FastQCFastQC Report
Wed 25 May 2016
SRR1780126_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780126_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155593
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGT5300534.06644257775093No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG107376.9006960467373215No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCG71014.563829992351841No Hit
ATTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGT55433.562499598310978No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCC27271.7526495407891098No Hit
CTTATACACATCTCCGAGCCCACGAGACAATAAGACATCACGTATGCCGT14170.9107093506777297No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCC14120.9074958385017321No Hit
CTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTAGTATGCCGT12660.8136612829626012No Hit
ATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGTCTT9140.5874300257723677RNA PCR Primer, Index 41 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTAAGCCGT7770.4993797921500324No Hit
CTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGACGT7480.4807414215292462No Hit
CTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGTC6430.4132576658332958No Hit
CTTATACACATCTCCGAGCCCACGAGACAATAAGCCATCTCGTATGCCGT4630.297571227497381No Hit
CTTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCG3950.25386746190381315No Hit
ATTATACACATCTCCGAGCCCACGAGACAATAAGACATCTAGTATGCCGT3950.25386746190381315No Hit
ATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGTCAT3550.22815936449583207No Hit
CTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGA3360.21594801822704107No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTAGTATGCCG3180.2043793743934496No Hit
CTTATACACATCTCCGAGCCCACGAGACAAAAAGACATCTCGTATGCCGT3180.2043793743934496No Hit
CTTTACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGTC3100.19923775491185336No Hit
ATTATACACATCTCCGAGCCCACGAGACAATAAGACATCACGTATGCCGT2990.19216802812465858No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCC2850.18317019403186519No Hit
ATTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGACGT2840.18252749159666568No Hit
ATCTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCC2740.17610046724467038No Hit
CTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCCT2710.17417235993907182No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATAAGACATCACGTATGCCG2610.16774533558707652No Hit
CTTAATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCG2540.16324641854067987No Hit
CTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGAATGCCGT2280.14653615522549215No Hit
CCTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCG2160.1388237260030978No Hit
ATTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTAAGCCGT2100.13496751139190066No Hit
TTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGTC2080.13368210652150161No Hit
CTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATTCCGT2030.13046859434550395No Hit
CTTATACACATCTCCGAGCCCACGAGACAATAAGACATCAAGTATGCCGT1950.12532697486390776No Hit
TATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGTCT1940.12468427242870823RNA PCR Primer, Index 41 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAATAAGAAATCTCGTATGCCGT1700.10925941398391958No Hit
CTTATACACATCTCCGAGCCCACGAGACAATAAGACATATCGTATGCCGT1600.10283238963192433No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCTT150.002216628370.0369926
TTATCTT150.002219458670.0144732
ATCCATA150.002219458670.0144720
TACATCT207.899021E-570.01446534
CGAAATA207.911616E-569.9919748
ATCACGA207.911616E-569.9919737
GCCGAAA207.911616E-569.9919746
TCGTAAT401.3460522E-1069.9919740
CGCGTAT207.911616E-569.9919739
CATCATG207.911616E-569.9919754
GCGTATG207.911616E-569.9919740
TCGCGTA207.911616E-569.9919738
ACACGAA150.002222291469.9919638
GATGTTC150.002222291469.9919612
TAGGTAA150.002222291469.991968
GATCCCA150.002222291469.9919616
GTCTAAT150.002222291469.9919649
CACGAAT301.0192889E-769.9919639
CGACAAC150.002222291469.9919648
ATGACGA301.0192889E-769.9919644
Produced by FastQC (version 0.11.5)