FastQCFastQC Report
Wed 25 May 2016
SRR1780121_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780121_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111201
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGT4022636.174135124684135No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG68966.201383081087401No Hit
ATTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGT63285.690596307587162No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCG60905.476569455310654No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCC18701.6816395536011366No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCC12001.079126986268109No Hit
ATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGTCTT10680.9604230177786172RNA PCR Primer, Index 39 (95% over 22bp)
CTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGTC5830.5242758608285897No Hit
CTTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCG3220.2895657413152759No Hit
ATCTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCC3130.2814722889182651No Hit
CTTTACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGTC3050.27427810900981103No Hit
CTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGACGT2960.2661846566128002No Hit
CTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTAAGCCGT2790.25089702430733535No Hit
CTTATACACATCTCCGAGCCCACGAGACGCAAGTACATCTCGTATGCCGT2740.2464006618645516No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCC2590.23291157453620023No Hit
TTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGTC2520.22661666711630293No Hit
CTTAATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCG2160.19424285752825965No Hit
TATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGTCT2150.1933435850397029RNA PCR Primer, Index 39 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGCATGTACATCACGTATGCCGT1870.16816395536011366No Hit
CTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTAGTATGCCGT1860.1672646828715569No Hit
CCTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCG1830.16456686540588664No Hit
ATTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGACGT1620.1456821431461947No Hit
CTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCAGT1540.13848796323774065No Hit
CTTATACACATCTCCGAGCCCACGAGACGCATGAACATCTCGTATGCCGT1490.13399160079495687No Hit
CTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGA1450.13039451084072984No Hit
TCTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGT1320.11870396848949201No Hit
CTAATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGT1170.10521488116114062No Hit
ATTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTAAGCCGT1160.10431560867258387No Hit
ATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGTCAT1130.1016177912069136No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGACT150.002213445570.0490626
CGAGACT150.002213445570.0490623
ATCCCAC150.002217401370.0175517
TCCCACG150.002217401370.0175518
TCCGGAA150.002221362469.9860652
CTATGCC301.01719706E-769.9860642
AGACTCC150.002221362469.9860614
TCCGATC150.002221362469.9860613
CCGATCC150.002221362469.9860614
CGGCCAA150.002221362469.986068
GATCCCA150.002221362469.9860616
TGCGGCC150.002221362469.986066
TGCATGC150.002221362469.986062
AGCGGGG207.905008E-569.9860670
AACGAGC150.002221362469.9860613
CCGACTT401.3278623E-1069.9860647
CCGACAT207.905008E-569.9860647
ATGCGGC150.002221362469.986065
AACTCGT252.8274844E-669.9860637
ACGTAAT150.002221362469.9860647
Produced by FastQC (version 0.11.5)