FastQCFastQC Report
Wed 25 May 2016
SRR1780128_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780128_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19000
Sequences flagged as poor quality0
Sequence length76
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG444023.36842105263158No Hit
ATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGTCTT13457.078947368421053TruSeq Adapter, Index 6 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGTC4872.5631578947368423No Hit
CTTTACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGTC4142.1789473684210527No Hit
CTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGT4002.1052631578947367No Hit
TCTTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCC3511.8473684210526315No Hit
CTTAATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCG2631.3842105263157896No Hit
TCTTAATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCC2201.1578947368421053No Hit
ATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGTCAT1991.0473684210526315No Hit
TATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGTCT1800.9473684210526316No Hit
TCTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCG1670.8789473684210526No Hit
TTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGTC1510.7947368421052632No Hit
TCTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGT890.46842105263157896No Hit
CTTATATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCC490.2578947368421053No Hit
ATTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGT460.24210526315789474No Hit
TCTTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGAC440.23157894736842108No Hit
CTTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCG410.21578947368421053No Hit
CTTATACACATCTCCGAGCCCACGAGACACAACAACATCTCGTATGCCGT390.20526315789473684No Hit
ATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGACTT330.1736842105263158No Hit
CTTATTACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCG320.16842105263157894No Hit
TCTTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCA300.15789473684210525No Hit
CTTACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGTCT290.15263157894736842No Hit
CATATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGT280.1473684210526316No Hit
ACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGTCTTCT270.14210526315789473RNA PCR Primer, Index 6 (95% over 23bp)
GTGTATAAGAGACAGGCAGCGTCAGATGTGTATAAGCTGTCTCTTATACA260.13684210526315788No Hit
ATACACATCTCCGCGCCCACGAGACACCACAACATCTCGTATGCCGTCTT240.12631578947368421TruSeq Adapter, Index 6 (95% over 21bp)
TATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGTCA230.12105263157894737No Hit
ATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGACGTCTT230.12105263157894737No Hit
CTTTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCC220.11578947368421054No Hit
CTTAACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGTC210.11052631578947368No Hit
TCTCATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCG200.10526315789473684No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTTAT550.070.000011
TTTACAC353.4578989E-970.000012
ACTCGTA207.729976E-570.038
AACTCGT150.002191288470.037
AATATAC252.7348742E-670.01
AATGCCG150.002191288470.042
TCTTAAT207.729976E-570.01
CTTTATA650.064.615392
TTTATAC650.064.615393
CTTTACA409.940777E-961.251
TTACACA409.940777E-961.253
GTATGAC308.068584E-658.33333242
CACGTAT252.3314875E-455.99999639
TATGACG252.3314875E-455.99999643
GAGACGC252.3314875E-455.99999624
CTATACA1250.053.1999971
ATGACGA200.006847798452.544
ACCACGA200.006847798452.519
TAAGCAT200.006847798452.553
CAACTCG200.006847798452.536
Produced by FastQC (version 0.11.5)