FastQCFastQC Report
Wed 25 May 2016
SRR1780135_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780135_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89127
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGT2846231.93420624502115No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG58736.589473448001167No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCG50895.709829793440821No Hit
ATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGTCTT13961.5663042624569432TruSeq Adapter, Index 18 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCC12121.3598572823050254No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCC11201.2566337922290665No Hit
CTTATACACATCTCCGAGCCCACGAGACACGGGCGCATCTCGTATGCCGT8931.001941050411211No Hit
CATATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGT7700.8639357321574831No Hit
CTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGTC6740.7562242642521346No Hit
ATTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGT6120.686660607896597No Hit
CTTTACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGTC3750.4207479215052678No Hit
CTTATACACATCTCCGAGCCCACGAGACCCGGGAGCATCTCGTATGCCGT3390.38035612104076205No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCC2840.31864642588665615No Hit
CTTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCG2790.3130364535999192No Hit
CTTAATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCG2500.2804986143368452No Hit
CTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGACGT2460.27601063650745566No Hit
TCTTATACACATCTCCGCGCCCACGAGACCCGGGCGCATCTCGTATGCCG2340.2625467030192871No Hit
TATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGTCT2180.244594791701729No Hit
TTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGTC2130.23898481941499208No Hit
CTTATACACATCTCCGAGCCCACGAGCCCCGGGCGCATCTCGTATGCCGT1950.2187889191827392No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGGGCGCATCTCGTATGCCGT1910.21430094135334973No Hit
TCTTATACACATCTCCGAGCCCACGAGCCCCGGGCGCATCTCGTATGCCG1800.20195900232252853No Hit
CTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGA1720.19298304666374946No Hit
CTTATACACATCTCCGCGCCCACGAGACCCGGGCGCATCTCGTATGCCGT1680.18849506883435996No Hit
CCTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCG1590.17839711871823352No Hit
CTAATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGT1240.1391273127110752No Hit
CTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTAAGCCGT1240.1391273127110752No Hit
CTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCAGT1160.13015135705229616No Hit
TCTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGT1120.12566337922290666No Hit
TCTTATACACATCTCCGAGCCCACGAGACACGGGCGCATCTCGTATGCCG980.1099554568200433No Hit
CTTATACACATCTCCGAGCCCACGAGACCCGGGCGCAACTCGTATGCCGT940.10546747899065377No Hit
ATACACATCTCCGCGCCCACGAGACCCGGGCGCATCTCGTATGCCGTCTT930.1043454845333064TruSeq Adapter, Index 18 (95% over 21bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGGG252.8195282E-670.027
CCCTCTT150.002218156670.047
TTTACAC353.6616257E-970.02
TCTTAAT353.6616257E-970.01
TTCAAAA150.002218156670.058
TGACGAC207.888608E-570.045
AGAACCG301.01346814E-770.025
TAGCAGT150.002218156670.014
GCCGACA207.888608E-570.046
ATGACGA207.888608E-570.044
CCGACAT207.888608E-570.047
GACGACT207.888608E-570.046
GGTTACT150.002218156670.034
CCTTGAA207.888608E-570.056
ATTATAC1400.065.01
TCTTTAT1300.064.615391
ATATACA3500.062.02
AGGGGGG37450.061.9626270
ATATCGT401.0522854E-861.2537
TTACACA401.0522854E-861.253
Produced by FastQC (version 0.11.5)