FastQCFastQC Report
Wed 25 May 2016
SRR1780128_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780128_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19000
Sequences flagged as poor quality0
Sequence length76
%GC70

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG778340.96315789473684No Hit
CTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGTG259913.678947368421051No Hit
ATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGTGGTC2841.4947368421052631No Hit
TCTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGT2201.1578947368421053No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGG1600.8421052631578947No Hit
TCTTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGG890.46842105263157896No Hit
CTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGTGG490.2578947368421053No Hit
CTTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGT370.19473684210526315No Hit
CTTAATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGT360.1894736842105263No Hit
CTTTACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGTGG350.18421052631578946No Hit
CTTATACACATCTGACGCTGCCGACGAGGCAACCAGTGTAGATCTCGGTG350.18421052631578946No Hit
TCTTAATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGG350.18421052631578946No Hit
TATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGTGGT350.18421052631578946No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG320.16842105263157894No Hit
CTTATACACATCTGACGCTGCCTGTCTCTTATACACCTGTCTCTTATACA240.12631578947368421No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTGA150.002191288470.015
GTATCAA150.002191288470.057
AAAAAGG2250.059.1111170
GTGGTCG4350.057.93103848
CCGTATC4050.057.90123455
CGCCGTA4050.057.90123453
GGTGGTC4300.057.790747
TGGTCGC4250.057.64705749
CGTATCA4200.057.49999656
TCTCGGT4150.057.34939243
TCGCCGT4150.057.34939252
CTCGGTG4150.057.34939244
GCCGTAT4100.057.1951254
TCGGTGG4350.057.1264445
CGGTGGT4350.057.1264446
ATCTCGG4250.056.8235342
GTCGCCG4250.056.8235351
CATTAAA4250.056.8235361
GATCTCG4250.056.8235341
TTAAAAA4250.056.8235363
Produced by FastQC (version 0.11.5)