FastQCFastQC Report
Wed 25 May 2016
SRR1780118_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780118_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165618
Sequences flagged as poor quality0
Sequence length76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5453632.928787933678706No Hit
CTTATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGGTG35162.122957649530848No Hit
TCTTATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGGT3040.18355492760448744No Hit
CTCTTATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGG1880.11351423154488038No Hit
ATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGGTGGTC1830.11049523602506973No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGGG150.00222153470.09
CGCCGAA150.00222153470.053
AAAAAGG4150.053.97590670
CGTATCA5950.052.94117456
CCGTATC5900.052.7966155
TTTCGTT200.006941586752.523
ACGGGCA200.006941586752.513
GCCGAAA200.006941586752.554
GGCGCGT200.006941586752.55
CGCCGTA6000.052.553
GCCGTAT6000.052.554
GTCGGGC200.006941586752.510
TCGCCGT6100.052.21311652
CGGTGGT6450.052.09302546
GGTCGCC6200.051.93548250
GTCGCCG6250.051.5251
TCGGTGG6600.051.43939245
TGGTCGC6350.051.25984249
GTATCAT6150.051.2195157
TCTCGGT6350.050.7086643
Produced by FastQC (version 0.11.5)