FastQCFastQC Report
Wed 25 May 2016
SRR1780119_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780119_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences271126
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGT4309515.894823808856398No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG78522.896070461704152No Hit
ATTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGT72752.6832542802977213No Hit
TCTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCG66832.4649056158391303No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCC20740.764957990012024No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCC13560.5001364679152867No Hit
ATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGTCTT12730.46952339502666657TruSeq Adapter, Index 3 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACAACTAGGCATCTCGTATGCCGT5610.20691486615079338No Hit
CTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGTC5420.19990705428472372TruSeq Adapter, Index 3 (95% over 21bp)
ATCTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCC3650.13462375426923276No Hit
CTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGACGT3510.12946010342054984No Hit
CTTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCG3220.1187639695197067No Hit
CTTTACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGTC2840.1047483457875674RNA PCR Primer, Index 3 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTAAGCCGT2730.10069119154931655No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAA207.927325E-569.9828546
AGGGGGG54850.064.64793470
ATTATAC12900.060.489051
CAAATCG352.9724652E-759.98529436
GCCGTCA3550.058.1547646
TGCATGA4150.056.51302755
CCGTCAT3700.055.7971347
CGTCATC3650.054.66430748
ATCGTAT1350.054.43110339
CGTCTTC67600.053.95638748
CCGTCTT68050.053.88833647
TGCCGTC71750.053.84046645
ATGCCGT72350.053.82923544
ATCTCGT71800.053.70549837
TATGCCG72550.053.68084743
TCGTATG73050.053.64872440
GCCGTCT68750.053.64503546
CATCTCG72450.053.61004636
CGTATGC72550.053.58438541
TCTCGTA71850.053.5707338
Produced by FastQC (version 0.11.5)