.009336E-538.8818361
TTACACA459.009336E-538.8818363
CATATAC459.009336E-538.8818361
ATTATAC601.1809556E-534.9936521
GGGGGTA400.002386131334.9936521
Produced by FastQC (version 0.11.5)