FastQCFastQC Report
Wed 25 May 2016
SRR1780123_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780123_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78160
Sequences flagged as poor quality0
Sequence length76
%GC86

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5074564.92451381780963No Hit
CTTATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGGTG29003.7103377686796315No Hit
TCTTATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGGT2570.3288126919140225No Hit
ATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGGTGGTC1870.23925281473899693No Hit
CTCTTATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGG1470.18807574206755373No Hit
TCTTTATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGG860.11003070624360287No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG800.1023541453428864No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG790.10107471852610031No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGACG150.002217132470.011
CCCGGCG150.002217132470.024
GAAGTGA150.002217132470.033
GTATCAG207.882543E-570.057
TGTAGAA150.002217132470.037
AGGTCGA207.882543E-570.046
AAGTGAA150.002217132470.034
CGAAGAG401.0499207E-861.2532
AGTAGAT308.307545E-658.33333237
AAGAGTA308.307545E-658.33333234
GAGTAGA308.307545E-658.33333236
TGGTCGC5300.056.79245449
GTATCAT5150.056.40776457
CCGTATC5150.056.40776455
CATTAAA5150.056.40776461
CGTATCA5150.056.40776456
GTGGTCG5400.056.38889348
GGTCGCC5100.056.2745150
TCGCCGT5050.056.13861552
TATCATT5050.056.13861558