FastQCFastQC Report
Wed 25 May 2016
SRR1780131_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780131_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72310
Sequences flagged as poor quality0
Sequence length76
%GC64

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2327232.18365371317937No Hit
CTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGTG35414.896971373254045No Hit
TCTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGT3740.5172175356105656No Hit
ATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGTGGTC2930.40519983404784954No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGG2420.33467017010095423No Hit
TCTTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGG1320.1825473655096114No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTAGT150.002216458370.011
GACACTC150.002216458370.068
ATAGATC150.002216458370.036
ACAGGAG150.002216458370.015
ATCTTTT150.002216458370.022
ATGACAC150.002216458370.066
GATCTTT150.002216458370.021
CATGGTG200.006925849752.52
GGAGATC200.006925849752.518
GCAAACT200.006925849752.530
CAGGAGA200.006925849752.516
TTAGTTG200.006925849752.513
ATCGGTG200.006925849752.544
CAACTAC200.006925849752.51
AAAGGGG407.386352E-752.570
GAGATCT200.006925849752.519
AAAAAGG2900.047.06896670
TGACACT305.842722E-446.66666467
TCGCCGT5500.043.9090952
GTCGCCG5500.043.9090951
Produced by FastQC (version 0.11.5)