FastQCFastQC Report
Wed 25 May 2016
SRR1780129_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780129_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63575
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGT2949446.392449862367286No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCG44346.974439638222572No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42136.626818718049547No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCC12651.9897758552890288No Hit
ATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGTCTT9861.5509241053873377RNA PCR Primer, Index 30 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCC8631.3574518285489579No Hit
CATATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGT6561.031852143138026No Hit
CTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGTC5540.8714117184427841No Hit
ATTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGT4390.6905230043255997No Hit
CTTTACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGTC3320.5222178529296106No Hit
CTTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCG2660.41840346047974836No Hit
CTTAATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCG2460.38694455367675973No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCC1950.3067243413291388No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGACGT1880.2957137239480928No Hit
TATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGTCT1870.29414077860794335No Hit
CCTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCG1780.2799842705465985No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATATCGTATGCCGT1660.2611089264648053No Hit
TTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGTC1610.2532441997640582No Hit
CTAATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGT1400.22021234762092018No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGA1260.19819111285882812No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCAGT1220.19189933149823044No Hit
CTTATACACATCTCCGAGCCCACGAGACGACTCTGGATCTCGTATGCCGT1220.19189933149823044No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCTCAGGATCTCGTATGCCGT1090.17145104207628786No Hit
CTTATACACATCTCCGAGCCCACGAGACGCATCTGGATCTCGTATGCCGT1030.16201337003539126No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTAAGCCGT950.14942980731419583No Hit
CTTATACACATCTCCGAGCCCACGAGCCGCCTCTGGATCTCGTATGCCGT910.14313802595359812No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCTCTGGCTCTCGTATGCCGT880.13841918993314983No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCACTGGATCTCGTATGCCGT800.12583562721195438No Hit
TCTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGT770.1211167911915061No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCAG700.11010617381046009No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGAATGCCGT680.10696028313016122No Hit
TCTTATACACATCTCCGCGCCCACGAGACGCCTCTGGATCTCGTATGCCG670.1053873377900118No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCTT252.8102095E-670.0000153
ACGCCAC252.8102095E-670.0000127
AGAAGCC252.8102095E-670.0000125
ATGACGT550.070.0000144
AAATACA252.8102095E-670.000012
TCTACTG252.8102095E-670.0000150
TATGACG500.070.0000143
GCCACTG252.8102095E-670.0000129
ACTGGAT252.8102095E-670.0000132
TGACGTC550.070.0000145
CGTCTAC353.6397978E-970.0000148
CAGCTAG150.002215219270.054
GAGAAGC301.0087933E-770.024
ACGCATC401.3096724E-1070.027
ACCACGA150.002215219270.019
AGCTAGA150.002215219270.055
GCAGGAA150.002215219270.031
GAAGCAG150.002215219270.035
CCGTCTA401.3096724E-1070.047
ACTCCGA207.8712146E-570.011
Produced by FastQC (version 0.11.5)