FastQCFastQC Report
Wed 25 May 2016
SRR1780136_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780136_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76691
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGT2999239.10758759176435No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1461119.051779217900407No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCG55947.294206621376693No Hit
ATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGTCTT15021.9585088211132986TruSeq Adapter, Index 8 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCC11941.556897158727882No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCC11121.4499745732876088No Hit
CATATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGT8241.0744415902778683No Hit
ATTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGT8041.048362910902192No Hit
CTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGTC6740.8788514949602952No Hit
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGT5980.7797525133327248No Hit
CTTTACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGTC4480.5841624180151517No Hit
CTTATACACATCTCCGAGCCCACGAGACAGAATTAAATCTCGTATGCCGT3880.5059263798881225No Hit
CTTTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCG3510.45768082304312113No Hit
CTTAATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCG3490.45507295510555346No Hit
CTTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGACGT3100.4042195303229844No Hit
CTTATACACATCTCCGAGCCCACGAGACAGATTAAAATCTCGTATGCCGT2880.3755329830097404No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCC2790.363797577290686No Hit
TTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGTC2650.3455425017277125No Hit
TATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGTCT2470.3220716902896037No Hit
CTTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGA2400.31294415250811697No Hit
CCTTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCG1990.25948285978798036No Hit
CTAATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGT1700.22166877469324953No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCAG1570.20471763309905985No Hit
CTTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCAGT1500.1955900953175731No Hit
CTTATACACATCTCCGAGCCCACGAGACAGATTTAATCTCGTATGCCGTC1460.19037435944243783No Hit
CTTATACACATCTCCGAGCCCACGAGACAGATTTAAATATCGTATGCCGT1260.1642956800667614No Hit
CTTATACACATCTCCGAGCCCACGAGACAGAAATAAATCTCGTATGCCGT1070.1395209346598688No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGACG960.1251776610032468No Hit
CTTATACACATCTCCGAGCCAACGAGACAGATTTAAATCTCGTATGCCGT960.1251776610032468No Hit
TCTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGT890.11605012322176006No Hit
CTTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGAATGCCGT880.11474618925297624No Hit
AATATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGT850.11083438734662476No Hit
CTTATACACATCACCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGT840.10953045337784095No Hit
CTTATACACATCTCCGAGCCCACGAGACAGATTTAAAACTCGTATGCCGT820.1069225854402733No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGATTTAACTCTCGTATGCCG820.1069225854402733No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGTG207.865185E-570.0293770
TTGCAAA150.00221327870.0293758
TAAATAA150.00221327870.0293734
TGCAAAA150.00221327870.0293759
AGGGTGG150.00221327870.0293770
CACGAAT150.00221327870.0293739
AGGTGGG150.00221327870.0293770
GCAAAAA150.00221327870.0293760
AGCGGGG150.00221327870.0293770
GCCGTAT150.00221327870.0293746
ACGAATG150.00221327870.0293740
AATGCCG550.070.0293743
AAAAACT150.00221327870.0293734
ACTGCTA301.0132135E-769.98369653
CTTTTAT150.002219018569.9836961
ACTGCAT207.890677E-569.98369653
TCCTTAT207.890677E-569.9836961
TTCACAT207.890677E-569.9836965
AATCTCC500.069.9836969
TAAACAA252.8196591E-669.9836965
Produced by FastQC (version 0.11.5)