FastQCFastQC Report
Wed 25 May 2016
SRR1780124_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780124_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142884
Sequences flagged as poor quality0
Sequence length76
%GC81

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8342758.387923070462755No Hit
CTTATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGGTG115648.093278463648835No Hit
TCTTATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGGT8590.6011869768483525No Hit
ATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGGTGGTC7120.4983063184121385No Hit
CTCTTATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGG4360.3051426331849612No Hit
TCTTTATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGG3370.2358556591360824No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3020.2113602642703172No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1680.11757789535567313No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTGC301.0178701E-770.01
CGGGTGT207.90491E-570.07
CGTGGGC150.002220907270.01
TTGTGCG301.0178701E-770.02
GCTGCGC150.002220907270.08
CCATGCA150.002220907270.09
TGCGCTA150.002220907270.010
GTAGCAC150.002220907270.055
CGTGTGC150.002220907270.01
TCTTTAT500.070.01
TAAGAAC150.002220907270.021
GTTATAC150.002220907270.01
CCATTGA150.002220907270.048
TGTGCGC401.058288E-861.253
CGCTGGC352.957422E-760.016
TGCGGGC308.34274E-658.33333219
CGTCTGG553.45608E-1157.2727249
CTTATGC252.3839314E-455.9999961
CGGTGGA252.3839314E-455.99999647
AAAAAGG10400.055.52884770
Produced by FastQC (version 0.11.5)