FastQCFastQC Report
Wed 25 May 2016
SRR1780125_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780125_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86461
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGT4201348.59185066099166No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG73218.467401487375811No Hit
TCTTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCG61717.137322029585594No Hit
ATTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGT59896.926822498004881No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCC19372.2403164432518707No Hit
ATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGTCTT11371.3150437769630237Illumina PCR Primer Index 5 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCC10671.2340824186627497No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCATTGCTCTCGTATGCCGT7220.8350585813256844No Hit
CTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGTC5850.6766056372237194No Hit
CTTATACACATCTCCGAGCCCACGAGACAACCATTGATCTCGTATGCCGT4500.5204658747874765No Hit
CTTTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCG3180.3677958848498167No Hit
CTTATACACATCTCCGAGCCCACGAGACCACAATTGATCTCGTATGCCGT2930.33888111402829024No Hit
CTTTACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGTC2740.31690588820393006No Hit
TCTTATACACATCTCCGAGCCCACGAGACCACCATTGCTCTCGTATGCCG2660.3076531615410416No Hit
ATCTTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCC2380.275268618220932No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCC2360.2729554365552099No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCATTGATATCGTATGCCGT2210.255606574062294No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCAATGATCTCGTATGCCGT2110.24404066573368338No Hit
TTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGTC2040.23594452990365597No Hit
CTTAATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCG1930.22322203074218433No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCATAGATCTCGTATGCCGT1930.22322203074218433No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGACGT1870.21628248574501796No Hit
TATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGTCT1730.20009021408496316Illumina PCR Primer Index 5 (95% over 21bp)
ATTATACACATCTCCGAGCCCACGAGACCACCATTGCTCTCGTATGCCGT1650.1908374874220747No Hit
CCTTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCG1560.18042816992632518No Hit
ATTATACACATCTCCGAGCCCACGAGACCACCATTGATATCGTATGCCGT1530.176958397427742No Hit
CTTATACACATCTCCGAGCCCACGAGACCAACATTGATCTCGTATGCCGT1520.17580180659488093No Hit
CTTATACACATCTCCGAGCCCACGAGACCCCCATTGATCTCGTATGCCGT1450.16770567076485351No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCAGT1330.1538265807705208No Hit
CTTATACACATCTCCGAGCCCACGAGCCCACCATTGATCTCGTATGCCGT1300.15035680827193762No Hit
CTAATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGT1260.1457304449404934No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCACCATTGCTCTCGTATGCC1090.12606840078185538No Hit
ATTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGACGT1040.12028544661755011No Hit
ATTATACACATCTCCGAGCCCACGAGACAACCATTGATCTCGTATGCCGT1030.11912885578468904No Hit
TCTTATACACATCTCCGAGCCCACGAGCCCACCATTGATCTCGTATGCCG990.11450249245324481No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGA950.10987612912180059No Hit
CTTATACACATCTCCGAGACCACGAGACCACCATTGATCTCGTATGCCGT900.10409317495749529No Hit
TCTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGT900.10409317495749529No Hit
ATTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCAGT880.10177999329177317No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAGA650.070.0000113
ATGGGGG252.818817E-670.070
CAAGAAA150.002217932170.057
AATCTCC150.002217932170.09
AAATCTC150.002217932170.08
GCAAGAA150.002217932170.056
ATGACGA150.002217932170.044
TATGCTT301.0131225E-770.053
ACCACGA600.070.019
CAACTCA150.002217932170.09
CATCTGC600.070.051
TAAGACG150.002217932170.043
TTATAAA150.002217932170.02
TATTATA252.818817E-670.01
TCTTTAT1200.070.01
GACCACG600.070.018
CGTCTTA301.0131225E-770.048
CGTCTAC353.6598067E-970.048
TGACGAC150.002217932170.045
TCTTAAT150.002217932170.01
Produced by FastQC (version 0.11.5)