FastQCFastQC Report
Wed 25 May 2016
SRR1780124_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780124_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142884
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGT5519238.62713809803757No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1724712.070630721424372No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCG77275.4078833179362285No Hit
ATTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGT68684.806696341087875No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCC27231.9057417205565352No Hit
ATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGTCTT14391.0071106631953193TruSeq Adapter, Index 8 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCC13330.9329246101732875No Hit
CTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGTC7190.5032053973852916No Hit
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGT5270.3688306598359508No Hit
CTTTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCG4010.28064723831919597No Hit
CTTTACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGTC3860.2701492119481537No Hit
CTTATACACATCTCCGAGCCCACGAGACAGAATTAAATCTCGTATGCCGT3260.22815710646398477No Hit
ATCTTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCC3080.21555947481873408No Hit
CTTATACACATCTCCGAGCCCACGAGACAGATTTAATCTCGTATGCCGTC3080.21555947481873408No Hit
CTTATACACATCTCCGAGCCCACGAGACAGATTTAACTCTCGTATGCCGT2920.2043615800229557No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCC2870.20086223789927493No Hit
CCTTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCG2680.1875647378292881No Hit
TATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGTCT2640.1847652641303435No Hit
TTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGTC2580.1805660535819266No Hit
CTTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGACGT2490.17426723775930125No Hit
CTTAATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCG2480.1735673693345651No Hit
CTAATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGT2220.15537079029142523No Hit
CTTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCAGT2150.15047171131827217No Hit
CTTATACACATCTCCGAGACCACGAGACAGATTTAAATCTCGTATGCCGT2130.14907197446879988No Hit
CTTATACACATCTCCGAGCCCACGAGACAGATTAAAATCTCGTATGCCGT2100.14697236919459142No Hit
ATTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGACGT1720.12037736905461772No Hit
CTTATACACATCTCCGAGCCCACGAGACAGATTTAAATATCGTATGCCGT1680.11757789535567313No Hit
CTTATACACATCTCCGAGCCAACGAGACAGATTTAAATCTCGTATGCCGT1530.1070798689846309No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGATTTAACTCTCGTATGCCG1510.1056801321351586No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTCG207.90491E-570.029
CTTCGAC150.002220907270.016
AACCGTA150.002220907270.05
CGGCGAT150.002220907270.013
ATCTAAG150.002220907270.010
CTCCGCC150.002220907270.08
TTTACCC150.002220907270.042
TAGACAG207.90491E-570.024
TAGAACT150.002220907270.04
TCTTAAT600.070.01
CGTCTTA150.002220907270.048
TGACGAC353.6834535E-970.045
CACCTAG150.002220907270.012
GTGCTAC150.002220907270.010
TACTGAT150.002220907270.052
TCTAAGA150.002220907270.011
CGAATGA150.002220907270.041
GTATCGG150.002220907270.09
TCATCCT207.90491E-570.027
ACCGTAT150.002220907270.06
Produced by FastQC (version 0.11.5)