FastQCFastQC Report
Wed 25 May 2016
SRR1780133_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780133_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences213389
Sequences flagged as poor quality0
Sequence length76
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7470735.009770887908935No Hit
CTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGTG47872.243320883456973No Hit
TCTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGT4140.1940118750263603No Hit
ATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGTGGTC3570.16730009513142666No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGG2810.1316843886048484No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTCTT150.00222241570.042
CGGTGTC150.00222241570.055
CGCGTCT150.00222241570.07
CCCTGTG207.91385E-570.032
GGAATGA252.3866104E-456.00000432
GCACCCT252.3866104E-456.00000429
TAGGTCA200.00694431952.531
CCGGAAT200.00694431952.553
TACATCT200.00694431952.539
ATCCGGA200.00694431952.551
TGACCAC200.00694431952.511
ACTACTG200.00694431952.553
GTTCCTA200.00694431952.529
ACATACG200.00694431952.521
CTCGGTG7850.050.38216844
GTAGATC7900.050.06328638
GGCCATT352.0817091E-550.00000444
CTGTGTA7850.049.9363134
TCGGTGG8000.049.87500445
ATCTCGG7900.049.6202542
Produced by FastQC (version 0.11.5)