FastQCFastQC Report
Wed 25 May 2016
SRR1780122_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780122_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences253373
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCGT4148816.37427823801273No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG78983.117143499899358No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCG55232.1797902696814577No Hit
ATTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCGT45921.812347803436041No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCC26641.0514143180212572No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTAGTATGCCGT15030.5931965915863174No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCC10480.4136194464287829No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGACGT8380.3307376871253054No Hit
ATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCGTCTT8270.32639626163798036RNA PCR Primer, Index 26 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTAAGCCGT6770.2671950049926393No Hit
CTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCGTC4890.19299609666381187No Hit
ATTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTAGTATGCCGT4240.16734221878416405No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCGA3660.1444510662146322No Hit
CTTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCG3310.1306374396640526No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTAGTATGCCG3070.12116523860079803No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCCT3060.12077056355649575No Hit
ATCTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCC2990.11800783824637985No Hit
CTTTACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCGTC2880.11366641275905483No Hit
ATTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGACGT2770.10932498727172983No Hit
ATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCGTCAT2610.10301018656289344No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTCTA150.002222424870.003751
TTGTCTA150.002224165569.9899440
TACGGGT150.002224165569.989947
AGGGGGG49650.063.79999570
ATTATAC8050.061.7424351
CTCGTAA1900.057.09705439
TCGTAAG1600.056.86682540
TAATCCG252.3891653E-455.9919543
CTTAAGC1950.055.6440151
TCTTTAT2150.055.3518031
TCTTAAG2100.055.0029550
CCGACTT900.054.4366247
GTCTTCA5800.054.31325549
GTCTTAA1950.053.8490449
TTAAGCA850.053.5322852
TCGAATG850.053.52171340
GCCGTCT52000.053.5019246
CCGTCTT52450.053.24305747
CGTAAGC1650.053.0226841
CGTCTTC47000.052.94964648
Produced by FastQC (version 0.11.5)