FastQCFastQC Report
Wed 25 May 2016
SRR1780125_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780125_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86461
Sequences flagged as poor quality0
Sequence length76
%GC92

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6812678.79390707949248No Hit
CTTATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGGTG47025.4382900961126985No Hit
TCTTATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGGT4070.4707324689744509No Hit
ATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGGTGGTC2780.32153225153537435No Hit
CTCTTATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGG2400.2775817998866541No Hit
TCTTTATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGG1540.17811498826060304No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1390.16076612576768717No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1150.13300794577902175No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG880.10177999329177317No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGG150.002217932170.05
AACGAAG150.002217932170.030
ACACATA150.002217932170.06
TCTCGGG150.002217932170.043
TCGCCGA150.002217932170.052
GTGCGCG150.002217932170.04
GTTATAC150.002217932170.01
CCGAATC150.002217932170.055
CGGGCGC352.9436524E-760.012
CGACGAA308.314995E-658.33333222
AAAAAGG4600.057.82608470
GTGTAGG252.3786468E-455.99999636
GACGAAG252.3786468E-455.99999623
AGATCTC7550.054.2384140
TCGCCGT7250.054.06896652
GCCGTAT7250.054.06896654
GTAGATC7450.054.02684438
TAGATCT7450.054.02684439
CCGTATC7200.053.95833655
CGTATCA7200.053.95833656
Produced by FastQC (version 0.11.5)