##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780124_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 142884 Sequences flagged as poor quality 0 Sequence length 76 %GC 81 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.43060804568741 32.0 27.0 32.0 13.0 32.0 2 29.277441840933903 32.0 32.0 32.0 13.0 32.0 3 29.121728115114358 32.0 32.0 32.0 13.0 32.0 4 28.469779681419894 32.0 27.0 32.0 13.0 32.0 5 29.354294392654182 32.0 32.0 32.0 22.0 32.0 6 33.423294420648915 37.0 37.0 37.0 22.0 37.0 7 33.0388636936256 37.0 37.0 37.0 13.0 37.0 8 33.36363063744016 37.0 37.0 37.0 13.0 37.0 9 33.47729626830156 37.0 37.0 37.0 22.0 37.0 10-11 33.34762114722432 37.0 37.0 37.0 17.5 37.0 12-13 33.07872819909857 37.0 37.0 37.0 13.0 37.0 14-15 33.53173903306178 37.0 34.5 37.0 20.0 37.0 16-17 33.93227373253828 37.0 37.0 37.0 24.5 37.0 18-19 33.49081422692534 37.0 37.0 37.0 22.0 37.0 20-21 33.90459043699784 37.0 37.0 37.0 24.5 37.0 22-23 33.99902718288962 37.0 37.0 37.0 24.5 37.0 24-25 33.45001889644747 37.0 37.0 37.0 17.5 37.0 26-27 33.85058509000308 37.0 37.0 37.0 24.5 37.0 28-29 33.72611349066376 37.0 37.0 37.0 22.0 37.0 30-31 33.7494680999972 37.0 37.0 37.0 22.0 37.0 32-33 33.83435864057557 37.0 37.0 37.0 22.0 37.0 34-35 33.81093054505753 37.0 37.0 37.0 22.0 37.0 36-37 34.48689846308894 37.0 37.0 37.0 29.5 37.0 38-39 34.617301447327904 37.0 37.0 37.0 29.5 37.0 40-41 34.11555877495031 37.0 37.0 37.0 24.5 37.0 42-43 33.970297584054194 37.0 37.0 37.0 22.0 37.0 44-45 33.836713697824806 37.0 37.0 37.0 22.0 37.0 46-47 34.082853923462395 37.0 37.0 37.0 24.5 37.0 48-49 34.565861118109794 37.0 37.0 37.0 29.5 37.0 50-51 34.83850536099213 37.0 37.0 37.0 32.0 37.0 52-53 34.0115863217715 37.0 37.0 37.0 24.5 37.0 54-55 34.17458917723468 37.0 37.0 37.0 24.5 37.0 56-57 34.03656462585034 37.0 37.0 37.0 24.5 37.0 58-59 33.72274712354077 37.0 37.0 37.0 22.0 37.0 60-61 33.57758741356625 37.0 37.0 37.0 17.5 37.0 62-63 33.209806556367404 37.0 37.0 37.0 17.5 37.0 64-65 32.94760435038212 37.0 34.5 37.0 17.5 37.0 66-67 33.19682749643067 37.0 37.0 37.0 13.0 37.0 68-69 33.340038072842304 37.0 37.0 37.0 17.5 37.0 70-71 33.4173805324599 37.0 37.0 37.0 22.0 37.0 72-73 33.47483623078861 37.0 37.0 37.0 22.0 37.0 74-75 32.88150527700792 37.0 34.5 37.0 13.0 37.0 76 33.4473628957756 37.0 37.0 37.0 13.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 11101 1 0.43426966177948856 11101 2 0.43254495533362913 11101 3 0.310187978961352 11101 4 -1.4158799706103018 11101 5 -1.0276614549842016 11101 6 0.7146206249958524 11101 7 -0.6967932230637928 11101 8 0.10605773383286277 11101 9 0.49376367247524655 11101 10-11 -0.3745289794261879 11101 12-13 -0.20651205890328583 11101 14-15 -0.5553108568043328 11101 16-17 -1.001986778486419 11101 18-19 0.6167809630513759 11101 20-21 -0.5208512586189826 11101 22-23 -0.42906353549405907 11101 24-25 0.7065702005549852 11101 26-27 -0.013250786320398333 11101 28-29 -0.3729796531901002 11101 30-31 -0.3030920001545496 11101 32-33 -0.42879227025021294 11101 34-35 -0.3131779670239041 11101 36-37 -0.6109505553492625 11101 38-39 -0.7495480388541012 11101 40-41 -0.0941047898618379 11101 42-43 -0.09238682955874111 11101 44-45 -0.5681819998428992 11101 46-47 -1.1343646909249756 11101 48-49 -0.39486079812929376 11101 50-51 -0.14395220632322037 11101 52-53 -0.7249871665495746 11101 54-55 -0.8192584291990528 11101 56-57 -1.0749897140819868 11101 58-59 -0.2604783640047259 11101 60-61 -0.7395472290858365 11101 62-63 -1.029691278668487 11101 64-65 -1.0191451652164147 11101 66-67 -0.028511948770969298 11101 68-69 0.34811240553267453 11101 70-71 -0.18100179389440996 11101 72-73 -0.976098743771054 11101 74-75 -0.375912116052163 11101 76 -0.6873311857844158 11102 1 -0.03759181008198098 11102 2 0.6181510159396879 11102 3 0.694387113160488 11102 4 0.1631243583940254 11102 5 0.766169713846967 11102 6 1.028473438848664 11102 7 1.279938378667815 11102 8 1.0422049199800512 11102 9 1.3839151876267621 11102 10-11 0.5245511071539042 11102 12-13 0.5878602354690088 11102 14-15 1.0209878444943712 11102 16-17 0.9295608405612015 11102 18-19 0.838642434912849 11102 20-21 0.9182721180043956 11102 22-23 0.0027546463241279184 11102 24-25 0.4197736637584484 11102 26-27 1.417755707186103 11102 28-29 0.4295095242990783 11102 30-31 0.5545919825294305 11102 32-33 0.6391189851610406 11102 34-35 1.5934778771319387 11102 36-37 0.2843416524429472 11102 38-39 0.05672901742295977 11102 40-41 0.2779190196619723 11102 42-43 0.44522139554948126 11102 44-45 -0.9115261556870564 11102 46-47 0.872128815568523 11102 48-49 0.04209807632958018 11102 50-51 0.5919785296075162 11102 52-53 0.39460157803917184 11102 54-55 0.9329060729654515 11102 56-57 0.6468717577794862 11102 58-59 -0.003714294740660762 11102 60-61 0.8895112124726055 11102 62-63 0.1713368598596574 11102 64-65 0.31121197764073116 11102 66-67 0.5480573152982942 11102 68-69 -0.014441707021440209 11102 70-71 0.563857513464896 11102 72-73 0.31367398350167264 11102 74-75 0.11651212637207209 11102 76 -0.10075109920433079 11103 1 0.05331728082710896 11103 2 -0.9945383779997066 11103 3 0.012568931342304523 11103 4 0.19721526748493545 11103 5 -0.2821257406984863 11103 6 -0.5785341369089068 11103 7 0.8433853483647837 11103 8 0.7012958290709577 11103 9 1.1395970058085823 11103 10-11 0.051066258669045794 11103 12-13 -0.594904916046147 11103 14-15 -0.2152810948995736 11103 16-17 -0.37773082610546993 11103 18-19 -0.5283083226629159 11103 20-21 -0.7351369729046979 11103 22-23 -0.5801052021607305 11103 24-25 0.4387130576978464 11103 26-27 0.12514207082246287 11103 28-29 -0.4786344150948594 11103 30-31 -0.7309193811069292 11103 32-33 -0.028494651202592536 11103 34-35 0.013458937738000998 11103 36-37 -0.20618865058736446 11103 38-39 -1.1156194674255318 11103 40-41 -0.1647893136713563 11103 42-43 0.4589524561555365 11103 44-45 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0.48662425930151443 11105 6 0.3193825297577604 11105 7 1.2857464594758952 11105 8 -0.032037504262376615 11105 9 -0.14512521641363918 11105 10-11 0.3468995920023801 11105 12-13 -0.16365491604614846 11105 14-15 -0.3350727615662379 11105 16-17 -0.15516138166102422 11105 18-19 0.41440001067042687 11105 20-21 0.2565296937619692 11105 22-23 0.4098253533948295 11105 24-25 0.402601946586735 11105 26-27 0.1911142930446914 11105 28-29 0.6123378071273606 11105 30-31 0.04373339667084508 11105 32-33 -0.24030020675814967 11105 34-35 0.5526950488491096 11105 36-37 0.3854780160793041 11105 38-39 0.060769421463362505 11105 40-41 -0.4901365358935763 11105 42-43 0.4988830117110936 11105 44-45 0.3643576826967845 11105 46-47 0.3862702297099432 11105 48-49 0.3904566621881642 11105 50-51 0.33819065081963373 11105 52-53 0.8698541032916935 11105 54-55 0.5839161739755525 11105 56-57 0.2948515557592799 11105 58-59 -0.12277995130632036 11105 60-61 -0.46970595924456404 11105 62-63 0.47070554672834675 11105 64-65 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1.1304741972383567 21611 1 -0.33432316861109257 21611 2 -0.09929025065888197 21611 3 0.5743666841512933 21611 4 0.7407545933276332 21611 5 -0.67292630249624 21611 6 0.1250005072858471 21611 7 1.10871774536853 21611 8 0.42039695266646504 21611 9 0.9928273428872316 21611 10-11 0.27461494780762763 21611 12-13 -0.05897326810606529 21611 14-15 -1.3781439226149246 21611 16-17 -0.15822005831520158 21611 18-19 -0.15957002678275956 21611 20-21 -0.6466538268372801 21611 22-23 0.1664420824834636 21611 24-25 0.23131605395251853 21611 26-27 -0.20245624378428317 21611 28-29 0.10902944258304359 21611 30-31 -0.4705612350395114 21611 32-33 -0.13418285344978642 21611 34-35 -0.07882804728072301 21611 36-37 -0.07182535470720808 21611 38-39 -0.5002168457026812 21611 40-41 -0.023345025281848564 21611 42-43 -0.8450620569530756 21611 44-45 0.652122976080058 21611 46-47 0.4523126766512675 21611 48-49 -0.1834509532924784 21611 50-51 -0.4464535439369186 21611 52-53 0.10399892226796936 21611 54-55 0.18622578695932646 21611 56-57 0.25259188035354185 21611 58-59 -0.11166884019520751 21611 60-61 0.5477721930650503 21611 62-63 0.29742215097303415 21611 64-65 0.5619142248317459 21611 66-67 0.2767983776067737 21611 68-69 0.5783826034994988 21611 70-71 0.3070265635159686 21611 72-73 0.3455304697120951 21611 74-75 0.08944368919127754 21611 76 -0.48481647203374933 21612 1 -0.003383750100727667 21612 2 -0.16195676287943073 21612 3 -0.16268880061645774 21612 4 0.015514236557098826 21612 5 -0.354612854100548 21612 6 0.9183516019227085 21612 7 -0.3916876756901999 21612 8 0.9280999527822971 21612 9 0.7111176243652864 21612 10-11 0.5946659150264395 21612 12-13 -0.5370226136406444 21612 14-15 -0.29039922204733415 21612 16-17 -0.011805138820243144 21612 18-19 0.46165086977695324 21612 20-21 0.38213106833241284 21612 22-23 0.3028952273925398 21612 24-25 0.43570618484560697 21612 26-27 -0.6625512281466115 21612 28-29 -0.14476414018077577 21612 30-31 -0.5500237625502251 21612 32-33 0.2585543178695744 21612 34-35 0.22216813017786308 21612 36-37 -0.27534672618872946 21612 38-39 -0.42897857395473693 21612 40-41 0.22830561760012102 21612 42-43 0.14092195787375061 21612 44-45 0.5676795612763925 21612 46-47 -1.0327446614698985 21612 48-49 -0.14001298271446672 21612 50-51 -0.3542492117233138 21612 52-53 0.5618930494543477 21612 54-55 -0.30514453621917426 21612 56-57 -0.4595012506553786 21612 58-59 -0.09105028349417665 21612 60-61 -0.2829934735630104 21612 62-63 -0.04521656094634352 21612 64-65 0.22165012197062595 21612 66-67 0.11735206693840894 21612 68-69 -0.1448306479773862 21612 70-71 1.1388926587319972 21612 72-73 -0.052129671606103045 21612 74-75 0.3311325574873507 21612 76 0.7483585540290392 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 0.0 10 6.0 11 19.0 12 60.0 13 136.0 14 233.0 15 428.0 16 703.0 17 1001.0 18 1261.0 19 1192.0 20 1249.0 21 1267.0 22 1340.0 23 1574.0 24 1687.0 25 1986.0 26 2156.0 27 2372.0 28 2633.0 29 3055.0 30 3515.0 31 4415.0 32 5660.0 33 7869.0 34 13258.0 35 25289.0 36 58519.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 73.35390946502058 5.514963186920859 4.028442652781277 17.102684695277286 2 68.11469443744575 5.513563450071387 20.137314184933235 6.23442792754962 3 68.07480193723579 6.116850032193947 20.18350550096582 5.624842529604434 4 68.31886898096305 14.692049272116462 9.703247480403135 7.285834266517358 5 67.48551272360797 9.510512023739537 19.39405391786344 3.6099213347890595 6 71.82091140055572 18.90410767152626 5.137214006257042 4.137766921660986 7 70.34685964642152 8.801668509679315 5.539536120714995 15.311935723184167 8 70.67110852935619 18.758704674453924 5.242751062057768 5.327435734132123 9 69.64600655076846 5.645838582346519 4.398673049466701 20.309481817418327 10-11 70.96945774194452 11.609767363735617 11.583172363595644 5.837602530724224 12-13 70.19039987122376 6.469606357627018 11.900254403062634 11.439739368086588 14-15 76.80934750810103 10.515246740339999 5.899232238964747 6.77617351259422 16-17 76.09311082105351 7.023554154258539 5.4911868760213745 11.392148148666571 18-19 70.31771083030344 6.19289411155398 11.071412324198725 12.417982733943862 20-21 76.25176724198268 6.144052968266633 6.469155503296518 11.135024286454177 22-23 76.35514869016873 5.909113178099258 5.119295077722021 12.616443054009979 24-25 71.66128338016733 11.704186365529235 5.424813043067458 11.209717211235963 26-27 76.6646019897886 11.309530058545837 5.428700408386088 6.597167543279476 28-29 75.96129619260918 7.31452967525196 10.996290593505039 5.727883538633819 30-31 71.90799304313745 11.438579792203974 5.975972928426202 10.677454236232375 32-33 75.84477666260726 7.431165049902715 5.7787063088789035 10.945351978611123 34-35 71.99512879644178 16.346177399995103 6.1764901438614785 5.482203659701638 36-37 76.30117475792709 7.344599158037661 11.233163377787731 5.121062706247528 38-39 76.79984322563261 6.005018179527647 12.095772341222212 5.099366253617534 40-41 76.37283034714446 11.124020156774916 7.197648376259798 5.305501119820829 42-43 71.73667321064254 11.315129182282972 11.400515819274283 5.547681787800209 44-45 71.11252642040286 6.010554163575538 12.099844627034896 10.777074788986717 46-47 75.86854887249619 5.504542209655519 7.5079436178105015 11.118965300037793 48-49 77.2452889612094 5.3921928857627766 11.78030899515336 5.582209157874477 50-51 81.72652958385241 5.4524012821769015 7.204896348035442 5.616172785935247 52-53 71.67913046216944 5.800301650680114 11.570508221905719 10.950059665244732 54-55 75.98246092902485 5.833175859631442 6.667086596538378 11.517276614805327 56-57 75.66821340836081 6.142874140018617 6.411629257913928 11.777283193706651 58-59 71.33273609137802 11.108350422385376 11.437999986002142 6.120913500234464 60-61 70.25125979843226 6.875349944008959 12.238591825307951 10.63479843225084 62-63 70.30350537687087 11.827016282837755 11.34339535485948 6.526082985431882 64-65 70.36538037563385 12.028051806254965 12.037850341373964 5.568717476737228 66-67 70.33398889969834 18.149273161206338 5.7576690766312755 5.759068862464043 68-69 70.4595798586931 18.558865625470236 5.216247143592023 5.765307372244638 70-71 70.77117431122029 18.25349332829412 5.3184676598112395 5.656864700674345 72-73 71.54445369858412 17.484024944183542 5.246747247671106 5.724774109561237 74-75 75.61660997494366 13.728495639636613 5.1162530270580495 5.538641358361679 76 83.01161814109741 6.443169092945129 4.98040313549832 5.564809630459126 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 1.5 7 1.5 8 0.0 9 1.5 10 2.5 11 3.0 12 4.0 13 4.5 14 6.5 15 7.0 16 21.0 17 36.0 18 36.0 19 42.5 20 57.0 21 65.0 22 92.5 23 120.5 24 142.5 25 164.0 26 198.5 27 288.5 28 353.5 29 363.0 30 431.0 31 556.0 32 647.5 33 682.0 34 713.5 35 810.0 36 988.0 37 1101.0 38 1150.0 39 1211.0 40 1358.0 41 1534.0 42 1575.0 43 2034.0 44 4826.5 45 8565.5 46 9971.0 47 6106.5 48 2105.0 49 1684.0 50 1400.0 51 1330.5 52 1211.5 53 1083.5 54 1005.0 55 1001.0 56 971.5 57 862.0 58 778.0 59 766.0 60 698.5 61 563.5 62 484.0 63 458.5 64 409.5 65 411.0 66 408.0 67 380.0 68 342.5 69 308.0 70 273.5 71 236.0 72 227.0 73 213.0 74 206.5 75 205.0 76 205.5 77 184.0 78 171.5 79 181.0 80 182.0 81 187.0 82 190.0 83 189.0 84 197.5 85 236.0 86 261.0 87 256.0 88 287.0 89 332.0 90 359.5 91 443.5 92 514.0 93 547.5 94 658.5 95 842.5 96 949.0 97 1213.0 98 2294.5 99 43900.5 100 84689.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0027994736989445986 5 0.0 6 0.0034993421236807483 7 0.0013997368494722993 8 6.998684247361497E-4 9 0.0 10-11 0.0 12-13 3.4993421236807483E-4 14-15 6.998684247361497E-4 16-17 3.4993421236807483E-4 18-19 0.0017496710618403741 20-21 0.004199210548416897 22-23 0.0020996052742084485 24-25 0.0024495394865765236 26-27 0.0031494079113126737 28-29 0.0027994736989445986 30-31 0.0024495394865765236 32-33 0.004199210548416897 34-35 0.0024495394865765236 36-37 0.0024495394865765236 38-39 0.0024495394865765236 40-41 0.0027994736989445986 42-43 0.0031494079113126737 44-45 0.0013997368494722993 46-47 0.0013997368494722993 48-49 0.0010498026371042243 50-51 0.0013997368494722993 52-53 0.0024495394865765236 54-55 0.0020996052742084485 56-57 0.0020996052742084485 58-59 0.0034993421236807483 60-61 0.0027994736989445986 62-63 0.0024495394865765236 64-65 0.003849276336048823 66-67 0.0034993421236807483 68-69 0.0024495394865765236 70-71 0.0031494079113126737 72-73 0.0034993421236807483 74-75 0.004199210548416897 76 0.0027994736989445986 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 142884.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 18.110495226897346 #Duplication Level Percentage of deduplicated Percentage of total 1 76.72063995053522 13.894487836286778 2 8.068941531089385 2.922650541698161 3 5.506820728832554 2.99193751574704 4 3.7639602736020406 2.726687382772039 5 2.3959500714920585 2.1695921166820638 6 1.441434478494416 1.5663055345595027 7 0.7612938130386058 0.9651185577111502 8 0.4057657379139777 0.5878894767783657 9 0.18935734435985624 0.30864197530864196 >10 0.6337674382656413 2.1408975112678816 >50 0.0579665339877111 0.7775538198818622 >100 0.0386443559918074 1.3675429019344365 >500 0.007728871198361479 1.099493295260491 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.007728871198361479 66.48120153411159 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 83427 58.387923070462755 No Hit CTTATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGGTG 11564 8.093278463648835 No Hit TCTTATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGGT 859 0.6011869768483525 No Hit ATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGGTGGTC 712 0.4983063184121385 No Hit CTCTTATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGG 436 0.3051426331849612 No Hit TCTTTATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGG 337 0.2358556591360824 No Hit GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 302 0.2113602642703172 No Hit GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 168 0.11757789535567313 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0027994736989445986 0.0 2 0.0 0.0 0.0 0.003499342123680748 0.0 3 0.0 0.0 0.0 0.003499342123680748 0.0 4 0.0 0.0 0.0 0.008398421096833796 0.0 5 0.0 0.0 0.0 0.01889644746787604 0.0 6 0.0 0.0 0.0 0.023095658016292937 0.0 7 0.0 0.0 0.0 0.026595000139973686 0.0 8 0.0 0.0 0.0 0.026595000139973686 0.0 9 0.0 0.0 0.0 0.026595000139973686 0.0 10 0.0 0.0 0.0 0.026595000139973686 0.0 11 0.0 0.0 0.0 0.026595000139973686 0.0 12 0.0 0.0 0.0 0.030794210688390583 0.0 13 0.0 0.0 0.0 0.03149407911312673 0.0 14 0.0 0.0 0.0 0.03639315808627978 0.0 15 0.0 0.0 0.0 0.03639315808627978 0.0 16 0.0 0.0 0.0 0.03639315808627978 0.0 17 0.0 0.0 0.0 0.03639315808627978 0.0 18 0.0 0.0 0.0 0.039892500209960526 0.0 19 0.0 0.0 0.0 0.05668934240362812 0.0 20 0.0 0.0 0.0 0.06298815822625346 0.0 21 0.0 0.0 0.0 0.07348618459729571 0.0 22 0.0 0.0 0.0 0.10218079001147784 0.0 23 0.0 0.0 0.0 0.10917947425883934 0.0 24 0.0 0.0 0.0 0.12247697432882618 0.0 25 0.0 0.0 0.0 0.13297500069986842 0.0 26 0.0 0.0 0.0 0.1385739480977576 0.0 27 0.0 0.0 0.0 0.14277315864617451 0.0 28 0.0 0.0 0.0 0.15397105344195292 0.0 29 0.0 0.0 0.0 0.17426723775930125 0.0 30 0.0 0.0 0.0 0.19946250104980265 0.0 31 0.0 0.0 0.0 0.21066039584558102 0.0 32 0.0 0.0 0.0 0.2456538170823885 0.0 33 0.0 0.0 0.0 0.28764592256655747 0.0 34 0.0 0.0 0.0 0.33663671229808795 0.0 35 0.0 0.0 0.0 0.3912264494275076 0.0 36 0.0 0.0 0.0 0.49480697628845777 0.0 37 0.0 0.0 0.0 0.6389798717841045 0.0 38 0.0 0.0 0.0 0.8041488200218359 0.0 39 0.0 0.0 0.0 1.0162089527168892 0.0 40 0.0 0.0 0.0 1.2086727695193304 0.0 41 0.0 0.0 0.0 1.4529268497522465 0.0 42 0.0 0.0 0.0 1.6502897455278407 0.0 43 0.0 0.0 0.0 1.885445536239187 0.0 44 0.0 0.0 0.0 2.151395537638924 0.0 45 0.0 0.0 0.0 2.4306430391086478 0.0 46 0.0 0.0 0.0 2.7756781725035693 0.0 47 0.0 0.0 0.0 3.011533831639652 0.0 48 0.0 0.0 0.0 3.2676856750930825 0.0 49 0.0 0.0 0.0 3.447551860250273 0.0 50 0.0 0.0 0.0 3.642815150751659 0.0 51 0.0 0.0 0.0 3.8429775202261975 0.0 52 0.0 0.0 0.0 4.037540942302847 0.0 53 0.0 0.0 0.0 4.263598443492623 0.0 54 0.0 0.0 0.0 4.4546625234455925 0.0 55 0.0 0.0 0.0 4.636628313876991 0.0 56 0.0 0.0 0.0 4.9172755521961875 0.0 57 0.0 0.0 0.0 5.123736737493351 0.0 58 0.0 0.0 0.0 5.324598975392626 0.0 59 0.0 0.0 0.0 5.49116766047983 0.0 60 0.0 0.0 0.0 5.626942134878643 0.0 61 0.0 0.0 0.0 5.799809635788471 0.0 62 0.0 0.0 0.0 5.9467820049830635 0.0 63 0.0 0.0 0.0 6.126648190140253 0.0 64 0.0 0.0 0.0 6.284118585705888 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTGTGC 30 1.0178701E-7 70.0 1 CGGGTGT 20 7.90491E-5 70.0 7 CGTGGGC 15 0.0022209072 70.0 1 TTGTGCG 30 1.0178701E-7 70.0 2 GCTGCGC 15 0.0022209072 70.0 8 CCATGCA 15 0.0022209072 70.0 9 TGCGCTA 15 0.0022209072 70.0 10 GTAGCAC 15 0.0022209072 70.0 55 CGTGTGC 15 0.0022209072 70.0 1 TCTTTAT 50 0.0 70.0 1 TAAGAAC 15 0.0022209072 70.0 21 GTTATAC 15 0.0022209072 70.0 1 CCATTGA 15 0.0022209072 70.0 48 TGTGCGC 40 1.058288E-8 61.25 3 CGCTGGC 35 2.957422E-7 60.0 16 TGCGGGC 30 8.34274E-6 58.333332 19 CGTCTGG 55 3.45608E-11 57.272724 9 CTTATGC 25 2.3839314E-4 55.999996 1 CGGTGGA 25 2.3839314E-4 55.999996 47 AAAAAGG 1040 0.0 55.528847 70 >>END_MODULE