FastQCFastQC Report
Wed 25 May 2016
SRR1780134_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780134_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences227856
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCGT4131218.130749245137277No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG98634.3286110525946215No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCG49292.163208342110807No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTAGTATGCCGT18100.7943613510287199No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCC16990.745646373147953No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGACGT13600.5968681974580438No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCC8560.3756758654588863No Hit
ATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCGTCTT8140.3572431711256232RNA PCR Primer, Index 26 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCGA6500.28526788849097673No Hit
CTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCGTC6280.2756126676497437No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTAAGCCGT6050.26551857313390914No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTAGTATGCCG5600.24576925777684153No Hit
CATATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCGT5560.2440137630784355No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCCT5010.21987571097535288No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATTCCGT4540.1992486482690822No Hit
ATTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCGT4180.18344919598342813No Hit
CTTTACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCGTC4020.1764272171898041No Hit
ATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCGTCAT3900.17116073309458604No Hit
CTTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCG3720.16326100695175902No Hit
CTTATACACATCTCCGAGCCCACGAGACGATCGAGTATCTCGTATGCCGT3250.14263394424548836No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTCGAGAATCTCGTATGCCGT2950.1294677340074433No Hit
CTTAATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCG2700.11849589214240573No Hit
CCTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCG2670.11717927111860123No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCGT150.002218464670.03293630
CGTATCT207.913771E-570.002234
CGACATA252.833156E-670.002248
CGTCAAC207.913771E-570.002248
ATTACGG207.922381E-569.986833
AACTCGT353.7016434E-969.9868337
TAACTCG301.02192644E-769.9868336
AGGGGGG47750.062.2323270
CGAATGC800.061.2384841
ATGCCGG308.367959E-658.3223644
TCTTTAT1900.057.094521
AATCTCG1050.056.6560136
AAGCATG2800.056.25176254
CACTAAC252.3814125E-456.0263527
CTTAAGC2750.056.00175551
TTAAGCA1500.056.00175552
GCGTATC252.3865864E-456.00175533
CAGCTTG7500.056.0017554
CGGCATT252.3891767E-455.9894640
AAAACGG252.3891767E-455.9894667
Produced by FastQC (version 0.11.5)