##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780129_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 63575 Sequences flagged as poor quality 0 Sequence length 76 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.081714510420763 32.0 32.0 32.0 27.0 32.0 2 29.827211954384584 32.0 32.0 32.0 27.0 32.0 3 30.340810066850178 32.0 32.0 32.0 27.0 32.0 4 30.952324026740072 32.0 32.0 32.0 27.0 32.0 5 30.828486040110107 32.0 32.0 32.0 27.0 32.0 6 33.408446716476604 37.0 32.0 37.0 22.0 37.0 7 35.38121903263862 37.0 37.0 37.0 32.0 37.0 8 33.67707432166732 37.0 32.0 37.0 27.0 37.0 9 35.43655524970507 37.0 37.0 37.0 32.0 37.0 10-11 34.93653165552497 37.0 37.0 37.0 29.5 37.0 12-13 35.341392056626034 37.0 37.0 37.0 32.0 37.0 14-15 35.250640975226105 37.0 37.0 37.0 32.0 37.0 16-17 33.13757766417617 37.0 32.0 37.0 24.5 37.0 18-19 34.04295713723948 37.0 37.0 37.0 24.5 37.0 20-21 35.23304758159654 37.0 37.0 37.0 32.0 37.0 22-23 34.344907589461265 37.0 34.5 37.0 27.0 37.0 24-25 34.18305937868659 37.0 34.5 37.0 27.0 37.0 26-27 34.18654345261502 37.0 34.5 37.0 27.0 37.0 28-29 34.82928824223359 37.0 37.0 37.0 29.5 37.0 30-31 34.87386551317341 37.0 37.0 37.0 29.5 37.0 32-33 35.030758946126625 37.0 37.0 37.0 29.5 37.0 34-35 34.16689736531656 37.0 37.0 37.0 24.5 37.0 36-37 33.81836413684624 37.0 34.5 37.0 27.0 37.0 38-39 32.88165945733385 37.0 32.0 37.0 22.0 37.0 40-41 33.920055053086905 37.0 34.5 37.0 24.5 37.0 42-43 34.22044828942194 37.0 37.0 37.0 27.0 37.0 44-45 34.46058198977586 37.0 37.0 37.0 27.0 37.0 46-47 33.97419583169484 37.0 34.5 37.0 27.0 37.0 48-49 32.72370428627605 37.0 32.0 37.0 17.5 37.0 50-51 33.49488792764451 37.0 34.5 37.0 22.0 37.0 52-53 32.867384978372 37.0 32.0 37.0 22.0 37.0 54-55 33.22158867479355 37.0 32.0 37.0 22.0 37.0 56-57 32.50185607550137 37.0 32.0 37.0 17.5 37.0 58-59 32.72533228470311 37.0 32.0 37.0 22.0 37.0 60-61 32.732064490758944 37.0 32.0 37.0 17.5 37.0 62-63 34.064333464412115 37.0 34.5 37.0 27.0 37.0 64-65 34.38581989775855 37.0 37.0 37.0 27.0 37.0 66-67 34.249909555642944 37.0 37.0 37.0 27.0 37.0 68-69 33.70257176563115 37.0 34.5 37.0 24.5 37.0 70-71 30.03432166732206 34.5 22.5 37.0 13.0 37.0 72-73 31.6621234762092 37.0 29.5 37.0 13.0 37.0 74-75 33.53119937082187 37.0 34.5 37.0 17.5 37.0 76 34.45725521038144 37.0 37.0 37.0 27.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 11101 1 -0.3924815027642623 11101 2 -3.0320801016577548 11101 3 0.6410799471385644 11101 4 -0.4620609538955378 11101 5 -0.6652674351279195 11101 6 1.3592047272398275 11101 7 -2.030664768953656 11101 8 0.8469402369451302 11101 9 -0.49102042928240763 11101 10-11 0.21998840360400607 11101 12-13 0.0030832494028629753 11101 14-15 -0.30077925082596124 11101 16-17 0.6665813611307811 11101 18-19 1.0911139185311143 11101 20-21 0.28130961018835166 11101 22-23 0.6490671762401377 11101 24-25 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0.06950376804196168 21612 16-17 1.1150314959016683 21612 18-19 1.1662486894206054 21612 20-21 0.8422934376816187 21612 22-23 1.359983618288659 21612 24-25 1.1109686993491152 21612 26-27 1.0060079416591066 21612 28-29 1.1793641183904953 21612 30-31 0.5557932093141247 21612 32-33 0.6492754202598263 21612 34-35 0.42661269715085126 21612 36-37 0.9598959559850613 21612 38-39 1.2873147696991722 21612 40-41 1.153367675413186 21612 42-43 1.560029310698262 21612 44-45 1.0122269183361325 21612 46-47 0.9602935479761214 21612 48-49 1.7962487079556055 21612 50-51 1.2008898764412095 21612 52-53 0.962528723303059 21612 54-55 1.8668362300134476 21612 56-57 0.4336064883230506 21612 58-59 0.6393118269944438 21612 60-61 2.156716601347611 21612 62-63 0.4576222527915448 21612 64-65 0.4722796813833767 21612 66-67 0.04758676362884984 21612 68-69 1.5244220067073755 21612 70-71 0.8868577241292428 21612 72-73 2.104513061646262 21612 74-75 1.3478944051247552 21612 76 0.7124232304480316 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 1.0 16 0.0 17 4.0 18 3.0 19 13.0 20 33.0 21 52.0 22 134.0 23 227.0 24 409.0 25 599.0 26 844.0 27 1140.0 28 1490.0 29 1978.0 30 2620.0 31 3513.0 32 5171.0 33 8005.0 34 12982.0 35 17040.0 36 7317.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 14.170887469720325 7.779910026111303 13.518104885645075 64.53109761852329 2 10.877088117784 7.5817157957655645 65.79261962437475 15.748576462075691 3 10.974439638222572 8.869838773102634 72.72984663782933 7.425874950845459 4 9.467558002359418 60.569406213134094 22.993314982304362 6.969720802202123 5 9.371608336610302 21.012976799056233 66.27447896185608 3.3409359024773893 6 11.602409828071664 68.0792160193793 13.732244820914538 6.586129331634499 7 11.482500983090837 19.63664962642548 9.101061738104601 59.77978765237908 8 11.279591034211562 67.49508454581203 5.404640188753441 15.820684231222964 9 11.243413291388125 15.663389697208022 3.8930397168698385 69.20015729453401 10-11 11.301003554912386 40.39623116368327 34.537704092868154 13.765061188536192 12-13 12.01494298073142 6.932756586708612 41.195438458513564 39.8568619740464 14-15 13.163979551710577 5.815965395202516 11.531262288635471 69.48879276445143 16-17 45.581596539520255 33.78136059771923 4.018875344081793 16.618167518678725 18-19 46.698387731026344 13.440817931576879 4.093590247738891 35.76720408965788 20-21 14.500983090837593 6.297286669288242 4.185607550137633 75.01612268973653 22-23 13.129581275364144 38.97583917953881 4.290275898952402 43.60430364614465 24-25 45.37425185022061 37.639897126947545 4.107843677003783 12.878007345828058 26-27 47.99314136496275 41.12034859484501 4.324400852610135 6.562109187582095 28-29 42.70579352220352 12.091205109247928 5.49307073980274 39.70993062874581 30-31 18.36972251934814 5.235795633297678 6.692411753602215 69.70207009375197 32-33 13.089095828352104 4.9793934436544385 38.708079028503114 43.22343169949034 34-35 41.15926071569013 6.192685804168305 40.905230043256 11.742823436885569 36-37 51.7294534014943 33.528902870625245 9.190719622493118 5.550924105387338 38-39 14.360990955564294 12.964215493511599 38.7432166732206 33.9315768777035 40-41 12.15984521974392 5.9253782993047475 41.41787523201309 40.496901248938244 42-43 45.00346053418064 6.008745713656526 37.238493723849366 11.749300028313462 44-45 14.460086511993708 37.860794337396776 41.100275265434526 6.57884388517499 46-47 44.75091626948547 7.519701760181209 13.38539946203577 34.34398250829755 48-49 42.257963035784506 5.0342115611482505 7.018482107746755 45.68934329532049 50-51 17.91034211561148 5.252064490758946 40.004718836020444 36.832874557609124 52-53 11.218246165945732 5.7097915847424305 70.07078254030672 13.001179709005111 54-55 11.302487632619997 5.773541278342732 44.38650716875477 38.537463920282505 56-57 44.072355485646874 5.1238694455367675 14.67400707825403 36.12976799056233 58-59 14.12122785078923 8.450715302519091 65.64267119678487 11.785385649906802 60-61 43.45812033031852 37.43138025953598 14.566260322453795 4.544239087691702 62-63 12.6220998820291 78.00865119937083 4.763664962642548 4.60558395595753 64-65 10.170118052333125 80.94017161238565 4.231322799594171 4.658387535687039 66-67 10.260483845597257 80.8034731179413 4.037814200773901 4.898228835687545 68-69 12.527723161620132 78.72827369248918 4.065277231616201 4.678725914274479 70-71 39.54069996067637 51.09634290208416 4.116397955171058 5.246559182068423 72-73 83.43741152043287 7.083084279736999 4.068487117375027 5.411017082455092 74-75 86.68952716519331 4.243086796489131 4.053543901594992 5.013842136722559 76 86.90796407279821 3.916757113869095 4.294275871832381 4.881002941500323 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 1.0 15 0.5 16 4.0 17 8.0 18 4.5 19 5.0 20 12.0 21 15.0 22 19.5 23 17.5 24 15.0 25 19.0 26 27.5 27 33.0 28 44.0 29 58.0 30 72.0 31 134.5 32 189.0 33 195.0 34 195.0 35 235.0 36 289.0 37 303.0 38 316.5 39 401.0 40 524.5 41 602.0 42 627.0 43 673.5 44 795.0 45 942.0 46 1014.0 47 1231.0 48 2483.5 49 7751.5 50 11984.0 51 20966.5 52 16122.5 53 1939.0 54 1582.0 55 1012.0 56 363.0 57 264.0 58 244.0 59 226.0 60 176.5 61 141.0 62 137.0 63 126.5 64 121.0 65 117.0 66 91.0 67 74.0 68 69.0 69 65.0 70 60.0 71 54.0 72 47.5 73 46.0 74 45.0 75 39.0 76 31.5 77 29.0 78 26.5 79 19.0 80 17.5 81 15.5 82 17.5 83 20.0 84 14.5 85 7.5 86 7.5 87 9.0 88 7.0 89 7.0 90 8.5 91 6.5 92 5.0 93 6.0 94 9.5 95 14.0 96 16.0 97 28.5 98 91.0 99 2141.5 100 4142.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.00157294534014943 2 0.00157294534014943 3 0.0 4 0.0 5 0.0 6 0.00314589068029886 7 0.0 8 0.0 9 0.0 10-11 0.00157294534014943 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.00157294534014943 24-25 0.0023594180102241447 26-27 0.008651199370821865 28-29 0.00629178136059772 30-31 0.0047188360204482895 32-33 0.0047188360204482895 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.00157294534014943 42-43 0.00157294534014943 44-45 0.0 46-47 0.00314589068029886 48-49 0.0 50-51 0.0 52-53 0.0 54-55 7.86472670074715E-4 56-57 0.0 58-59 7.86472670074715E-4 60-61 0.0 62-63 0.0 64-65 0.0023594180102241447 66-67 0.00157294534014943 68-69 0.0 70-71 0.0 72-73 0.00157294534014943 74-75 0.00157294534014943 76 0.00314589068029886 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 63575.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 14.515139598898937 #Duplication Level Percentage of deduplicated Percentage of total 1 70.1127004768097 10.176956350766812 2 12.201993931512787 3.5422729060165157 3 6.827048114434331 2.9728666928824223 4 4.107065452969224 2.3845851356665353 5 2.4057217165149547 1.7459693275658672 6 1.1270047680970958 0.9815178922532442 7 0.8885999133073256 0.9028706252457728 8 0.41179020372778496 0.47817538340542665 9 0.30342436064152584 0.39638222571765624 >10 1.2353706111833551 3.488792764451435 >50 0.11920242739488512 1.2898151789225325 >100 0.17338534893801474 4.844671647660244 >500 0.043346337234503686 4.811639795517106 >1k 0.032509752925877766 15.591034211561148 >5k 0.0 0.0 >10k+ 0.010836584308625921 46.392449862367286 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGT 29494 46.392449862367286 No Hit TCTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCG 4434 6.974439638222572 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 4213 6.626818718049547 No Hit CTCTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCC 1265 1.9897758552890288 No Hit ATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGTCTT 986 1.5509241053873377 RNA PCR Primer, Index 30 (95% over 21bp) TCTTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCC 863 1.3574518285489579 No Hit CATATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGT 656 1.031852143138026 No Hit CTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGTC 554 0.8714117184427841 No Hit ATTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGT 439 0.6905230043255997 No Hit CTTTACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGTC 332 0.5222178529296106 No Hit CTTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCG 266 0.41840346047974836 No Hit CTTAATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCG 246 0.38694455367675973 No Hit TCTTAATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCC 195 0.3067243413291388 No Hit CTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGACGT 188 0.2957137239480928 No Hit TATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGTCT 187 0.29414077860794335 No Hit CCTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCG 178 0.2799842705465985 No Hit CTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATATCGTATGCCGT 166 0.2611089264648053 No Hit TTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGTC 161 0.2532441997640582 No Hit CTAATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGT 140 0.22021234762092018 No Hit CTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGA 126 0.19819111285882812 No Hit CTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCAGT 122 0.19189933149823044 No Hit CTTATACACATCTCCGAGCCCACGAGACGACTCTGGATCTCGTATGCCGT 122 0.19189933149823044 No Hit CTTATACACATCTCCGAGCCCACGAGACGCCTCAGGATCTCGTATGCCGT 109 0.17145104207628786 No Hit CTTATACACATCTCCGAGCCCACGAGACGCATCTGGATCTCGTATGCCGT 103 0.16201337003539126 No Hit CTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTAAGCCGT 95 0.14942980731419583 No Hit CTTATACACATCTCCGAGCCCACGAGCCGCCTCTGGATCTCGTATGCCGT 91 0.14313802595359812 No Hit CTTATACACATCTCCGAGCCCACGAGACGCCTCTGGCTCTCGTATGCCGT 88 0.13841918993314983 No Hit CTTATACACATCTCCGAGCCCACGAGACGCCACTGGATCTCGTATGCCGT 80 0.12583562721195438 No Hit TCTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGT 77 0.1211167911915061 No Hit TCTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCAG 70 0.11010617381046009 No Hit CTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGAATGCCGT 68 0.10696028313016122 No Hit TCTTATACACATCTCCGCGCCCACGAGACGCCTCTGGATCTCGTATGCCG 67 0.1053873377900118 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.006291781360597719 0.0 6 0.0 0.0 0.0 0.006291781360597719 0.0 7 0.0 0.0 0.0 0.006291781360597719 0.0 8 0.0 0.0 0.0 0.009437672040896579 0.0 9 0.0 0.0 0.0 0.012583562721195438 0.0 10 0.0 0.0 0.0 0.012583562721195438 0.0 11 0.0 0.0 0.0 0.015729453401494297 0.0 12 0.0 0.0 0.0 0.015729453401494297 0.0 13 0.0 0.0 0.0 0.015729453401494297 0.0 14 0.0 0.0 0.0 0.015729453401494297 0.0 15 0.0 0.0 0.0 0.015729453401494297 0.0 16 0.0 0.0 0.0 0.01730239874164373 0.0 17 0.0 0.0 0.0 0.01730239874164373 0.0 18 0.0 0.0 0.0 0.023594180102241447 0.0 19 0.0 0.0 0.0 0.0424695241840346 0.0 20 0.0 0.0 0.0 0.05662603224537947 0.0 21 0.0 0.0 0.0 0.06763664962642549 0.0 22 0.0 0.0 0.0 0.07235548564687377 0.0 23 0.0 0.0 0.0 0.08179315768777035 0.0 24 0.0 0.0 0.0 0.08651199370821865 0.0 25 0.0 0.0 0.0 0.0896578843885175 0.0 26 0.0 0.0 0.0 0.0896578843885175 0.0 27 0.0 0.0 0.0 0.09594966574911522 0.0 28 0.0 0.0 0.0 0.10696028313016123 0.0 29 0.0 0.0 0.0 0.12268973653165552 0.0 30 0.0 0.0 0.0 0.14313802595359812 0.0 31 0.0 0.0 0.0 0.14942980731419583 0.0 32 0.0 0.0 0.0 0.16201337003539126 0.0 33 0.0 0.0 0.0 0.18088871411718443 0.0 34 0.0 0.0 0.0 0.2107746755800236 0.0 35 0.0 0.0 0.0 0.24537947306331104 0.0 36 0.0 0.0 0.0 0.3098702320094377 0.0 37 0.0 0.0 0.0 0.46087298466378296 0.0 38 0.0 0.0 0.0 0.6087298466378294 0.0 39 0.0 0.0 0.0 0.7141171844278411 0.0 40 0.0 0.0 0.0 0.8997247345654739 0.0 41 0.0 0.0 0.0 1.0475815965395203 0.0 42 0.0 0.0 0.0 1.197011403853716 0.0 43 0.0 0.0 0.0 1.3590247738891073 0.0 44 0.0 0.0 0.0 1.4958710184821078 0.0 45 0.0 0.0 0.0 1.7365316555249706 0.0 46 0.0 0.0 0.0 1.9551710578057413 0.0 47 0.0 0.0 0.0 2.1643727880456156 0.0 48 0.0 0.0 0.0 2.348407392843099 0.0 49 0.0 0.0 0.0 2.4915454187966968 0.0 50 0.0 0.0 0.0 2.637829335430594 0.0 51 0.0 0.0 0.0 2.81399921352733 0.0 52 0.0 0.0 0.0 2.985450255603618 0.0 53 0.0 0.0 0.0 3.177349587101848 0.0 54 0.0 0.0 0.0 3.3189146677152968 0.0 55 0.0 0.0 0.0 3.4589068029885963 0.0 56 0.0 0.0 0.0 3.597325992921746 0.0 57 0.0 0.0 0.0 3.7121510027526545 0.0 58 0.0 0.0 0.0 3.8804561541486433 0.0 59 0.0 0.0 0.0 4.009437672040897 0.0 60 0.0 0.0 0.0 4.1447109712937475 0.0 61 0.0 0.0 0.0 4.246952418403461 0.0 62 0.0 0.0 0.0 4.3853716083366105 0.0 63 0.0 0.0 0.0 4.5237907982697605 0.0 64 0.0 0.0 0.0 4.659064097522611 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTGCTT 25 2.8102095E-6 70.00001 53 ACGCCAC 25 2.8102095E-6 70.00001 27 AGAAGCC 25 2.8102095E-6 70.00001 25 ATGACGT 55 0.0 70.00001 44 AAATACA 25 2.8102095E-6 70.00001 2 TCTACTG 25 2.8102095E-6 70.00001 50 TATGACG 50 0.0 70.00001 43 GCCACTG 25 2.8102095E-6 70.00001 29 ACTGGAT 25 2.8102095E-6 70.00001 32 TGACGTC 55 0.0 70.00001 45 CGTCTAC 35 3.6397978E-9 70.00001 48 CAGCTAG 15 0.0022152192 70.0 54 GAGAAGC 30 1.0087933E-7 70.0 24 ACGCATC 40 1.3096724E-10 70.0 27 ACCACGA 15 0.0022152192 70.0 19 AGCTAGA 15 0.0022152192 70.0 55 GCAGGAA 15 0.0022152192 70.0 31 GAAGCAG 15 0.0022152192 70.0 35 CCGTCTA 40 1.3096724E-10 70.0 47 ACTCCGA 20 7.8712146E-5 70.0 11 >>END_MODULE