FastQCFastQC Report
Wed 25 May 2016
SRR1780130_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780130_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113038
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGT3072727.182894247952017No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG63735.637927068773333No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCG49264.3578265715953926No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCC13651.2075585201436685No Hit
ATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGTCTT13191.166864240343955TruSeq Adapter, Index 11 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCC10310.9120826624674888No Hit
CATATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGT7250.6413772359737433No Hit
CTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGTC6760.5980289814044835No Hit
ATTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGT5280.46709955944018827No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCCAACATCTCGTATGCCGT5130.4538296855924557Illumina PCR Primer Index 6 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGTC4100.36270988517135827No Hit
CTTTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCG3510.3105150480369433No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGACGT3250.2875139333675401No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCACACATCTCGTATGCCGT2770.24505033705479576No Hit
CTTAATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCG2520.22293388064190803No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTAAGCCGT2510.22204922238539251No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCC2320.20524071551159787No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCCCACATCACGTATGCCGT2200.19462481643341178No Hit
TTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGTC2150.19020152515083424No Hit
TATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGTCT2110.1866628921247722No Hit
CCTTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCG2020.17870096781613262No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGA1970.17427767653355508No Hit
ATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGTCAT1970.17427767653355508No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACCACATCTCGTATGCCGT1680.14862258709460535No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTAGTATGCCGT1240.10969762380792301No Hit
CTAATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGT1160.10262035775579893No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGAATGCCGT1150.10173569949928343No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCAAA207.8977675E-570.0000129
GCCCAAG207.8977675E-570.0000118
CATATCG401.3278623E-1070.0000136
ACACGCC353.6725396E-970.027
GTCTGGA150.002219702370.036
CGCATCT150.002219702370.034
ATCGTAA150.002219702370.039
AACGAGC150.002219702370.013
TGTGTTA150.002219702370.09
TCTTTAT1600.067.812511
CAACTCG650.064.6153936
AACTCGT550.063.6363637
ATATCGT453.8016879E-1062.22221837
TATCGTA453.8016879E-1062.22221838
AGGGGGG35450.062.1015670
GACACGC401.05555955E-861.25000426
TAAGCCG750.060.6666743
CATCACG1100.060.45454436
ACGCCCA352.9518378E-760.029
ATCGTAT352.9518378E-760.039
Produced by FastQC (version 0.11.5)