FastQCFastQC Report
Wed 25 May 2016
SRR1780133_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780133_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences213389
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGT5088023.843778264109208No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG76643.5915628265749406No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCG61922.901742826481215No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCC25581.19874970124983No Hit
ATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGTCTT13750.6443631115005929RNA PCR Primer, Index 39 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCC11310.5300179484415786No Hit
CATATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGT8680.40676885875091967No Hit
CTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGTC7530.3528766712435974No Hit
ATTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGT5450.2554021060129622No Hit
CTTTACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGTC4890.22915895383548357No Hit
CTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGACGT4710.22072365492129398No Hit
CTTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCG3700.17339225545834133No Hit
CTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTAAGCCGT3580.1677687228488816No Hit
CTTAATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCG2990.140119687519038No Hit
CCTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCG2820.13215301632230342No Hit
CTTATACACATCTCCGAGCCCACGAGACGCAAGTACATCTCGTATGCCGT2700.12652948371284367No Hit
TTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGTC2540.11903144023356406No Hit
TATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGTCT2430.11387653534155931RNA PCR Primer, Index 39 (95% over 21bp)
TCTTAATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCC2420.11340790762410433No Hit
CTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGA2400.11247065218919437No Hit
CTTATACACATCTCCGAGCCCACGAGACGCATGTACATCACGTATGCCGT2350.1101275136019195No Hit
CTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTAGTATGCCGT2140.100286331535365No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGAC150.002223152769.9941545
TAACGTA150.002223152769.9941538
TCTACAG150.002223152769.9941550
TATTCAC150.002223152769.994153
CCGTCAA252.8344748E-669.9941447
CAACTCG252.8344748E-669.9941436
GACGACT252.8344748E-669.9941446
CCTATGC207.91713E-569.9941441
AGGGGGG59750.064.8983470
CTTAAGC401.0622898E-861.24487351
CTTTATA2000.061.2448732
GACGCAA1150.060.87873526
CATGTCC352.9613147E-760.0090430
GCATGTC352.9613147E-760.0090429
CGTCAAC352.966135E-759.9949848
CATCTAG1000.059.4950236
TCTTTAT1950.059.2258071
TGACGAC308.360965E-658.32845345
AAGCCGT1200.058.32845344
AACTCGT308.360965E-658.32845337
Produced by FastQC (version 0.11.5)