FastQCFastQC Report
Wed 25 May 2016
SRR1780134_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780134_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences227856
Sequences flagged as poor quality0
Sequence length76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7245731.79946984060108No Hit
CTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGTG62422.73944947686258No Hit
TCTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGT5270.23128642651499193No Hit
ATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGTGGTC4500.1974931535706762No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGG3280.14395056526929287No Hit
TCTTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGG2370.10401306088055613No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGA150.002220868670.01382417
CTACATG150.002220868670.01382416
TCGCCGT9750.055.28083852
GCCGTAT9750.055.28083854
GTCGCCG9950.054.87316551
TGGTCGC9900.054.7967849
CGCCGTA9850.054.71961253
CCGTATC9850.054.3642955
ATCTCGG9900.053.736242
CGTATCA10000.053.54882456
CGGTGGT10000.053.54882446
GTATCAT9750.053.48600457
TAGATCT10100.053.3651739
GTAGATC10250.053.26712438
CATTAAA9800.053.2131261
TCGGTGG10000.053.1988345
TCTCGGT9950.053.1144143
AGATCTC10050.052.93416640
GATCTCG10050.052.93416641
CTCGGTG10000.052.8488444