FastQCFastQC Report
Wed 25 May 2016
SRR1780121_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780121_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111201
Sequences flagged as poor quality0
Sequence length76
%GC81

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6538658.799830936772146No Hit
CTTATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGGTG42493.821008803877663No Hit
TCTTATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGGT3520.3165439159719787No Hit
ATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGGTGGTC2580.23201230204764348No Hit
CTCTTATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGG1940.17445886278001096No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1350.12140178595516228No Hit
TCTTTATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGG1310.11780469600093525No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAG150.002219608870.000011
TTGGCCG150.002219608870.0000122
GAGTCTT150.002219608870.0000117
GGCCGCA150.002219608870.0000124
GCCGCAT150.002219608870.0000125
GGAGTCT150.002219608870.0000116
CGTGTGC150.002219608870.000011
ACGTAGC150.002219608870.000012
TTTTGAC150.002219608870.0000132
AGGTGGT150.002219608870.0000146
CCGCATG150.002219608870.0000126
TGCGGGC207.897216E-570.019
CTGCGGG252.824143E-670.018
TGCGCGT453.8016879E-1062.2222185
GCGCGTC401.05537765E-861.256
CGCGTCT401.05537765E-861.257
GCGCTGG401.05537765E-861.2515
GTGCGCG401.05537765E-861.254
GTATCAA352.9514013E-760.057
CGCTGCG308.330626E-658.33333616
Produced by FastQC (version 0.11.5)