FastQCFastQC Report
Wed 25 May 2016
SRR1780123_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780123_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78160
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGT2473931.651740020470832No Hit
ATTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGT63508.124360286591607No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG52276.687563971340839No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCG52156.672210849539406No Hit
ATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGTCTT11001.4073694984646878TruSeq Adapter, Index 18 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCC10741.3741044012282497No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCC10411.3318833162743091No Hit
CTTATACACATCTCCGAGCCCACGAGACACGGGCGCATCTCGTATGCCGT8371.0708802456499487No Hit
CTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGTC4430.5667860798362334No Hit
CTTATACACATCTCCGAGCCCACGAGACCCGGGAGCATCTCGTATGCCGT3130.40046059365404296No Hit
CTTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCG2930.3748720573183214No Hit
ATCTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCC2740.35056294779938585No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCC2430.3109007164790174No Hit
ATTATACACATCTCCGAGCCCACGAGACACGGGCGCATCTCGTATGCCGT2320.2968270214943705No Hit
TTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGTC2180.2789150460593654No Hit
CTTTACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGTC2170.27763561924257935No Hit
TCTTATACACATCTCCGAGCCCACGAGCCCCGGGCGCATCTCGTATGCCG2130.272517911975435No Hit
CTTATACACATCTCCGAGCCCACGAGCCCCGGGCGCATCTCGTATGCCGT2120.2712384851586489No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGGGCGCATCTCGTATGCCGT1980.2533265097236438No Hit
TATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGTCT1950.24948822927328557No Hit
TCTTATACACATCTCCGCGCCCACGAGACCCGGGCGCATCTCGTATGCCG1790.2290174002047083No Hit
CTTAATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCG1730.2213408393039918No Hit
CTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGACGT1680.21494370522006143No Hit
TCTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGT1400.17911975435005117No Hit
ATTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGACGT1340.1714431934493347No Hit
CTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGA1100.14073694984646878No Hit
CTTATACACATCTCCGCGCCCACGAGACCCGGGCGCATCTCGTATGCCGT1060.13561924257932445No Hit
CTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTAAGCCGT1050.1343398157625384No Hit
CTAATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGT1010.12922210849539406No Hit
CTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCAGT960.12282497441146367No Hit
ATATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGTC950.12154554759467757No Hit
CTTATACACATCTCCGAGACCACGAGACCCGGGCGCATCTCGTATGCCGT930.11898669396110542No Hit
TCTTTATACACATCTCCGAGCCCACGAGCCCCGGGCGCATCTCGTATGCC890.1138689866939611No Hit
CCTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCG880.11258955987717503No Hit
ATTATACACATCTCCGAGACCACGAGACCCGGGCGCATCTCGTATGCCGT830.10619242579324463No Hit
CTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGAATGCCGT830.10619242579324463No Hit
TCTTATACACATCTCCGAGCCCACGAGACACGGGCGCATCTCGTATGCCG820.10491299897645853No Hit
ATTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCAGT810.10363357215967246No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGCG207.860057E-570.0403327
TTCCTCT150.002212067670.0403328
GACAAGG207.860057E-570.0403326
CAAGGGC207.860057E-570.0403328
CCGCGCG150.002212067670.0403329
TCCTTGA150.002217694769.9955355
AACAACT150.002217694769.995537
TGCAAGA150.002217694769.9955355
TCTGCAA150.002217694769.9955353
AGAGCAT150.002217694769.9955332
GACGTAT150.002217694769.9955346
GACAACG150.002217694769.9955318
GAACACG150.002217694769.9955318
TAATAAA150.002217694769.995532
GAGAGCA150.002217694769.9955331
GTAATCT150.002217694769.9955349
CGTCCTG150.002217694769.9955339
CTCAACT150.002217694769.9955334
ACAACGA150.002217694769.9955319
CCTGGGT150.002217694769.995537
Produced by FastQC (version 0.11.5)