FastQCFastQC Report
Wed 25 May 2016
SRR1780136_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780136_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76691
Sequences flagged as poor quality0
Sequence length76
%GC87

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5354969.82436009440482No Hit
CTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGTG1003613.086281310714426No Hit
TCTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGT8801.1474618925297622No Hit
ATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGTGGTC4140.5398286630765018No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGG3870.5046224459193387No Hit
TCTTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGG3060.3990037944478492No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1800.23470811438108774No Hit
CTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGTGG1140.14864847244135557No Hit
CTTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGT930.12126585909689534No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG880.11474618925297624No Hit
CTTAATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGT820.1069225854402733No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATGCA150.002211724270.041782
TGCACAT150.002211724270.041785
CTTTACA150.002211724270.041781
TTGTATA150.002211724270.041784
AAACAAC150.002211724270.041786
CTTATGC207.858289E-570.041781
ATGCACA150.002211724270.041784
TCTTTAT150.002211724270.041781
GCACATC207.858289E-570.041786
TTTACAC150.002211724270.041782
TGTATAT150.002211724270.041785
TGTGCGC150.002211724270.041783
GTTGTAT150.002211724270.041783
CGCTGGC252.816696E-669.99608616
CGCTGAC207.8837584E-569.99608616
GAGGTCG207.8837584E-569.99608648
TGGTCGA207.8837584E-569.99608649
AACAACT150.002217460769.9960867
ACGCTGA207.8837584E-569.99608615
CGGAGGT207.8837584E-569.99608646
Produced by FastQC (version 0.11.5)