##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780131_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 72310 Sequences flagged as poor quality 0 Sequence length 76 %GC 64 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.194689531185176 32.0 27.0 32.0 22.0 32.0 2 29.586350435624396 32.0 32.0 32.0 22.0 32.0 3 29.356354584428157 32.0 32.0 32.0 22.0 32.0 4 28.547047434656342 32.0 27.0 32.0 13.0 32.0 5 29.187415295256535 32.0 32.0 32.0 22.0 32.0 6 33.27380721891854 37.0 32.0 37.0 22.0 37.0 7 33.3502696722445 37.0 32.0 37.0 22.0 37.0 8 33.01406444475176 37.0 32.0 37.0 13.0 37.0 9 33.39771815793113 37.0 32.0 37.0 22.0 37.0 10-11 33.00937629650117 37.0 32.0 37.0 17.5 37.0 12-13 32.93463559673627 37.0 32.0 37.0 13.0 37.0 14-15 33.137173281703774 37.0 34.5 37.0 17.5 37.0 16-17 33.46863504356244 37.0 34.5 37.0 22.0 37.0 18-19 33.083231918130274 37.0 32.0 37.0 17.5 37.0 20-21 33.25990872631724 37.0 34.5 37.0 17.5 37.0 22-23 33.206914672935966 37.0 34.5 37.0 17.5 37.0 24-25 32.480189462038446 37.0 32.0 37.0 13.0 37.0 26-27 32.839351403678606 37.0 32.0 37.0 17.5 37.0 28-29 32.85610565620246 37.0 32.0 37.0 13.0 37.0 30-31 32.90019361084221 37.0 32.0 37.0 13.0 37.0 32-33 32.76425805559397 37.0 32.0 37.0 13.0 37.0 34-35 32.849066519153645 37.0 32.0 37.0 13.0 37.0 36-37 33.33183515419721 37.0 32.0 37.0 22.0 37.0 38-39 33.3399668095699 37.0 34.5 37.0 22.0 37.0 40-41 32.82255566311713 37.0 32.0 37.0 17.5 37.0 42-43 32.60402433964873 37.0 32.0 37.0 13.0 37.0 44-45 32.20360945927258 37.0 32.0 37.0 13.0 37.0 46-47 32.35948693126815 37.0 32.0 37.0 13.0 37.0 48-49 32.73524408795464 37.0 32.0 37.0 13.0 37.0 50-51 32.95793804453049 37.0 32.0 37.0 13.0 37.0 52-53 32.04791176877333 37.0 32.0 37.0 13.0 37.0 54-55 32.006935416954775 37.0 29.5 37.0 13.0 37.0 56-57 31.709327893790622 37.0 29.5 37.0 13.0 37.0 58-59 31.480811782602682 37.0 27.0 37.0 13.0 37.0 60-61 31.338563130963905 37.0 27.0 37.0 13.0 37.0 62-63 31.075349190983268 37.0 27.0 37.0 13.0 37.0 64-65 31.062508643341168 37.0 27.0 37.0 13.0 37.0 66-67 31.54276033743604 37.0 29.5 37.0 13.0 37.0 68-69 31.816864887290834 37.0 32.0 37.0 13.0 37.0 70-71 31.724865163877748 37.0 32.0 37.0 13.0 37.0 72-73 31.621387083390957 37.0 32.0 37.0 13.0 37.0 74-75 30.882415986723828 37.0 27.0 37.0 13.0 37.0 76 29.858760890609876 37.0 27.0 37.0 13.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 11101 1 0.5380921863944934 11101 2 -0.16954977710922137 11101 3 -1.9332023448022397 11101 4 0.03888576241075725 11101 5 -1.0579763543544942 11101 6 -1.2092873404795093 11101 7 -1.1097957648061652 11101 8 1.0530182189585915 11101 9 -0.46294209406074316 11101 10-11 -0.7931331276346469 11101 12-13 0.5712254840133966 11101 14-15 -1.0855954355727988 11101 16-17 -0.1927480675085249 11101 18-19 -1.1971869973806406 11101 20-21 -1.935925148516965 11101 22-23 -1.121909022219235 11101 24-25 -3.1519095466156344 11101 26-27 -0.16380154959740878 11101 28-29 -1.1182231617755036 11101 30-31 -0.08059718711135844 11101 32-33 0.47552624121359344 11101 34-35 -1.5210840528963416 11101 36-37 -2.4693425937091042 11101 38-39 -0.5270973701219219 11101 40-41 -1.9945759392703835 11101 42-43 -3.144476390924197 11101 44-45 -2.6714210150055493 11101 46-47 -1.243549277986979 11101 48-49 -2.00978704612281 11101 50-51 -0.12916695923760102 11101 52-53 -0.5276078327436942 11101 54-55 -3.7801576417108294 11101 56-57 -3.1576439808168466 11101 58-59 -1.949211812748235 11101 60-61 0.244215126656659 11101 62-63 -1.026735971129039 11101 64-65 -2.3300401617744164 11101 66-67 -2.9995104442661855 11101 68-69 -1.309969525331013 11101 70-71 -3.091851883527241 11101 72-73 -1.1230355543326525 11101 74-75 -1.936863999358735 11101 76 -2.1673009456788073 11102 1 0.5789524014482552 11102 2 0.4637835562241115 11102 3 -0.07298729103879609 11102 4 -1.0105766031806418 11102 5 0.48718493596808443 11102 6 1.8928631971549024 11102 7 1.0461182136884588 11102 8 -2.722250598245708 11102 9 0.9650148951865702 11102 10-11 -0.10388581580668443 11102 12-13 -1.006731505233919 11102 14-15 0.22193144614762872 11102 16-17 0.0782196744269612 11102 18-19 1.1667914972430111 11102 20-21 0.7855802278271256 11102 22-23 0.7495963541248543 11102 24-25 0.2948646469327514 11102 26-27 0.7872737192197974 11102 28-29 -0.15639520478625712 11102 30-31 -0.36285525162748655 11102 32-33 -0.2357640813670514 11102 34-35 -0.3673206120361243 11102 36-37 0.1209799869360566 11102 38-39 0.403010156759791 11102 40-41 0.09467137255757763 11102 42-43 -0.26866993931129457 11102 44-45 -1.1144317676937234 11102 46-47 -2.175269708094504 11102 48-49 0.5386000506513824 11102 50-51 -0.5544357764419061 11102 52-53 -0.410941166077027 11102 54-55 -0.5059640933237333 11102 56-57 -0.6028052711394274 11102 58-59 -0.08630858694178656 11102 60-61 -0.4127741206551683 11102 62-63 -1.8149080141397889 11102 64-65 -0.3983197316668914 11102 66-67 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0.8928846117195022 21612 4 -0.20459249845880834 21612 5 0.711588863036809 21612 6 0.14578512328861137 21612 7 1.245276698961952 21612 8 2.237076189973088 21612 9 0.12256515231607068 21612 10-11 0.9865770172928876 21612 12-13 1.408182005752522 21612 14-15 0.3825205064561885 21612 16-17 1.692759178868286 21612 18-19 0.8107840171121126 21612 20-21 0.4090023877149207 21612 22-23 0.48896054299815006 21612 24-25 0.42997451135538256 21612 26-27 0.44272018953302705 21612 28-29 0.02453046141290116 21612 30-31 -0.917553708850491 21612 32-33 0.21538131367736213 21612 34-35 0.8977565268138008 21612 36-37 0.9031211744068415 21612 38-39 0.40768523857371974 21612 40-41 -0.3510976784008193 21612 42-43 0.6866830293656605 21612 44-45 -0.020696377324391335 21612 46-47 0.21369709882461052 21612 48-49 0.8815173017032762 21612 50-51 1.3903982581537022 21612 52-53 2.118044341169348 21612 54-55 1.3357843872746784 21612 56-57 1.0930806568643092 21612 58-59 0.4747012307300267 21612 60-61 -0.01375588783609416 21612 62-63 1.6413799708999406 21612 64-65 0.9301047657618149 21612 66-67 1.4178808600816453 21612 68-69 0.9045232282921738 21612 70-71 -0.5143156516431837 21612 72-73 0.8740658949427065 21612 74-75 0.4037157107861944 21612 76 -0.5571560181425745 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 3.0 11 7.0 12 23.0 13 50.0 14 83.0 15 141.0 16 220.0 17 304.0 18 418.0 19 557.0 20 628.0 21 851.0 22 908.0 23 1143.0 24 1376.0 25 1622.0 26 1819.0 27 2238.0 28 2585.0 29 3169.0 30 3716.0 31 4303.0 32 5279.0 33 6524.0 34 8437.0 35 9662.0 36 16243.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 56.567556354584426 13.794772507260406 7.8467708477389015 21.790900290416264 2 45.694924630064996 13.367445719817454 27.31710690084359 13.620522749273958 3 45.11962384179228 15.010372009403955 25.1860047019776 14.683999446826165 4 42.67241975411083 17.766806344991632 22.93075550761316 16.630018393284377 5 42.07578481537823 21.499101092518323 28.30037339233854 8.124740699764901 6 50.401748053492646 28.79724515620462 12.057973419629645 8.743033370673084 7 48.874291246024065 19.712349605863643 14.309224173696583 17.10413497441571 8 48.06250864334117 27.94219333425529 12.718849398423455 11.276448623980086 9 48.06527451251556 11.268151016456923 12.843313511270917 27.823260959756603 10-11 49.1896003319043 18.963490526898077 19.172313649564376 12.674595491633244 12-13 48.409625224726874 15.319457889641818 18.849398423454574 17.42151846217674 14-15 51.86212142165676 17.91453464251141 14.285714285714285 15.937629650117549 16-17 51.544737933895725 17.133868068040382 14.603097773475316 16.718296224588578 18-19 48.01925084879371 16.132266607660235 18.505431588264173 17.343050955281882 20-21 51.43137688775793 16.36817502904243 15.588178348177243 16.6122697350224 22-23 51.724185618763784 15.911463915529772 14.908137934849503 17.45621253085694 24-25 48.868068040381694 19.48623980085742 15.065689392891715 16.580002765869175 26-27 51.70620734481638 19.319038300638226 15.157067881813594 13.817686472731799 28-29 54.84102947074362 17.051819275608846 15.743545063546724 12.363606190100818 30-31 49.26254503211888 18.954632517165795 15.191641485558605 16.59118096515672 32-33 47.69771188726084 20.536313158204084 15.08190599998617 16.684068954548913 34-35 48.27028260463701 16.17560624814167 18.691873128703698 16.862238018517623 36-37 51.47697413912322 15.407274235928641 19.64804314755912 13.46770847738902 38-39 51.52923279051274 16.021159630743696 19.486222037824568 12.963385540918992 40-41 50.909631514531284 18.761020336193724 17.036488981392488 13.292859167882506 42-43 48.1496335223344 19.036094592725764 19.598257502420136 13.216014382519706 44-45 47.519015350573916 15.948693126815103 19.824367307426357 16.707924215184622 46-47 50.6696999661174 15.108182302218964 17.087894230968697 17.13422350069494 48-49 51.64845802793528 14.87069561609736 19.83543078412391 13.645415571843452 50-51 53.96285386328119 15.016802887607353 17.35606909236748 13.664274156743975 52-53 47.53936259222636 15.560411293278106 20.1055200979138 16.794706016581728 54-55 49.831971123926486 15.45796512190737 17.064957335877967 17.64510641828818 56-57 49.52841930576683 15.791730051168578 16.752178121974833 17.92767252108975 58-59 47.07090403684189 18.745937573469416 19.734749477934972 14.448408911753724 60-61 46.29671887425232 16.586799432977216 20.334681741174844 16.781799951595616 62-63 46.262835805414376 19.175742488676832 19.68744597724994 14.873975728658854 64-65 46.709907617414395 19.20534380704763 19.8394368534602 14.245311722077778 66-67 46.60240497313593 23.023572609029365 15.79482356846014 14.579198849374556 68-69 46.679528136193284 23.138198564494047 15.38121119086144 14.80106210845123 70-71 47.37580903911047 23.562399734469214 14.692011948885325 14.369779277534988 72-73 48.06522099905958 22.626818609282516 14.476959672512033 14.831000719145877 74-75 50.306680588882045 20.890929584477192 14.067891545020158 14.734498281620601 76 55.48271999336182 14.959410308536974 14.285911850530365 15.27195784757084 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.5 2 1.0 3 1.0 4 1.0 5 3.5 6 5.5 7 3.0 8 1.0 9 1.5 10 3.0 11 5.0 12 6.0 13 4.0 14 6.0 15 15.5 16 23.5 17 26.0 18 34.0 19 52.5 20 65.0 21 67.0 22 75.0 23 104.5 24 152.5 25 179.0 26 243.0 27 320.0 28 378.5 29 424.0 30 512.0 31 638.5 32 799.0 33 921.0 34 1024.5 35 1259.5 36 1492.0 37 1593.0 38 1735.5 39 2086.5 40 2314.5 41 2372.0 42 2410.0 43 2543.5 44 2936.5 45 4078.0 46 4960.0 47 4760.5 48 3554.5 49 2324.5 50 2101.0 51 1911.5 52 1554.5 53 1250.5 54 1114.0 55 1068.0 56 873.0 57 637.0 58 550.0 59 478.0 60 376.0 61 325.5 62 305.0 63 285.5 64 229.0 65 187.5 66 175.5 67 168.0 68 159.0 69 140.0 70 143.0 71 156.0 72 120.5 73 90.0 74 89.0 75 83.0 76 71.5 77 55.0 78 58.5 79 67.0 80 66.0 81 60.5 82 49.5 83 43.0 84 42.5 85 43.5 86 48.5 87 52.0 88 59.0 89 79.0 90 91.0 91 94.0 92 98.0 93 129.5 94 173.0 95 220.0 96 255.0 97 318.0 98 593.0 99 12219.0 100 23633.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0013829345871940258 5 0.0 6 0.0013829345871940258 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0020744018807910387 20-21 0.0027658691743880515 22-23 6.914672935970129E-4 24-25 0.0 26-27 6.914672935970129E-4 28-29 0.0013829345871940258 30-31 6.914672935970129E-4 32-33 0.0020744018807910387 34-35 6.914672935970129E-4 36-37 0.0 38-39 0.0034573364679850644 40-41 6.914672935970129E-4 42-43 0.0 44-45 0.0 46-47 0.0020744018807910387 48-49 0.0 50-51 0.0013829345871940258 52-53 0.0020744018807910387 54-55 0.0013829345871940258 56-57 0.0 58-59 0.0013829345871940258 60-61 0.0034573364679850644 62-63 0.0034573364679850644 64-65 0.0027658691743880515 66-67 0.0020744018807910387 68-69 0.0013829345871940258 70-71 0.0027658691743880515 72-73 0.0027658691743880515 74-75 0.004840271055179091 76 0.0013829345871940258 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 72310.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.49993085327064 #Duplication Level Percentage of deduplicated Percentage of total 1 69.27541877678223 24.59272576407136 2 14.051421893260615 9.976490112017702 3 7.580833657966497 8.073572120038722 4 3.7397740553174916 5.310468814825059 5 2.0724581223217764 3.6786060019361084 6 1.184261784183872 2.522472687041903 7 0.6895208414491624 1.713455953533398 8 0.436306973120374 1.2391093901258472 9 0.2493182703544994 0.7965703222237589 >10 0.6934164394234515 3.5002074401880794 >50 0.0038955979742890533 0.07606140229567142 >100 0.015582391897156213 1.4396349052689807 >500 0.0 0.0 >1k 0.0038955979742890533 4.896971373254045 >5k 0.0 0.0 >10k+ 0.0038955979742890533 32.18365371317937 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 23272 32.18365371317937 No Hit CTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGTG 3541 4.896971373254045 No Hit TCTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGT 374 0.5172175356105656 No Hit ATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGTGGTC 293 0.40519983404784954 No Hit CTCTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGG 242 0.33467017010095423 No Hit TCTTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGG 132 0.1825473655096114 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0027658691743880515 0.0 2 0.0 0.0 0.0 0.004148803761582077 0.0 3 0.0 0.0 0.0 0.008297607523164155 0.0 4 0.0 0.0 0.0 0.013829345871940258 0.0 5 0.0 0.0 0.0 0.01659521504632831 0.0 6 0.0 0.0 0.0 0.01936108422071636 0.0 7 0.0 0.0 0.0 0.01936108422071636 0.0 8 0.0 0.0 0.0 0.01936108422071636 0.0 9 0.0 0.0 0.0 0.023509887982298437 0.0 10 0.0 0.0 0.0 0.023509887982298437 0.0 11 0.0 0.0 0.0 0.023509887982298437 0.0 12 0.0 0.0 0.0 0.02904162633107454 0.0 13 0.0 0.0 0.0 0.03733923385423869 0.0 14 0.0 0.0 0.0 0.03733923385423869 0.0 15 0.0 0.0 0.0 0.03733923385423869 0.0 16 0.0 0.0 0.0 0.03733923385423869 0.0 17 0.0 0.0 0.0 0.03733923385423869 0.0 18 0.0 0.0 0.0 0.04010510302862674 0.0 19 0.0 0.0 0.0 0.04563684137740285 0.0 20 0.0 0.0 0.0 0.05808325266214908 0.0 21 0.0 0.0 0.0 0.06084912183653713 0.0 22 0.0 0.0 0.0 0.09818835569077583 0.0 23 0.0 0.0 0.0 0.10925183238832803 0.0 24 0.0 0.0 0.0 0.12169824367307426 0.0 25 0.0 0.0 0.0 0.12169824367307426 0.0 26 0.0 0.0 0.0 0.12584704743465633 0.0 27 0.0 0.0 0.0 0.13691052413220856 0.0 28 0.0 0.0 0.0 0.15212280459134284 0.0 29 0.0 0.0 0.0 0.15903747752731295 0.0 30 0.0 0.0 0.0 0.20190844973032776 0.0 31 0.0 0.0 0.0 0.22265246853823814 0.0 32 0.0 0.0 0.0 0.2572258332180888 0.0 33 0.0 0.0 0.0 0.2945650670723275 0.0 34 0.0 0.0 0.0 0.3540312543216706 0.0 35 0.0 0.0 0.0 0.44945374083805834 0.0 36 0.0 0.0 0.0 0.5739178536855207 0.0 37 0.0 0.0 0.0 0.802102060572535 0.0 38 0.0 0.0 0.0 1.075923108836952 0.0 39 0.0 0.0 0.0 1.4119762135251002 0.0 40 0.0 0.0 0.0 1.774305075369935 0.0 41 0.0 0.0 0.0 2.216844143272023 0.0 42 0.0 0.0 0.0 2.7147005946618723 0.0 43 0.0 0.0 0.0 3.272023233301065 0.0 44 0.0 0.0 0.0 3.7380721891854516 0.0 45 0.0 0.0 0.0 4.336882865440464 0.0 46 0.0 0.0 0.0 4.895588438666851 0.0 47 0.0 0.0 0.0 5.487484441985894 0.0 48 0.0 0.0 0.0 6.021297192642788 0.0 49 0.0 0.0 0.0 6.628405476420966 0.0 50 0.0 0.0 0.0 7.184345180472963 0.0 51 0.0 0.0 0.0 7.7485824920481265 0.0 52 0.0 0.0 0.0 8.290692850228185 0.0 53 0.0 0.0 0.0 8.748444198589407 0.0 54 0.0 0.0 0.0 9.246300649979256 0.0 55 0.0 0.0 0.0 9.775964596874568 0.0 56 0.0 0.0 0.0 10.242013552758955 0.0 57 0.0 0.0 0.0 10.692850228184207 0.0 58 0.0 0.0 0.0 11.207301894620384 0.0 59 0.0 0.0 0.0 11.671967915917577 0.0 60 0.0 0.0 0.0 12.146314479325127 0.0 61 0.0 0.0 0.0 12.554280182547366 0.0 62 0.0 0.0 0.0 13.068731848983543 0.0 63 0.0 0.0 0.0 13.497441571013692 0.0 64 0.0 0.0 0.0 13.897109666712764 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATTTAGT 15 0.0022164583 70.0 11 GACACTC 15 0.0022164583 70.0 68 ATAGATC 15 0.0022164583 70.0 36 ACAGGAG 15 0.0022164583 70.0 15 ATCTTTT 15 0.0022164583 70.0 22 ATGACAC 15 0.0022164583 70.0 66 GATCTTT 15 0.0022164583 70.0 21 CATGGTG 20 0.0069258497 52.5 2 GGAGATC 20 0.0069258497 52.5 18 GCAAACT 20 0.0069258497 52.5 30 CAGGAGA 20 0.0069258497 52.5 16 TTAGTTG 20 0.0069258497 52.5 13 ATCGGTG 20 0.0069258497 52.5 44 CAACTAC 20 0.0069258497 52.5 1 AAAGGGG 40 7.386352E-7 52.5 70 GAGATCT 20 0.0069258497 52.5 19 AAAAAGG 290 0.0 47.068966 70 TGACACT 30 5.842722E-4 46.666664 67 TCGCCGT 550 0.0 43.90909 52 GTCGCCG 550 0.0 43.90909 51 >>END_MODULE