FastQCFastQC Report
Wed 25 May 2016
SRR1780119_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780119_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences271126
Sequences flagged as poor quality0
Sequence length76
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7314926.979706852164675No Hit
CTTATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGGTG48411.7855166970338514No Hit
TCTTATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGGT3960.1460575525770306No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATA207.921843E-569.9926255
CGTATAA150.002223995769.99261556
CACGCTT150.002223995769.99261544
GCTCGAA150.002223995769.9926151
GCCGTAT8700.053.09785554
CTCGGTG8700.052.69559544
CGAAGAG200.006939098252.5138432
CGCCAAC200.006944163752.5041563
CGAATCA200.00694923252.4944756
TGGTCGC8800.052.4944749
GTAGATC8950.052.40637638
CGGTGGT8950.052.39671346
TCGCCGT8850.052.19788752
TCGGTGG9000.052.10561845
TCTCGGT8800.052.09678343
CGTATCA8750.051.99452256
GTGGTCG9150.051.25142748
AGATCTC9050.051.04434240
GTATCAT8850.051.0209857
TAGATCT9000.050.93907539
Produced by FastQC (version 0.11.5)