FastQCFastQC Report
Wed 25 May 2016
SRR1780120_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780120_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207052
Sequences flagged as poor quality0
Sequence length76
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7016433.88713946255047No Hit
CTTATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGGTG41912.0241292042578674No Hit
TCTTATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGGT3330.16082916368834882No Hit
CTCTTATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGG2960.14295925661186562No Hit
ATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGGTGGTC2850.1376465815350733No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATATC150.002221439570.0071
ACGTGGG150.002221439570.00727
GACGGCG150.002221439570.00723
TTACGTG150.002221439570.00725
CCACCCG150.002221439570.00726
CTAACGT150.00222356969.990116
CACCGGG150.00222356969.99019
GTCTGGA252.385265E-456.0056041
GCCGTAT7350.055.2436254
TGGTCGC7550.055.1577649
TCGCCGT7450.054.9586752
CGGTGGT7900.054.48596646
TCGGTGG7950.054.14328445
CCGTATC7500.054.13875255
CTCGGTG7700.054.08325244
GATCTCG7550.053.78021241
CGCCGTA7700.053.64172753
GTCGCCG7700.053.62877351
AGATCTC7650.053.53476740
TCTCGGT7800.053.40278243
Produced by FastQC (version 0.11.5)