FastQCFastQC Report
Wed 25 May 2016
SRR1780132_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780132_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences252818
Sequences flagged as poor quality0
Sequence length76
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9009535.636307541393414No Hit
CTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGTG61042.4143850516972685No Hit
TCTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGT5670.22427200594894353No Hit
ATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGTGGTC4760.18827773338923653No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGG3730.1475369633491286No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCAA150.002222168770.0057430
ACCGTCA150.002223913369.99198
CGGGTGA252.3864911E-456.0045956
GTTCTAT200.006943544452.5043032
GACCCCG200.006943544452.50430320
CGATGTG200.006943544452.50430318
TATAAGC200.006943544452.5043033
CCACCGT200.006943544452.5043036
CCGTCAG200.006948979652.493929
ACGATCA200.006948979652.4939213
CGTCAGA200.006948979652.4939212
CATGTAC352.0818037E-550.0040973
GCCGTAT9950.049.60205554
TCGCCGT10050.049.44700652
TCGGTGG10050.049.44700645
GTCGCCG10200.049.40603651
CTCGGTG9800.049.2800144
TGGTCGC10050.049.0987949
CGGTGGT10200.049.06294346
ATCTCGG10000.049.00401342
Produced by FastQC (version 0.11.5)