FastQCFastQC Report
Wed 25 May 2016
SRR1780135_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780135_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89127
Sequences flagged as poor quality0
Sequence length76
%GC80

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5216258.52547488415407No Hit
CTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGTG34253.8428310164147788No Hit
TCTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGT2910.32650038708808776No Hit
ATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGTGGTC2560.28723058108092947No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGG1990.22327689701212874No Hit
TCTTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGG1360.15259124619924377No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGTA207.888608E-570.053
GATAAAC150.002218156670.054
CCGGCGA150.002218156670.021
GTATCAA252.8195282E-670.057
GTTCAGT150.002218156670.047
TGGTGCG150.002218156670.02
GGTAGCC207.888608E-570.050
TGGTAGC207.888608E-570.049
TGGCGCT308.317085E-658.33333213
TAGCCGT252.3790445E-455.99999652
GTAGCCG252.3790445E-455.99999651
GTAGATA252.3790445E-455.99999638
GGTGGTA252.3790445E-455.99999647
TCTTTAT252.3790445E-455.9999961
GTGGTAG252.3790445E-455.99999648
AGGTGGT252.3790445E-455.99999646
AAAAAGG4100.054.63414870
ACAAATC200.00693111352.58
CCCTCCA200.00693111352.557
GCGGTGG200.00693111352.545
Produced by FastQC (version 0.11.5)