##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780137_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 31535 Sequences flagged as poor quality 0 Sequence length 76 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.409608371650545 27.0 27.0 32.0 13.0 32.0 2 26.770952909465674 32.0 27.0 32.0 13.0 32.0 3 27.320723006183606 32.0 27.0 32.0 13.0 32.0 4 29.468305057872204 32.0 32.0 32.0 22.0 32.0 5 28.469256381798 32.0 27.0 32.0 13.0 32.0 6 31.595528777548754 37.0 32.0 37.0 13.0 37.0 7 33.163976534009834 37.0 32.0 37.0 22.0 37.0 8 32.27810369430791 37.0 32.0 37.0 13.0 37.0 9 33.214904074837484 37.0 32.0 37.0 22.0 37.0 10-11 33.09890597748533 37.0 32.0 37.0 22.0 37.0 12-13 33.02841287458379 37.0 32.0 37.0 22.0 37.0 14-15 33.03513556365942 37.0 32.0 37.0 22.0 37.0 16-17 32.25912478198826 37.0 32.0 37.0 13.0 37.0 18-19 32.3673537339464 37.0 32.0 37.0 13.0 37.0 20-21 32.893245600126846 37.0 32.0 37.0 22.0 37.0 22-23 32.30004756619629 37.0 32.0 37.0 17.5 37.0 24-25 31.869985730141114 37.0 29.5 37.0 13.0 37.0 26-27 31.78636435706358 37.0 32.0 37.0 13.0 37.0 28-29 31.92161090851435 37.0 32.0 37.0 13.0 37.0 30-31 31.88917076264468 37.0 32.0 37.0 13.0 37.0 32-33 31.775709529094655 37.0 32.0 37.0 13.0 37.0 34-35 31.230014269858884 37.0 27.0 37.0 13.0 37.0 36-37 31.17190423339147 37.0 29.5 37.0 13.0 37.0 38-39 30.591644204851754 37.0 27.0 37.0 13.0 37.0 40-41 30.960329792294274 37.0 29.5 37.0 13.0 37.0 42-43 31.42736641826542 37.0 29.5 37.0 13.0 37.0 44-45 31.329681306484858 37.0 27.0 37.0 13.0 37.0 46-47 30.618598382749326 37.0 27.0 37.0 13.0 37.0 48-49 30.131536388140162 37.0 24.5 37.0 13.0 37.0 50-51 29.66594260345648 37.0 22.0 37.0 13.0 37.0 52-53 29.807991120976695 37.0 22.0 37.0 13.0 37.0 54-55 29.649643253527827 37.0 22.0 37.0 13.0 37.0 56-57 28.613540510543842 34.5 22.0 37.0 13.0 37.0 58-59 29.01996194704297 32.0 22.0 37.0 13.0 37.0 60-61 28.389979387981608 32.0 22.0 37.0 13.0 37.0 62-63 28.993007769145393 34.5 22.0 37.0 13.0 37.0 64-65 28.84076423022039 32.0 22.0 37.0 13.0 37.0 66-67 28.403789440304422 32.0 22.0 37.0 13.0 37.0 68-69 27.645489139051847 32.0 13.0 37.0 13.0 37.0 70-71 25.95110195021405 29.5 13.0 37.0 10.5 37.0 72-73 25.514983351831297 32.0 13.0 37.0 8.0 37.0 74-75 26.174044712224514 32.0 13.0 37.0 8.0 37.0 76 26.23954336451562 32.0 13.0 37.0 8.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 11101 1 0.07929874968816364 11101 2 1.9269644385737799 11101 3 0.4255480666839553 11101 4 2.0374351162536826 11101 5 1.3415241456827864 11101 6 -2.4677449835398697 11101 7 -0.497448178492796 11101 8 -1.1307503561695498 11101 9 -3.5051733026779246 11101 10-11 0.6750888655239606 11101 12-13 0.24915314140980627 11101 14-15 -0.5779799795115466 11101 16-17 1.9304034177700444 11101 18-19 0.3957984445521845 11101 20-21 -0.8471433783493865 11101 22-23 0.4931798388023054 11101 24-25 0.10188921407122109 11101 26-27 1.684862200874015 11101 28-29 -1.9272370030856791 11101 30-31 -0.30885956263748326 11101 32-33 1.558209267074556 11101 34-35 1.0414476698017872 11101 36-37 1.7088023633275498 11101 38-39 1.6066719602935429 11101 40-41 0.07357661959101236 11101 42-43 -1.3508169282246172 11101 44-45 1.0834545878724064 11101 46-47 1.5910747717847329 11101 48-49 1.408890630005672 11101 50-51 -0.9107672129250659 11101 52-53 1.5125349478766772 11101 54-55 1.502821722169454 11101 56-57 3.30615615457139 11101 58-59 -0.6811142071139855 11101 60-61 2.4844997467539756 11101 62-63 -2.6940182294656694 11101 64-65 -1.5491519255329713 11101 66-67 -2.558490819620591 11101 68-69 -0.8130549338950992 11101 70-71 3.0456371704568426 11101 72-73 1.1931233560935688 11101 74-75 1.0517239893879484 11101 76 5.358618395071726 11102 1 -0.47839355800414296 11102 2 2.936579823189163 11102 3 0.0024711436070319337 11102 4 0.4316658854844526 11102 5 -3.605591238932597 11102 6 2.791870401075517 11102 7 0.6804364368918172 11102 8 -6.284596510015703 11102 9 -2.7119040719086946 11102 10-11 0.6270119424470408 11102 12-13 0.9967492952559596 11102 14-15 0.79221232818076 11102 16-17 0.9039611100777378 11102 18-19 -2.0969900169862754 11102 20-21 -0.35435491681092657 11102 22-23 -0.6895124688900012 11102 24-25 -2.366860785928779 11102 26-27 -3.9353301068182915 11102 28-29 -2.6844485415472192 11102 30-31 -5.56126340879133 11102 32-33 -1.0451561175408273 11102 34-35 -1.965763868659753 11102 36-37 1.006879286404473 11102 38-39 1.4359988833704662 11102 40-41 1.3692496965140926 11102 42-43 1.3991830717753828 11102 44-45 2.60268535710318 11102 46-47 0.8506901564001161 11102 48-49 -3.5454362930712513 11102 50-51 1.2382712486133975 11102 52-53 -1.985061205969476 11102 54-55 2.1398409529386875 11102 56-57 -1.0712476915824567 11102 58-59 1.8164819467321678 11102 60-61 -1.9505964070921777 11102 62-63 0.7098279243804839 11102 64-65 -0.8977096178406647 11102 66-67 0.047278411148639066 11102 68-69 -1.5462280108181758 11102 70-71 0.03361793968761262 11102 72-73 -0.013607413137201263 11102 74-75 0.2728778355417951 11102 76 1.416310702764033 11103 1 -1.9040345836451706 11103 2 -0.9147022280928852 11103 3 -2.7411185999827126 11103 4 0.729101782920349 11103 5 -1.9543091876505478 11103 6 -2.7260783168732026 11103 7 -2.801614845159463 11103 8 -1.6640836895028848 11103 9 1.0573266973220754 11103 10-11 0.15008886552396206 11103 12-13 1.0762364747431405 11103 14-15 0.7178533538217877 11103 16-17 -0.821679915563287 11103 18-19 1.2978817778855216 11103 20-21 -2.0158933783493858 11103 22-23 1.0515131721356425 11103 24-25 -1.3335274525954475 11103 26-27 0.8056955342073522 11103 28-29 0.9540129969143223 11103 30-31 -0.38177622930414756 11103 32-33 0.2727926004078931 11103 34-35 -0.9273023301982128 11103 36-37 -0.41619763667245024 11103 38-39 1.94625529362688 11103 40-41 -0.7076733804089876 11103 42-43 -2.9674835948912843 11103 44-45 -1.3665454121275964 11103 46-47 -2.6005918948819335 11103 48-49 0.21097396333900775 11103 50-51 0.7433994537415991 11103 52-53 -1.8645483854566542 11103 54-55 -1.7909282778305453 11103 56-57 -2.72509384542861 11103 58-59 0.2626357928860159 11103 60-61 -1.3967502532460259 11103 62-63 -2.6106848961323372 11103 64-65 0.3279314078003601 11103 66-67 1.6498425137127448 11103 68-69 -3.2026382672284335 11103 70-71 0.297720503790174 11103 72-73 -0.7110433105730998 11103 74-75 -0.4066093439453873 11103 76 4.241951728405059 11104 1 3.1822399261587506 11104 2 2.7284350268090733 11104 3 0.39613630197807126 11104 4 0.13302335154779854 11104 5 -1.5739170307878005 11104 6 1.1131373694013043 11104 7 -4.6408305314339735 11104 8 -2.3072209444048433 11104 9 -3.354438008560276 11104 10-11 -0.6557934874172133 11104 12-13 -2.5100380350607807 11104 14-15 0.6904023734296274 11104 16-17 0.5351828295347545 11104 18-19 -2.2825103789772285 11104 20-21 -1.4335404371729155 11104 22-23 0.23582689762583442 11104 24-25 -1.4550960800464239 11104 26-27 -0.4511672108906879 11104 28-29 1.2951894675025528 11104 30-31 -1.3033448567551282 11104 32-33 -0.13112896821956 11104 34-35 1.8962270815664901 11104 36-37 -2.5926682249077437 11104 38-39 0.3364513720582494 11104 40-41 -0.32532043923251663 11104 42-43 -0.7772875164599142 11104 44-45 -1.6077218827158255 11104 46-47 1.6131335953141388 11104 48-49 0.2894053358880271 11104 50-51 0.2767327870749341 11104 52-53 -0.6841562285939098 11104 54-55 1.1149540751106315 11104 56-57 0.392553213394919 11104 58-59 -1.543246560055163 11104 60-61 0.8208968055775046 11104 62-63 1.7324523587696277 11104 64-65 -0.2642254549447358 11104 66-67 -3.1246672902088264 11104 68-69 1.1777538896343138 11104 70-71 4.352622464574491 11104 72-73 3.8144468855053333 11104 74-75 -0.3379818929649936 11104 76 2.7556772186011393 11105 1 2.89596541635483 11105 2 -2.44803556142622 11105 3 -4.674451933316046 11105 4 -1.2042315504129846 11105 5 1.5123574790161207 11105 6 -1.326078316873204 11105 7 -1.468281511826131 11105 8 -1.6640836895028848 11105 9 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72-73 -3.177709977239765 11105 74-75 0.5600573227212813 11105 76 -0.8913816049282737 11106 1 -2.539122302943415 11106 2 -1.763825035110429 11106 3 -0.39024140700025356 11106 4 -0.8638806732200024 11106 5 -0.11571269642247728 11106 6 -2.750639720381976 11106 7 -2.619158704808587 11106 8 2.027144380672553 11106 9 -0.10056803952002724 11106 10-11 -1.344647976581303 11106 12-13 -0.9992021217480911 11106 14-15 -0.2523220847747041 11106 16-17 -1.2093992138089042 11106 18-19 0.15402212876271193 11106 20-21 1.973580305861141 11106 22-23 -0.7765570033029583 11106 24-25 -2.024755522770885 11106 26-27 0.24078325350559027 11106 28-29 1.1171708916511633 11106 30-31 0.6006799110467291 11106 32-33 -3.0815933645043927 11106 34-35 0.9411187224333624 11106 36-37 -0.33725026825139537 11106 38-39 1.3550272234514367 11106 40-41 -1.3918839067247752 11106 42-43 -2.179764296645672 11106 44-45 1.2808230089250365 11106 46-47 0.49239056125841785 11106 48-49 -1.569727791046958 11106 50-51 -1.4864251076619084 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10-11 -0.07491113447603936 11108 12-13 0.1929031414098077 11108 14-15 0.4095200204884506 11108 16-17 -0.9133465822299556 11108 18-19 1.9145484445521816 11108 20-21 0.5091066216506164 11108 22-23 0.3681798388023054 11108 24-25 0.8081392140712182 11108 26-27 -0.37763779912598494 11108 28-29 0.9290129969143237 11108 30-31 -0.7901095626374826 11108 32-33 0.714459267074556 11108 34-35 -2.2773023301982143 11108 36-37 0.0338023633275526 11108 38-39 1.2629219602935429 11108 40-41 -2.782673380408987 11108 42-43 -0.9008169282246179 11108 44-45 -1.9665454121275943 11108 46-47 1.7160747717847329 11108 48-49 0.32764063000567134 11108 50-51 0.3017327870749327 11108 52-53 2.3937849478766715 11108 54-55 -0.39092827783054673 11108 56-57 0.39990615457139 11108 58-59 -0.9123642071139884 11108 60-61 0.928249746753977 11108 62-63 0.030981770534332043 11108 64-65 -0.8554019255329699 11108 66-67 0.5415091803794105 11108 68-69 0.7306950661049036 11108 70-71 0.9393871704568397 11108 72-73 4.005623356093569 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44-45 1.6721642652917659 21610 46-47 0.6434941266234446 21610 48-49 2.9421567590379283 21610 50-51 1.131571496752354 21610 52-53 2.6849139801347413 21610 54-55 2.667136238298486 21610 56-57 0.6942609932810697 21610 58-59 -0.46639646517850153 21610 60-61 0.5580884564313955 21610 62-63 2.749530157631103 21610 64-65 1.2784690422089646 21610 66-67 1.526186599734249 21610 68-69 1.1113402273952246 21610 70-71 3.6192258801342625 21610 72-73 3.836268517383889 21610 74-75 1.9267239893879484 21610 76 3.0118442015233384 21611 1 0.12929874968816435 21611 2 0.6186311052404463 21611 3 -0.807785266649379 21611 4 0.39576844958701685 21611 5 1.5123574790161207 21611 6 -1.8927449835398704 21611 7 1.9317184881738712 21611 8 2.1025829771637845 21611 9 -0.14267330267792744 21611 10-11 0.5000888655239635 21611 12-13 0.6595698080764691 21611 14-15 1.951186687155122 21611 16-17 1.7616534177700416 21611 18-19 1.2978817778855216 21611 20-21 2.0507732883172807 21611 22-23 0.6348465054689711 21611 24-25 -0.33352745259544747 21611 26-27 -0.0943044657926535 21611 28-29 0.8540129969143209 21611 30-31 1.3682237706958524 21611 32-33 -0.3438740662587776 21611 34-35 0.6560310031351229 21611 36-37 2.183802363327551 21611 38-39 2.0295886269602086 21611 40-41 1.4756599529243495 21611 42-43 0.3158497384420471 21611 44-45 1.6667879212057422 21611 46-47 0.3827414384513972 21611 48-49 2.377640630005672 21611 50-51 -0.17326721292506875 21611 52-53 2.1187849478766765 21611 54-55 0.5424050555027868 21611 56-57 1.2249061545713893 21611 58-59 2.212635792886015 21611 60-61 0.553249746753977 21611 62-63 0.7559817705343299 21611 64-65 -0.3387352588663042 21611 66-67 -0.36682415295392445 21611 68-69 1.1640283994382337 21611 70-71 0.5643871704568397 21611 72-73 -0.21104331057309977 21611 74-75 0.8100573227212813 21611 76 1.908618395071727 21612 1 -0.3040345836451692 21612 2 -1.9813688947595551 21612 3 2.0255480666839567 21612 4 -0.8375648837463174 21612 5 0.8790241456827879 21612 6 0.207255016460131 21612 7 -2.7349481784927967 21612 8 1.9359163104971202 21612 9 -3.042673302677926 21612 10-11 -0.8332444678093722 21612 12-13 -1.0237635252568609 21612 14-15 -2.1488133128448794 21612 16-17 -0.5883465822299563 21612 18-19 -1.0854515554478148 21612 20-21 -1.7325600450160508 21612 22-23 0.8848465054689711 21612 24-25 -1.6835274525954453 21612 26-27 0.7556955342073479 21612 28-29 -0.2959870030856777 21612 30-31 0.2515571040291853 21612 32-33 -1.0938740662587776 21612 34-35 0.48936433646845146 21612 36-37 -1.366197636672453 21612 38-39 0.8129219602935436 21612 40-41 -2.7076733804089876 21612 42-43 0.48251640510871496 21612 44-45 -0.48321207879426353 21612 46-47 0.9327414384513979 21612 48-49 -1.7723593699943265 21612 50-51 -0.023267212925063063 21612 52-53 -2.564548385456657 21612 54-55 -0.39092827783054673 21612 56-57 -2.091760512095277 21612 58-59 0.39596912621934877 21612 60-61 -2.013416919912693 21612 62-63 0.05598177053433062 21612 64-65 0.4112647411336958 21612 66-67 1.9665091803794077 21612 68-69 -0.43597160056176776 21612 70-71 -0.3689461628764903 21612 72-73 1.8056233560935695 21612 74-75 0.24339065605461485 21612 76 0.875285061738392 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 2.0 17 8.0 18 14.0 19 69.0 20 155.0 21 276.0 22 518.0 23 813.0 24 1024.0 25 1366.0 26 1816.0 27 2148.0 28 2572.0 29 2793.0 30 3306.0 31 3289.0 32 3376.0 33 2965.0 34 2547.0 35 1823.0 36 655.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.400665926748054 29.142222926906612 14.840653242429047 16.616457903916285 2 29.752013699498953 23.43185133506691 25.791209488171496 21.024925477262638 3 31.797425001585587 27.86833259339126 25.74364178347181 14.590600621551342 4 22.68273347074679 20.155382907880135 39.549706675122884 17.612176946250198 5 22.6605359124782 43.374028856825745 27.29348343110829 6.6719517995877595 6 29.99207230061836 36.952592357697796 19.16600602505153 13.889329316632312 7 28.71412716029808 33.99714602822261 19.695576343745046 17.593150467734265 8 26.107499603615032 37.59315046773427 16.537180910099888 19.762169018550814 9 26.89709846202632 22.56223244014587 16.296178848898048 34.24449024892976 10-11 26.922467100047566 26.687807198351038 25.833201205010305 20.556524496591088 12-13 28.655462184873947 20.87838909148565 26.26763913112415 24.198509592516253 14-15 28.417631203424765 21.956556207388616 20.155382907880135 29.470429681306488 16-17 32.06278737910259 26.748057713651498 18.111622007293484 23.077532899952434 18-19 31.16378626922467 26.097986364357062 19.232598699857302 23.505628666560966 20-21 27.03504043126685 23.75614396702077 18.807674013001428 30.401141588710956 22-23 28.52703345489139 28.907563025210088 18.539717773902016 24.025685745996512 24-25 31.74250832408435 29.459330902172187 18.93293166323133 19.86522911051213 26-27 31.032459603888174 30.85010227867371 19.591519591519592 18.525918525918524 28-29 25.7547409145684 26.653770533392528 19.978435973869473 27.613052578169594 30-31 23.995560488346282 28.314571111463454 21.81861423814809 25.871254162042174 32-33 25.120504851905878 28.805416375975142 23.16705777890531 22.907020993213674 34-35 26.149138245413756 27.27806053687231 31.019993974852937 15.552807242860995 36-37 29.470429681306488 25.869668622165847 28.05612811162201 16.60377358490566 38-39 27.16188362137308 25.232281591882032 25.977485333756146 21.628349452988743 40-41 25.195814174726493 23.186935151419057 28.652291105121293 22.964959568733153 42-43 31.285872839701916 22.51625178373236 28.449342000951322 17.748533375614397 44-45 26.526082130965595 26.570477247502776 28.317742191216112 18.585698430315524 46-47 30.740447122245122 22.3592833359759 24.991279530680195 21.908990011098776 48-49 29.40066592674806 20.77374345964801 24.247661328682415 25.577929284921513 50-51 26.31203424766133 21.15110195021405 29.19771682257809 23.339146979546534 52-53 23.919454574282543 20.7388615823688 34.682099254796256 20.659584588552402 54-55 23.46440462977644 21.3017282384652 32.54637704138259 22.68749009037577 56-57 28.1750436023466 21.21135246551451 27.605834786744886 23.007769145394004 58-59 24.659901696527665 25.34009830347233 30.742032662121453 19.257967337878547 60-61 26.63231330267956 32.30537498018075 24.918344696369115 16.14396702077057 62-63 22.66687807198351 41.65847471063897 19.51958141747265 16.155065799904868 64-65 20.97669256381798 43.35341683843348 19.00110987791343 16.668780719835105 66-67 21.21961661037911 43.96771789627233 18.299005850734908 16.513659642613646 68-69 24.56001268431901 40.31076581576027 18.546059933407324 16.5831615665134 70-71 29.78753765657206 33.90994133502458 18.601553829078803 17.700967179324557 72-73 39.18344696369114 24.261931187569367 18.179800221975583 18.37482162676391 74-75 41.38259077215792 23.106072617726337 17.36958934517203 18.141747264943714 76 41.58871095608055 23.24718566671952 17.850007927699384 17.314095449500556 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 1.0 9 1.0 10 1.5 11 2.5 12 3.0 13 4.0 14 7.5 15 15.0 16 23.0 17 26.0 18 34.5 19 45.5 20 52.0 21 56.0 22 68.0 23 107.0 24 158.5 25 183.0 26 197.5 27 232.5 28 309.0 29 365.0 30 397.5 31 493.0 32 598.0 33 640.0 34 728.5 35 800.5 36 884.0 37 984.0 38 1085.5 39 1220.5 40 1322.5 41 1421.0 42 1451.0 43 1567.0 44 1772.0 45 2190.5 46 2520.0 47 2573.0 48 2485.5 49 2099.0 50 1853.0 51 1813.0 52 1239.5 53 662.5 54 619.0 55 528.0 56 386.5 57 282.0 58 228.0 59 197.5 60 154.0 61 129.5 62 118.0 63 97.0 64 73.5 65 68.5 66 63.5 67 61.0 68 59.5 69 59.0 70 51.5 71 43.0 72 35.5 73 26.0 74 25.0 75 26.0 76 27.0 77 22.0 78 17.5 79 19.0 80 18.0 81 14.5 82 11.0 83 10.0 84 10.0 85 6.0 86 3.0 87 4.0 88 4.0 89 4.5 90 4.0 91 6.0 92 9.0 93 14.0 94 17.5 95 21.0 96 26.0 97 35.5 98 78.5 99 1214.0 100 2316.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0031710797526557795 3 0.0031710797526557795 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.011098779134295227 28-29 0.0031710797526557795 30-31 0.0 32-33 0.0031710797526557795 34-35 0.0015855398763278898 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0015855398763278898 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 31535.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.86982717615348 #Duplication Level Percentage of deduplicated Percentage of total 1 85.84686774941996 53.97177739020136 2 8.473721375970946 10.654827968923417 3 2.769091092504792 5.222768352624069 4 1.2357510339957631 3.107658157602664 5 0.6456168667406436 2.029491041699699 6 0.3682033693130233 1.3889329316632313 7 0.17653586199939472 0.776914539400666 8 0.11096539897104812 0.5581100364674172 9 0.08574599011399173 0.48517520215633425 >10 0.21688691617068495 2.387823053749802 >50 0.015131645314233834 0.5898208339939749 >100 0.04035105417129022 6.186776597431425 >500 0.010087763542822556 5.0864119232598695 >1k 0.005043881771411278 7.553511970826065 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 2382 7.553511970826065 No Hit ATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGTCTT 842 2.670049151736166 Illumina PCR Primer Index 5 (95% over 22bp) CTTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGT 762 2.416362771523704 No Hit TCTTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCG 475 1.5062628825114952 No Hit TCTTTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCC 360 1.1415887109560807 No Hit CTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGTC 345 1.094022514666244 No Hit CTTTACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGTC 283 0.8974155700015856 No Hit TCTTAATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCC 132 0.41858252735056284 No Hit TATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGTCT 128 0.4058982083399398 Illumina PCR Primer Index 5 (95% over 21bp) CTTAATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCG 126 0.39955604883462825 No Hit TTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGTC 102 0.3234501347708895 No Hit ATTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGT 78 0.24734422070715076 No Hit CATATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGT 57 0.18075154590137943 No Hit TCTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGT 51 0.16172506738544476 No Hit CTTTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCG 47 0.14904074837482162 No Hit CTTATACACATCTCCGAGCCCACGAGACCACAATTGATCTCGTATGCCGT 41 0.13001426985888695 No Hit CTTATACACATCTCCGAGCCCACGAGACCACCAATGATCTCGTATGCCGT 38 0.12050103060091961 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.006342159505311558 0.0 10 0.0 0.0 0.0 0.006342159505311558 0.0 11 0.0 0.0 0.0 0.006342159505311558 0.0 12 0.0 0.0 0.0 0.006342159505311558 0.0 13 0.0 0.0 0.0 0.006342159505311558 0.0 14 0.0 0.0 0.0 0.009513239257967338 0.0 15 0.0 0.0 0.0 0.009513239257967338 0.0 16 0.0 0.0 0.0 0.009513239257967338 0.0 17 0.0 0.0 0.0 0.009513239257967338 0.0 18 0.0 0.0 0.0 0.009513239257967338 0.0 19 0.0 0.0 0.0 0.050737276042492466 0.0 20 0.0 0.0 0.0 0.06342159505311559 0.0 21 0.0 0.0 0.0 0.07927699381639448 0.0 22 0.0 0.0 0.0 0.0919613128270176 0.0 23 0.0 0.0 0.0 0.09830347233232915 0.0 24 0.0 0.0 0.0 0.09830347233232915 0.0 25 0.0 0.0 0.0 0.09830347233232915 0.0 26 0.0 0.0 0.0 0.09830347233232915 0.0 27 0.0 0.0 0.0 0.11098779134295228 0.0 28 0.0 0.0 0.0 0.12367211035357539 0.0 29 0.0 0.0 0.0 0.12684319010623119 0.0 30 0.0 0.0 0.0 0.1522118281274774 0.0 31 0.0 0.0 0.0 0.15538290788013318 0.0 32 0.0 0.0 0.0 0.18075154590137943 0.0 33 0.0 0.0 0.0 0.22831774219121612 0.0 34 0.0 0.0 0.0 0.25368638021246237 0.0 35 0.0 0.0 0.0 0.32979229427620105 0.0 36 0.0 0.0 0.0 0.42175360710321863 0.0 37 0.0 0.0 0.0 0.6627556683050578 0.0 38 0.0 0.0 0.0 0.8435072142064373 0.0 39 0.0 0.0 0.0 1.090851434913588 0.0 40 0.0 0.0 0.0 1.4777231647375932 0.0 41 0.0 0.0 0.0 1.9058189313461233 0.0 42 0.0 0.0 0.0 2.3085460599334073 0.0 43 0.0 0.0 0.0 2.717615348026003 0.0 44 0.0 0.0 0.0 3.1393689551292216 0.0 45 0.0 0.0 0.0 3.659426034564769 0.0 46 0.0 0.0 0.0 4.112890439194546 0.0 47 0.0 0.0 0.0 4.743935309973046 0.0 48 0.0 0.0 0.0 5.2386237513873475 0.0 49 0.0 0.0 0.0 5.822102425876011 0.0 50 0.0 0.0 0.0 6.275566830505787 0.0 51 0.0 0.0 0.0 6.833676866973204 0.0 52 0.0 0.0 0.0 7.420326621214524 0.0 53 0.0 0.0 0.0 7.991120976692564 0.0 54 0.0 0.0 0.0 8.54288885365467 0.0 55 0.0 0.0 0.0 8.958300301252576 0.0 56 0.0 0.0 0.0 9.405422546377041 0.0 57 0.0 0.0 0.0 9.820833993974949 0.0 58 0.0 0.0 0.0 10.191850325035675 0.0 59 0.0 0.0 0.0 10.57872205485968 0.0 60 0.0 0.0 0.0 10.949738385920407 0.0 61 0.0 0.0 0.0 11.365149833518313 0.0 62 0.0 0.0 0.0 11.745679403837007 0.0 63 0.0 0.0 0.0 12.129380053908356 0.0 64 0.0 0.0 0.0 12.541620421753606 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATAGATC 25 2.7803271E-6 69.987305 33 CATAGAT 20 7.8167315E-5 69.987305 32 CCATAGA 20 7.8167315E-5 69.987305 31 AGATCTC 25 2.7803271E-6 69.987305 35 TCTTTAT 50 1.2732926E-11 62.98857 1 TTTACAC 30 8.20204E-6 58.32275 2 TCTTAAT 30 8.20204E-6 58.32275 1 AACTGAA 20 0.006851412 52.573925 26 CTATACA 80 0.0 52.490475 1 TTTATAC 60 8.0035534E-11 52.490475 3 CTTTATA 65 1.7462298E-10 48.452747 2 ACCATAG 30 5.79746E-4 46.6582 30 CACGTAT 30 5.79746E-4 46.6582 39 ACGTATG 30 5.79746E-4 46.6582 40 TAGATCT 40 4.4981633E-5 43.742065 34 CTTTACA 45 9.009336E-5 38.881836 1 TTACACA 45 9.009336E-5 38.881836 3 CATATAC 45 9.009336E-5 38.881836 1 ATTATAC 60 1.1809556E-5 34.993652 1 GGGGGTA 40 0.0023861313 34.993652 1 >>END_MODULE