FastQCFastQC Report
Wed 25 May 2016
SRR1780127_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780127_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106698
Sequences flagged as poor quality0
Sequence length76
%GC88

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7759172.72020094097358No Hit
CTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGTG88118.257886745768431No Hit
TCTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGT8120.7610264484807587No Hit
ATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGTGGTC6090.570769836360569No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGG5110.47892181671633954No Hit
TCTTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGG3050.2858535305254082No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2140.20056608371290932No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1640.15370484920054733No Hit
CTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGTGG1370.12839978256387186No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1190.11152973813942153No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGTCG150.002220184369.9934448
CGGAGGT150.002220184369.9934446
AGGGATG150.002220184369.9934424
ACATTAA150.002220184369.9934460
AGGTGGT150.002220184369.9934446
CCGTAAC207.899401E-569.9934455
ACGAGGA207.899401E-569.9934424
GGGGGAA150.002220184369.9934461
CTTGTGC150.002220184369.993441
GGAGGTC150.002220184369.9934447
TGGCGCT401.3278623E-1069.9934413
GCCGTAA207.899401E-569.9934454
TTGTGCG207.899401E-569.993442
TCTTTAT301.0160511E-769.993441
TGTGCGG150.002220184369.993443
TGTGCGC150.002220184369.993443
TAGATAA150.002220184369.9934439
TTAAACA207.899401E-569.993442
ATTTAAA150.002220184369.9934462
TAAACAC252.8249215E-669.993433
Produced by FastQC (version 0.11.5)