##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780131_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 72310 Sequences flagged as poor quality 0 Sequence length 76 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.947711243258194 32.0 27.0 32.0 13.0 32.0 2 27.775867791453464 32.0 27.0 32.0 13.0 32.0 3 28.333964873461486 32.0 27.0 32.0 13.0 32.0 4 30.113372977458166 32.0 32.0 32.0 27.0 32.0 5 29.519914258055593 32.0 32.0 32.0 22.0 32.0 6 32.335541418890884 37.0 32.0 37.0 13.0 37.0 7 33.900732955331215 37.0 37.0 37.0 27.0 37.0 8 32.9136495643756 37.0 32.0 37.0 22.0 37.0 9 34.09164707509335 37.0 37.0 37.0 27.0 37.0 10-11 33.80920342967778 37.0 34.5 37.0 24.5 37.0 12-13 33.86712764486239 37.0 37.0 37.0 24.5 37.0 14-15 33.86567556354585 37.0 37.0 37.0 24.5 37.0 16-17 32.84865855345042 37.0 32.0 37.0 22.0 37.0 18-19 33.11855206748721 37.0 32.0 37.0 22.0 37.0 20-21 33.72756880099571 37.0 34.5 37.0 24.5 37.0 22-23 33.21340063614991 37.0 32.0 37.0 22.0 37.0 24-25 33.00468123357766 37.0 32.0 37.0 17.5 37.0 26-27 33.11576545429401 37.0 32.0 37.0 22.0 37.0 28-29 32.992974692297054 37.0 32.0 37.0 17.5 37.0 30-31 33.00422486516388 37.0 32.0 37.0 17.5 37.0 32-33 32.47658000276587 37.0 32.0 37.0 13.0 37.0 34-35 31.894143272023236 37.0 32.0 37.0 13.0 37.0 36-37 32.497254874844415 37.0 32.0 37.0 13.0 37.0 38-39 31.349972341308256 37.0 27.0 37.0 13.0 37.0 40-41 31.381199004287097 37.0 29.5 37.0 13.0 37.0 42-43 31.648340478495367 37.0 32.0 37.0 13.0 37.0 44-45 32.27557737519015 37.0 32.0 37.0 13.0 37.0 46-47 31.055822154612088 37.0 27.0 37.0 13.0 37.0 48-49 30.22673212557046 37.0 24.5 37.0 10.5 37.0 50-51 30.186537131793667 37.0 27.0 37.0 13.0 37.0 52-53 30.10412114506984 37.0 27.0 37.0 13.0 37.0 54-55 30.00407965703222 37.0 24.5 37.0 13.0 37.0 56-57 29.10341584843037 37.0 22.0 37.0 10.5 37.0 58-59 29.858180058083253 37.0 24.5 37.0 13.0 37.0 60-61 29.810973585949384 37.0 24.5 37.0 13.0 37.0 62-63 31.11745954916332 37.0 27.0 37.0 13.0 37.0 64-65 31.318572811506016 37.0 27.0 37.0 13.0 37.0 66-67 31.095270363711798 37.0 27.0 37.0 13.0 37.0 68-69 30.28368828654405 37.0 27.0 37.0 13.0 37.0 70-71 27.7405614714424 32.0 17.5 37.0 10.5 37.0 72-73 27.1006154058913 32.0 13.0 37.0 8.0 37.0 74-75 28.60033881897386 37.0 17.5 37.0 10.5 37.0 76 29.424298160697 37.0 22.0 37.0 13.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 11101 1 1.35210238074772 11101 2 -2.958705449568498 11101 3 0.029755803270848702 11101 4 0.35782862294286844 11101 5 -0.45854855848499554 11101 6 0.3784241227265568 11101 7 -1.2694777899062402 11101 8 0.11629226494859068 11101 9 0.31447523511683073 11101 10-11 -1.6600978768468764 11101 12-13 -0.2576457527269298 11101 14-15 -1.0862111716537157 11101 16-17 0.6066900897824112 11101 18-19 -2.9290175997171843 11101 20-21 -0.5382352021374146 11101 22-23 -0.38630800017831035 11101 24-25 -1.0734955843415293 11101 26-27 -0.7550800960939696 11101 28-29 -1.3765648624111435 11101 30-31 -1.852281723152732 11101 32-33 0.21601163090167574 11101 34-35 -1.0002899618839791 11101 36-37 0.6832187393117053 11101 38-39 -0.1896578486040319 11101 40-41 -2.196045484023724 11101 42-43 0.12217460973109695 11101 44-45 -0.3477395043970759 11101 46-47 -1.6532090555658812 11101 48-49 -0.8511469540423136 11101 50-51 1.0561329790482539 11101 52-53 1.3941492008621026 11101 54-55 0.34232956634099665 11101 56-57 0.5289418645991191 11101 58-59 0.5625466863706805 11101 60-61 -2.648646174249066 11101 62-63 -0.6872247781720162 11101 64-65 -1.2812477332646992 11101 66-67 -0.6026289243441241 11101 68-69 -1.5021136084420874 11101 70-71 -1.029794854247445 11101 72-73 2.310327729381047 11101 74-75 0.29924096467591355 11101 76 -1.1501131683603525 11102 1 0.02307012268320463 11102 2 1.468176270861612 11102 3 -0.4917495730732391 11102 4 -1.1755047103904666 11102 5 -4.520914149882845 11102 6 0.03756390767279072 11102 7 -1.1275423060352736 11102 8 -1.590159347954632 11102 9 -1.8263849799369325 11102 10-11 0.5006548113251625 11102 12-13 -0.7248500538022 11102 14-15 -0.08889934369672403 11102 16-17 1.3351847134383235 11102 18-19 -1.4177272771365388 11102 20-21 -0.25382659998687984 11102 22-23 0.47659522562813805 11102 24-25 0.36575172748642615 11102 26-27 -0.48948869824450725 11102 28-29 -0.9566723892928621 11102 30-31 -1.4474430134753113 11102 32-33 0.5036460395038276 11102 34-35 -2.856203940378599 11102 36-37 1.9278423952256745 11102 38-39 -3.307937418496504 11102 40-41 -2.3245401076796384 11102 42-43 1.6528197710214165 11102 44-45 -2.3568792893433113 11102 46-47 -2.9671875501895357 11102 48-49 -0.051684588450918056 11102 50-51 -1.6680605693388415 11102 52-53 -1.5332701539766056 11102 54-55 -0.4372403261321196 11102 56-57 -3.119982866583676 11102 58-59 -1.545517829758353 11102 60-61 -1.4104741312383133 11102 62-63 1.0756784476344308 11102 64-65 -1.6183445074582465 11102 66-67 -0.19510204262369513 11102 68-69 0.02906918725683738 11102 70-71 -0.5991496929571198 11102 72-73 1.236671815402552 11102 74-75 -0.017425701990752884 11102 76 0.0724674768009379 11103 1 -1.7890740898405149 11103 2 -1.1077250574116349 11103 3 -0.5192638045722902 11103 4 -0.7637400045081151 11103 5 -1.0977642447595066 11103 6 1.0470515737069448 11103 7 -0.19300720167094454 11103 8 -0.6072371468161109 11103 9 -0.9345443727263074 11103 10-11 -0.0591174846900131 11103 12-13 0.5384326786456199 11103 14-15 1.6539849067776657 11103 16-17 0.719435187821631 11103 18-19 1.332747106165165 11103 20-21 0.36666675864689324 11103 22-23 -0.4451315295900784 11103 24-25 -0.28035832943956507 11103 26-27 -0.4629232333488673 11103 28-29 -0.3746040780974198 11103 30-31 0.5506594533178557 11103 32-33 1.059148885803637 11103 34-35 -0.4600938834526076 11103 36-37 0.6930226608803238 11103 38-39 -0.13573627997658022 11103 40-41 1.4382682414664707 11103 42-43 0.8515863744369803 11103 44-45 2.2071624563872447 11103 46-47 3.0252223169831396 11103 48-49 1.814539320467489 11103 50-51 0.6061329790482546 11103 52-53 1.9823844949797476 11103 54-55 0.9991923114390389 11103 56-57 1.529922256755981 11103 58-59 0.3890172746059726 11103 60-61 0.9993930414372088 11103 62-63 -0.5666365428779017 11103 64-65 0.16581109026471097 11103 66-67 0.2218808795774443 11103 68-69 -0.10897635354012536 11103 70-71 -0.4592066189533277 11103 72-73 -0.03182913336405235 11103 74-75 1.80610370977395 11103 76 0.7204750669337621 11104 1 -1.0017437730984327 11104 2 -0.9048592957223427 11104 3 0.8630891366041809 11104 4 0.7988542639685079 11104 5 -0.36624086617730356 11104 6 0.4271420714445 11104 7 1.6177016972732474 11104 8 -1.9119128632565356 11104 9 1.4349880556296526 11104 10-11 -1.856251723000721 11104 12-13 -0.2666201117012861 11104 14-15 -1.990057325499869 11104 16-17 1.2925875256798491 11104 18-19 -0.2969663176658983 11104 20-21 -0.6061839200861385 11104 22-23 0.530358666488361 11104 24-25 -0.5722135330594753 11104 26-27 1.4013301603162844 11104 28-29 0.4349735991273178 11104 30-31 -1.4433073641783736 11104 32-33 0.6198577847478361 11104 34-35 1.774069012474996 11104 36-37 -0.29755049145753176 11104 38-39 0.07957292062673815 11104 40-41 0.705236567258325 11104 42-43 0.200379737936224 11104 44-45 -1.413124119781692 11104 46-47 0.6006370982802736 11104 48-49 0.21423766134229894 11104 50-51 -0.9836106106953366 11104 52-53 -1.4712354145225106 11104 54-55 -0.4397217157102844 11104 56-57 -0.7543914687342124 11104 58-59 -1.851555877731883 11104 60-61 -1.0986461742490654 11104 62-63 -0.6910709320181709 11104 64-65 -1.1158631178800889 11104 66-67 -1.5205776422928423 11104 68-69 -1.0482674545959298 11104 70-71 0.884307709855122 11104 72-73 -1.6653132962599813 11104 74-75 0.8671896826246339 11104 76 -1.3270362452834306 11105 1 1.2331834618288013 11105 2 1.382735991872945 11105 3 -0.02880275528771037 11105 4 0.09476555987980362 11105 5 -0.28377378371022033 11105 6 0.43337907768150785 11105 7 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11105 68-69 -1.184095590424068 11105 70-71 0.4553402808876932 11105 72-73 0.594561963615277 11105 74-75 -0.7471554317204827 11105 76 -0.4122753305225153 11106 1 1.6975569262022674 11106 2 -2.2708266616897106 11106 3 1.1130891366041809 11106 4 -0.3800501649359198 11106 5 -0.05097280090923917 11106 6 -0.27915163484920313 11106 7 -0.1876596080880617 11106 8 1.1253831740394986 11106 9 0.9478085684501636 11106 10-11 0.7777809110319112 11106 12-13 -0.547797267878444 11106 14-15 -0.3286354140779508 11106 16-17 0.20138705947938718 11106 18-19 -0.18129032698990954 11106 20-21 -0.17611399001620498 11106 22-23 0.07960109073078314 11106 24-25 1.1568074459614976 11106 26-27 0.7282532372393646 11106 28-29 -1.0417163775626577 11106 30-31 -0.10909990497091115 11106 32-33 1.3485873884774406 11106 34-35 1.3300130684190492 11106 36-37 0.8347338908268469 11106 38-39 1.7004936665474908 11106 40-41 1.331984819006582 11106 42-43 0.09490188245836961 11106 44-45 1.1530180713604992 11106 46-47 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21611 16-17 -0.4484381153457946 21611 18-19 -0.8328637535633376 21611 20-21 -1.0369531508553678 21611 22-23 -0.09015415402446791 21611 24-25 0.4662480054020577 21611 26-27 0.9782532372393646 21611 28-29 0.31702488117859673 21611 30-31 -0.2484355693065794 21611 32-33 0.38652445141450187 21611 34-35 0.027915166321150764 21611 36-37 0.8280905341834952 21611 38-39 -0.17170913065531224 21611 40-41 1.0898519518737153 21611 42-43 0.9798669174234043 21611 44-45 0.0689271622695955 21611 46-47 -0.16346546582229138 21611 48-49 0.15526330236794195 21611 50-51 1.3215175944328692 21611 52-53 -0.10969695298405213 21611 54-55 0.22950905352048423 21611 56-57 2.0353521210093746 21611 58-59 2.2971620709860687 21611 60-61 -0.4653128409157361 21611 62-63 -0.5372247781720176 21611 64-65 0.12259842058145409 21611 66-67 -1.6923725140877153 21611 68-69 -0.28160078792926413 21611 70-71 -0.7259487004012897 21611 72-73 0.5962251652784829 21611 74-75 -1.5097333942984434 21611 76 -1.06549778374497 21612 1 -0.36963674968706073 21612 2 1.8978162895619377 21612 3 0.5732340641404114 21612 4 -0.0015916669122049143 21612 5 0.9124659342686279 21612 6 1.768279195190324 21612 7 0.5218265579198444 21612 8 1.2452777721949673 21612 9 -0.2985682431440395 21612 10-11 0.17106154344297408 21612 12-13 0.9256875806064002 21612 14-15 0.9463975239984634 21612 16-17 1.190023423115747 21612 18-19 -0.14423499102153414 21612 20-21 0.6914749427901228 21612 22-23 0.9434021447492285 21612 24-25 1.0866493431947006 21612 26-27 0.891296715500232 21612 28-29 1.0603916593279905 21612 30-31 0.15424001597770598 21612 32-33 0.20514206568428506 21612 34-35 1.7127535163768925 21612 36-37 1.4549578697464867 21612 38-39 0.28135664414959294 21612 40-41 -0.46923388982082415 21612 42-43 0.30405866770211176 21612 44-45 0.5500865825594516 21612 46-47 0.13519674153556593 21612 48-49 -0.7779585482452163 21612 50-51 0.18584312397578984 21612 52-53 -0.3486044223263036 21612 54-55 1.5017498561960743 21612 56-57 -0.022507410763200397 21612 58-59 1.1980539327474915 21612 60-61 -0.41748675395921 21612 62-63 -0.7473697057082482 21612 64-65 1.3991870493439933 21612 66-67 0.523458032177615 21612 68-69 0.8703501596738548 21612 70-71 0.47237905879603304 21612 72-73 0.8603277293810443 21612 74-75 0.8666322690237394 21612 76 1.0977129185961694 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 2.0 17 8.0 18 24.0 19 59.0 20 158.0 21 320.0 22 727.0 23 1152.0 24 1597.0 25 2111.0 26 2703.0 27 3334.0 28 4037.0 29 5053.0 30 6320.0 31 7846.0 32 9644.0 33 10779.0 34 9199.0 35 5065.0 36 2172.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 31.47092991093655 23.312773137135586 15.702273607346351 29.514023344581513 2 22.922025834647194 19.287472685530936 36.87384172821066 20.916659751611206 3 22.973627072701877 23.20319738898339 39.9189589124452 13.90421662586953 4 17.823260959756603 30.954224865163877 34.83335638224312 16.389157792836397 5 17.92144931544738 36.86212142165676 38.76780528281012 6.448623980085742 6 25.406594014493557 45.501189356641035 17.565138020689275 11.527078608176135 7 23.6343520951459 30.434241460378924 16.495643756050338 29.435762688424838 8 22.292905545567695 44.88175909279491 14.069976490112019 18.755358871525377 9 23.068731848983543 19.80777209238003 13.154473793389574 43.969022265246856 10-11 23.16415433549993 30.480569769049925 27.625501313787858 18.729774581662287 12-13 24.235237173281703 16.508090167335084 30.16871801963767 29.08795463974554 14-15 24.61139538099848 17.144240077444337 18.077720923800307 40.16664361775688 16-17 36.1810261374637 27.642787996127783 14.314064444751764 21.862121421656756 18-19 36.170963102284674 22.258947834264536 15.288073242241524 26.28201582120927 20-21 24.37007329553312 18.42345457059881 14.410869865855345 42.79560226801272 22-23 24.82436730742636 31.33384040934864 14.181302724381137 29.660489558843867 24-25 36.009653283914425 30.559973446554277 15.01237777808511 18.417995491446195 26-27 35.948932874116494 33.69711053017414 14.829799300109272 15.524157295600094 28-29 23.080061686986948 22.947282522250884 17.111223297210945 36.86143249355122 30-31 23.11176576352295 30.575494792747186 16.791142829481487 29.52159661424837 32-33 24.43222683264177 29.616874135546333 27.601659751037346 18.34923928077455 34-35 45.3288800376179 22.789948275385168 18.337344065499405 13.543827621497526 36-37 28.010648596321392 27.496196929885215 17.940810399668095 26.552344074125294 38-39 22.424284331351128 22.1062093762965 29.132208546535747 26.337297745816624 40-41 22.426668879392604 18.41541163617254 31.251987995961777 27.90593148847308 42-43 35.57164391707809 17.404472472306352 29.76807866240717 17.255804948208382 44-45 23.608076337989214 29.468261651223898 31.280597427741668 15.643064583045222 46-47 35.19177977997359 17.8759360803214 21.99987553502652 24.9324086046785 48-49 32.69049923938598 15.973585949384594 19.34310607108284 31.99280874014659 50-51 24.46964458581109 17.0833909556078 31.75425252385562 26.692711934725487 52-53 21.2965011754944 18.022403540312542 42.060572534919096 18.62052274927396 54-55 20.95065552912541 18.47029374343088 33.450655529125406 27.128395198318305 56-57 33.09431613884663 17.505877471995575 23.49744157101369 25.902364818144104 58-59 22.033136487477005 21.869943435628638 38.03988548826531 18.05703458862904 60-61 32.12487899322362 32.43811367722307 21.63393721476974 13.80307011478357 62-63 20.47753721899915 49.780454580028625 15.422114965737086 14.319893235235137 64-65 19.310850338489622 52.034740998388806 14.486249507305708 14.168159155815868 66-67 19.446807039380424 51.579020156968504 14.51785776026 14.456315043391074 68-69 21.955469506292353 49.19858940672106 14.430922417369658 14.415018669616927 70-71 30.692850228184206 39.99377679435763 14.192366201078688 15.121006776379478 72-73 50.85915405306357 19.61291393247084 13.946300278663246 15.58163173580235 74-75 54.077116698475926 16.824329931126048 13.562498271236134 15.536055099161894 76 54.93997897881285 16.12961221441611 13.540687060906123 15.389721745864913 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 1.0 11 2.5 12 4.0 13 7.5 14 9.0 15 8.5 16 22.0 17 34.0 18 38.5 19 55.0 20 73.0 21 79.0 22 92.5 23 130.0 24 197.5 25 241.0 26 277.5 27 359.5 28 448.0 29 491.0 30 575.0 31 722.5 32 857.0 33 928.0 34 1078.0 35 1329.5 36 1522.5 37 1614.0 38 1780.5 39 2169.5 40 2428.0 41 2646.5 42 2829.0 43 2927.0 44 3271.5 45 3840.5 46 4163.0 47 5155.0 48 7660.5 49 9062.5 50 8951.0 51 6164.0 52 3136.0 53 2062.5 54 1230.0 55 1191.5 56 974.0 57 691.5 58 588.0 59 526.0 60 400.5 61 311.0 62 285.0 63 256.0 64 222.5 65 208.5 66 174.0 67 149.0 68 153.0 69 146.0 70 117.5 71 100.0 72 97.5 73 92.5 74 75.0 75 60.0 76 64.0 77 66.0 78 50.0 79 36.0 80 31.0 81 25.5 82 23.5 83 22.0 84 17.0 85 9.5 86 6.0 87 5.0 88 7.5 89 8.0 90 6.5 91 10.0 92 13.0 93 13.5 94 15.5 95 26.5 96 36.0 97 49.5 98 149.0 99 3050.5 100 5866.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0027658691743880515 2 0.005531738348776103 3 0.0013829345871940258 4 0.0 5 0.0 6 0.0027658691743880515 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0027658691743880515 20-21 0.0 22-23 0.0 24-25 0.004148803761582077 26-27 0.017978149633522334 28-29 0.013137878578343243 30-31 0.009680542110358181 32-33 0.013829345871940258 34-35 0.005531738348776103 36-37 0.0 38-39 0.0 40-41 0.0013829345871940258 42-43 0.0013829345871940258 44-45 0.0 46-47 6.914672935970129E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0027658691743880515 56-57 0.0 58-59 0.004148803761582077 60-61 0.0 62-63 0.0020744018807910387 64-65 0.004840271055179091 66-67 0.0034573364679850644 68-69 0.0 70-71 0.0 72-73 6.914672935970129E-4 74-75 0.005531738348776103 76 0.0027658691743880515 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 72310.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.841653989766286 #Duplication Level Percentage of deduplicated Percentage of total 1 77.71472629144179 34.848568662702256 2 11.407864302235929 10.230950076061402 3 5.070161912104857 6.820633384040935 4 2.325366229760987 4.170930714977182 5 1.2243639167309175 2.745125155580141 6 0.7278334618350039 1.9582353754667405 7 0.38858905165767155 1.2197483059051308 8 0.2467232074016962 0.8850781358041765 9 0.19429452582883577 0.7841239109390126 >10 0.607555898226677 4.609320979117688 >50 0.03392444101773323 1.069008435900982 >100 0.040092521202775636 4.40741252938736 >500 0.006168080185042405 1.938874291246024 >1k 0.006168080185042405 5.356105656202462 >5k 0.006168080185042405 18.95588438666851 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGT 7715 10.66934034020191 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 5992 8.286544046466602 No Hit TCTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCG 2444 3.3798921311021988 No Hit ATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGTCTT 1429 1.9762135251002628 TruSeq Adapter, Index 3 (95% over 23bp) TCTTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCC 747 1.0330521366339374 No Hit CTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGTC 655 0.9058221546120869 TruSeq Adapter, Index 3 (95% over 21bp) CTTTACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGTC 427 0.5905130687318491 RNA PCR Primer, Index 3 (95% over 21bp) ATTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGT 390 0.53934448900567 No Hit CATATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGT 306 0.4231779836813719 No Hit CTTATACACATCTCCGAGCCCACGAGACAACTAGGCATCTCGTATGCCGT 275 0.38030701147835705 No Hit TATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGTCT 258 0.35679712349605863 RNA PCR Primer, Index 3 (95% over 22bp) CTCTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCC 249 0.3443507122113124 No Hit CTTAATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCG 233 0.322223758816208 No Hit TCTTAATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCC 232 0.32084082422901394 No Hit CTTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCG 193 0.26690637532844697 No Hit TTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGTC 181 0.2503111602821187 TruSeq Adapter, Index 3 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGACGT 160 0.22126953395104412 No Hit CTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGA 154 0.21297192642788 No Hit ATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGTCAT 129 0.17839856174802932 Illumina PCR Primer Index 3 (95% over 22bp) CTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCACGTATGCCGT 95 0.13137878578343246 No Hit TCTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGT 91 0.12584704743465633 No Hit CTTATACACATCTCCGAGCCCACGAGACCACAAGGCATCTCGTATGCCGT 90 0.12446411284746232 No Hit TCTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGACG 84 0.11616650532429815 No Hit TCTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCAG 74 0.1023371594523579 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0013829345871940258 0.0 2 0.0 0.0 0.0 0.0013829345871940258 0.0 3 0.0 0.0 0.0 0.005531738348776103 0.0 4 0.0 0.0 0.0 0.012446411284746231 0.0 5 0.0 0.0 0.0 0.013829345871940258 0.0 6 0.0 0.0 0.0 0.015212280459134283 0.0 7 0.0 0.0 0.0 0.015212280459134283 0.0 8 0.0 0.0 0.0 0.015212280459134283 0.0 9 0.0 0.0 0.0 0.01936108422071636 0.0 10 0.0 0.0 0.0 0.01936108422071636 0.0 11 0.0 0.0 0.0 0.01936108422071636 0.0 12 0.0 0.0 0.0 0.024892822569492462 0.0 13 0.0 0.0 0.0 0.03319043009265662 0.0 14 0.0 0.0 0.0 0.03319043009265662 0.0 15 0.0 0.0 0.0 0.03319043009265662 0.0 16 0.0 0.0 0.0 0.03319043009265662 0.0 17 0.0 0.0 0.0 0.03319043009265662 0.0 18 0.0 0.0 0.0 0.03595629926704467 0.0 19 0.0 0.0 0.0 0.0428709722030148 0.0 20 0.0 0.0 0.0 0.05808325266214908 0.0 21 0.0 0.0 0.0 0.06361499101092519 0.0 22 0.0 0.0 0.0 0.06361499101092519 0.0 23 0.0 0.0 0.0 0.07191259853408934 0.0 24 0.0 0.0 0.0 0.08989074816761167 0.0 25 0.0 0.0 0.0 0.09403955192919375 0.0 26 0.0 0.0 0.0 0.0968054211035818 0.0 27 0.0 0.0 0.0 0.10925183238832803 0.0 28 0.0 0.0 0.0 0.12446411284746231 0.0 29 0.0 0.0 0.0 0.1341446549578205 0.0 30 0.0 0.0 0.0 0.17978149633522333 0.0 31 0.0 0.0 0.0 0.20052551514313374 0.0 32 0.0 0.0 0.0 0.22956714147420826 0.0 33 0.0 0.0 0.0 0.26552344074125295 0.0 34 0.0 0.0 0.0 0.32498962799059605 0.0 35 0.0 0.0 0.0 0.4245609182685659 0.0 36 0.0 0.0 0.0 0.5462591619416401 0.0 37 0.0 0.0 0.0 0.7910385838749827 0.0 38 0.0 0.0 0.0 1.0662425667265938 0.0 39 0.0 0.0 0.0 1.3774028488452497 0.0 40 0.0 0.0 0.0 1.718987691882174 0.0 41 0.0 0.0 0.0 2.151846217673904 0.0 42 0.0 0.0 0.0 2.646936799889365 0.0 43 0.0 0.0 0.0 3.190430092656617 0.0 44 0.0 0.0 0.0 3.6260544876227354 0.0 45 0.0 0.0 0.0 4.220716360116167 0.0 46 0.0 0.0 0.0 4.737933895726732 0.0 47 0.0 0.0 0.0 5.3007882727147 0.0 48 0.0 0.0 0.0 5.823537546674042 0.0 49 0.0 0.0 0.0 6.361499101092519 0.0 50 0.0 0.0 0.0 6.88424837505186 0.0 51 0.0 0.0 0.0 7.431890471580695 0.0 52 0.0 0.0 0.0 7.920066380860185 0.0 53 0.0 0.0 0.0 8.36813718711105 0.0 54 0.0 0.0 0.0 8.82173973171069 0.0 55 0.0 0.0 0.0 9.280874014659107 0.0 56 0.0 0.0 0.0 9.69437145623012 0.0 57 0.0 0.0 0.0 10.096805421103582 0.0 58 0.0 0.0 0.0 10.535195685244087 0.0 59 0.0 0.0 0.0 10.926566173419998 0.0 60 0.0 0.0 0.0 11.333148942055042 0.0 61 0.0 0.0 0.0 11.658138570045637 0.0 62 0.0 0.0 0.0 12.104826441709307 0.0 63 0.0 0.0 0.0 12.454708892269396 0.0 64 0.0 0.0 0.0 12.805974277416679 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGCATA 20 7.878553E-5 70.0 40 GCCCAGC 15 0.0022164583 70.0 6 CTCGTCC 15 0.0022164583 70.0 61 TTTACCG 20 7.878553E-5 70.0 26 AGCCCGG 15 0.0022164583 70.0 30 TTAGAGC 15 0.0022164583 70.0 32 GGACTAC 15 0.0022164583 70.0 10 AAGGTCG 15 0.0022164583 70.0 39 TCTCGTC 15 0.0022164583 70.0 60 CTTCTCG 15 0.0022164583 70.0 58 CGTAAGA 20 7.878553E-5 70.0 41 CGGCTCG 15 0.0022164583 70.0 14 CCGGTAA 20 7.878553E-5 70.0 33 AGCTCGG 15 0.0022164583 70.0 10 CGGTAAT 20 7.878553E-5 70.0 34 AAAGGGC 15 0.0022164583 70.0 69 ATGCCCT 15 0.0022164583 70.0 44 TTACCGT 20 7.878553E-5 70.0 27 CCCGGTA 20 7.878553E-5 70.0 32 ACGAGAG 15 0.0022164583 70.0 5 >>END_MODULE