FastQCFastQC Report
Wed 25 May 2016
SRR1780129_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780129_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63575
Sequences flagged as poor quality0
Sequence length76
%GC87

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4485270.54974439638222No Hit
CTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGTG26394.151002752654345No Hit
TCTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGT2390.37593393629571376No Hit
ATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGTGGTC2050.3224537947306331No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGG1250.19661816751867872No Hit
TCTTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGG1100.1730239874164373No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGTA150.002215219270.036
ATCCAAA150.002215219270.015
CCAAATG150.002215219270.017
TTTCCGG150.002215219270.032
GTTCTTT150.002215219270.024
TTCCGAA150.002215219270.066
ACCACAC150.002215219270.053
ATGGTTC150.002215219270.021
TTTTCCG150.002215219270.031
TTTTTCC150.002215219270.030
GTATCCA150.002215219270.013
CAATATG150.002215219270.051
TCCGGAG150.002215219270.034
TATCCAA150.002215219270.014
CCACACC150.002215219270.054
GTAAGTT150.002215219270.043
AATATGG150.002215219270.052
CTATGTA150.002215219270.09
ACCTATG150.002215219270.07
ATTCCGA150.002215219270.065
Produced by FastQC (version 0.11.5)