FastQCFastQC Report
Wed 25 May 2016
SRR1780127_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780127_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106698
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGT4745344.474123226302275No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1349212.645035520815762No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCG71626.712403231550732No Hit
ATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGTCTT22882.1443700912856847Illumina PCR Primer Index 10 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCC21922.0543965210219497No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCC15541.4564471686442106No Hit
CATATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGT11761.102176235730754No Hit
CTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGTC10711.003767643254794No Hit
ATTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGT9200.8622467150274606No Hit
CTTTACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGTC6620.6204427449436728No Hit
CTTATACACATCTCCGAGCCCACGAGACTTAAACTAATCTCGTATGCCGT5900.5529625672458716No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCG5820.5454647697238936No Hit
CTTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGACGT4260.3992577180453242No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCG4090.3833248983111211No Hit
TTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGTC3880.3636431798159291No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCC3690.3458359107012315No Hit
TATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGTCT3470.3252169675157922Illumina PCR Primer Index 10 (95% over 21bp)
CCTTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCG3050.2858535305254082No Hit
CTTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGA3030.28397908114491366No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAAACTTATCTCGTATGCCGT2890.2708579354814523No Hit
CTTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCAGT2300.21556167875686516No Hit
CTTATACACATCTCCGAGCCCACGAGACTTAAAATTATCTCGTATGCCGT2280.21368722937637072No Hit
CTAATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGT2100.19681718495192035No Hit
CTTATACACATCTCCGAGCCCACGAGACTTAAACTTAACTCGTATGCCGT1920.17994714052747005No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAACTTATCTCGTATGCCGT1820.17057489362499767No Hit
CTTATACACATCTCCGAGCCCACGAGACTTAAACATATCTCGTATGCCGT1760.16495154548351423No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCAG1710.16026542203227803No Hit
TCTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGT1470.13777202946634426No Hit
CTTATACACATCTCCGAGCCCACGAGACTTAAACTTATATCGTATGCCGT1440.13496035539560253No Hit
CTTATACACATCACCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGT1330.12465088380288289No Hit
CTTATACACATCTCCGAGCCAACGAGACTTAAACTTATCTCGTATGCCGT1180.11059251344917431No Hit
CTTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGAATGCCGT1140.10684361468818536No Hit
AATATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGT1120.10496916530769088No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAAACTAATCTCGTATGCCGT1110.10403194061744361No Hit
CTTATACACATCTCCGAGACCACGAGACTTAAACTTATCTCGTATGCCGT1110.10403194061744361No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGACA150.002213521970.04645521
ACGACAC150.002213521970.04645522
CTACAGC207.888124E-570.013651
TCTACAG207.888124E-570.013650
TTCTCCT207.888124E-570.013652
TACTGCA150.002217647270.013652
GTCTACA207.888124E-570.013649
CGACTAC150.002217647270.013648
GATAGAA150.002217647270.013656
GTATGAA207.906482E-569.9807842
AACGTAT207.906482E-569.9807839
CTTTACA900.069.980781
TCTATAC207.906482E-569.980782
TCGTATC207.906482E-569.9807840
AATATAC353.6779966E-969.980781
TGCACAT150.00222177869.9807745
TCAAGTA150.00222177869.98077438
ATGACGA252.8279574E-669.98077444
TAACGTA150.00222177869.98077438
TATGCCT150.00222177869.98077443
Produced by FastQC (version 0.11.5)