FastQCFastQC Report
Wed 25 May 2016
SRR1780126_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780126_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155593
Sequences flagged as poor quality0
Sequence length76
%GC81

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9016357.94797966489496No Hit
CTTATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGGTG67274.323459281587218No Hit
TCTTATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGGT5360.34448850526694647No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4520.2905015007101862No Hit
CTCTTATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGG3940.2532247594686136No Hit
ATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGGTGGTC3810.24486962781101979No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2500.16067560879988174No Hit
TCTTTATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGG1740.11183022372471771No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACCT150.002217920370.02669528
GCGACAC150.002217920370.02669531
ACCTCTT150.002217920370.02669531
CCTTTAT150.002217920370.02669532
CGATGAT150.002217920370.02669527
TCCGGCG207.904771E-570.0041824
GAACTCG207.904771E-570.0041841
ATAGCTG150.002220752270.0041837
CACGCAG150.002220752270.0041839
TTATAGC150.002220752270.0041835
ATCCGGC150.002220752270.0041823
ACTCGGT150.002220752270.0041843
AACTCGG207.904771E-570.0041842
ACTTATA150.002223586769.981681
TGTGCGG150.002223586769.981683
GCTCCAT150.002223586769.9816814
ACGACAA150.002223586769.9816819
ACTGGCC150.002223586769.981688
GGGCGCT750.060.65078713
TTGTGCG352.964498E-759.9842952
Produced by FastQC (version 0.11.5)