FastQCFastQC Report
Wed 25 May 2016
SRR1780132_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780132_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences252818
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGT5926723.44255551424345No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG98123.8810527731411524No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCG74362.9412462720217705No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCC27911.1039562056499141No Hit
ATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGTCTT22220.8788931167875704Illumina PCR Primer Index 3 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCC14510.5739306536718113No Hit
CATATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGT12020.47544083095349227No Hit
CTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGTC9590.3793242569753736No Hit
ATTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGT6400.2531465322880491No Hit
CTTTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCG6270.2480044933509481No Hit
CTTTACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGTC5810.2298095863427446No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGACGT5640.2230853815788433No Hit
CTTAATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCG3910.15465670956973002No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGAAGACATCTCGTATGCCGT3500.13843950984502687No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCAGCCATCTCGTATGCCGT3490.13804396838832678No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGA3460.13685734401822655No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCC3320.13131976362442546No Hit
TTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGTC3190.1261777246873245No Hit
TATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGTCT3100.12261785157702378No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGCCATCTCGTATGCCGT2960.11708027118322271No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGAAGCAATCTCGTATGCCGT2870.11352039807292202No Hit
CCTTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCG2850.11272931515952186No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCAGT2580.10204969582861981No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAACGA150.002222691770.0015923
CCGTCAA150.002222691770.0015947
CAACTCG950.062.63336
AGGGGGG70150.062.5174570
AACTCGT1000.059.50134337
TGCTAGC308.360148E-658.33465660
ACGTATG1200.058.33465640
CACGTAT1200.058.33465639
CGTCTAC850.057.6369648
CATCACG1400.057.501336
CAAATCG252.3871909E-456.00126636
CCTCTCG252.3871909E-456.00126636
CTTAAGC252.3895259E-455.99019251
AGACACG653.6379788E-1253.8473725
AGCCGTC850.053.5306245
AAGCCGT850.053.5306244
CTGCTTG87350.053.20189754
CCGTCTT88250.053.1853447
CGTCTTC89100.053.17811248
GCCGTCT89300.053.10870446
Produced by FastQC (version 0.11.5)