FastQCFastQC Report
Wed 25 May 2016
SRR1780131_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780131_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72310
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGT771510.66934034020191No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG59928.286544046466602No Hit
TCTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCG24443.3798921311021988No Hit
ATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGTCTT14291.9762135251002628TruSeq Adapter, Index 3 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCC7471.0330521366339374No Hit
CTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGTC6550.9058221546120869TruSeq Adapter, Index 3 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGTC4270.5905130687318491RNA PCR Primer, Index 3 (95% over 21bp)
ATTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGT3900.53934448900567No Hit
CATATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGT3060.4231779836813719No Hit
CTTATACACATCTCCGAGCCCACGAGACAACTAGGCATCTCGTATGCCGT2750.38030701147835705No Hit
TATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGTCT2580.35679712349605863RNA PCR Primer, Index 3 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCC2490.3443507122113124No Hit
CTTAATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCG2330.322223758816208No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCC2320.32084082422901394No Hit
CTTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCG1930.26690637532844697No Hit
TTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGTC1810.2503111602821187TruSeq Adapter, Index 3 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGACGT1600.22126953395104412No Hit
CTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGA1540.21297192642788No Hit
ATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGTCAT1290.17839856174802932Illumina PCR Primer Index 3 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCACGTATGCCGT950.13137878578343246No Hit
TCTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGT910.12584704743465633No Hit
CTTATACACATCTCCGAGCCCACGAGACCACAAGGCATCTCGTATGCCGT900.12446411284746232No Hit
TCTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGACG840.11616650532429815No Hit
TCTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCAG740.1023371594523579No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCATA207.878553E-570.040
GCCCAGC150.002216458370.06
CTCGTCC150.002216458370.061
TTTACCG207.878553E-570.026
AGCCCGG150.002216458370.030
TTAGAGC150.002216458370.032
GGACTAC150.002216458370.010
AAGGTCG150.002216458370.039
TCTCGTC150.002216458370.060
CTTCTCG150.002216458370.058
CGTAAGA207.878553E-570.041
CGGCTCG150.002216458370.014
CCGGTAA207.878553E-570.033
AGCTCGG150.002216458370.010
CGGTAAT207.878553E-570.034
AAAGGGC150.002216458370.069
ATGCCCT150.002216458370.044
TTACCGT207.878553E-570.027
CCCGGTA207.878553E-570.032
ACGAGAG150.002216458370.05
Produced by FastQC (version 0.11.5)