FastQCFastQC Report
Wed 25 May 2016
SRR1780130_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780130_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113038
Sequences flagged as poor quality0
Sequence length76
%GC73

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5300546.8913108866045No Hit
CTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGTG39113.4598984412321525No Hit
TCTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGT3270.28928324988057114No Hit
ATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGTGGTC3270.28928324988057114No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGG2480.21939524761584603No Hit
TCTTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGG1420.12562147242520216No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTAGG207.8977675E-570.000011
GTGTTGC150.002219702370.06
TGTTGCC150.002219702370.07
TCCCCCC150.002219702370.09
TCGACGT150.002219702370.052
GTCGACG252.3817905E-455.99999651
TTGTGCG252.3817905E-455.9999962
GGTCGAC252.3817905E-455.99999650
TTTAAAG252.3817905E-455.99999653
TGACGAC252.3817905E-455.99999619
TGTGCGC252.3817905E-455.9999963
GGTAGCC252.3817905E-455.99999650
ATCTCGG6750.053.40740642
CTCGGTG6700.053.2835844
TCATTAA6650.053.15789460
GCCGTAT6550.052.9007654
CGGTGGT6750.052.8888946
TCGGTGG6750.052.8888945
TCGCCGT6400.052.50000452
TATGGAA200.006935906652.50000435
Produced by FastQC (version 0.11.5)