FastQCFastQC Report
Wed 25 May 2016
SRR1780122_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780122_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences253373
Sequences flagged as poor quality0
Sequence length76
%GC67

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7166428.283992374878142No Hit
CTTATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGGTG48911.9303556416824206No Hit
TCTTATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGGT4220.16655286869555952No Hit
CTCTTATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGG3120.12313861382230941No Hit
ATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGGTGGTC3030.11958653842358893No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCAAC150.002221331570.01243615
GGGAACC150.002223071469.9986212
CGCTAAG150.002223071469.9986212
TACCCGT200.006946356552.49896236
GAGTATT200.006946356552.49896221
AGCGCTA200.006946356552.49896210
GTGAATC200.006946356552.4989624
GAGGGAC200.006946356552.49896252
GGCCCTA352.08307E-549.9990121
GTATCAT7850.049.04361357
TAGATCT7700.048.63540339
GTAGATC8000.048.5615438
GCCGTAT7900.048.29018854
AGATCTC7900.047.84715740
CGGTGGT8200.047.80393646
CTCGGTG8000.047.68655844
CGTATCA8100.047.52992656
ATCATTA8100.047.52992659
TCATTAA8250.047.08997760
ATATCGC305.871252E-446.66574525
Produced by FastQC (version 0.11.5)