FastQCFastQC Report
Wed 25 May 2016
SRR1780120_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780120_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207052
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGT4330120.91310395456214No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG68883.326700538995035No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCG62403.0137356799258157No Hit
ATTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGT59382.867878600544791No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCC24081.1629928713559878No Hit
ATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGTCTT14150.6834032030601008Illumina PCR Primer Index 3 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCC12090.5839112879856269No Hit
CTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGTC6170.29799277476189556No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCAGCCATCTCGTATGCCGT3680.1777331298417789No Hit
CTTTACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGTC3640.1758012479956726No Hit
ATCTTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCC3450.1666248092266677No Hit
CTTTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCG3130.15116975445781736No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGCCATCTCGTATGCCGT3030.1463400498425516No Hit
TATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGTCT2730.13185093599675443No Hit
CTTAATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCG2690.12991905415064817No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGACGT2640.12750420184301528No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCC2480.11977667445859011No Hit
TTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGTC2310.11156617661263837No Hit
CCTTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCG2260.1091513243050055No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGAAGACATCTCGTATGCCGT2090.10094082645905376No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCACGT150.002219949670.0188451
GACTCGG150.002219949670.01884526
GACGTCC150.002222078270.001937
ATTCAGT150.002222078270.0019344
ACGTCCG150.002222078270.001938
CCGGTCT150.002222078270.001939
GGCCGCT150.002224208369.9850233
CGGGAAT150.002224208369.9850213
AGGGGGG55800.063.4310570
ATTATAC9900.059.7635571
ATCGTAT900.058.33494639
TCCGAGA900.058.32085813
TCGAAAG252.3861187E-456.00154540
GACCACG1000.055.98802618
CGAAGAC1350.054.45910330
CACGTAT900.054.4459539
AGCTTGA2250.054.43279655
TGCCGTC71100.053.21525245
CCGTCTT69500.053.1813247
ATGCCGT71600.053.13694444
Produced by FastQC (version 0.11.5)