FastQCFastQC Report
Wed 25 May 2016
SRR1780118_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780118_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165618
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGT3072418.551123670132476No Hit
ATTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGT58103.5080727940199736No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG56623.4187105266335784No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCG53473.2285138088855074No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCC13710.8278085715320799No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCC10710.6466688403434409No Hit
ATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGTCTT9230.5573065729570458TruSeq Adapter, Index 11 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGTC4860.293446364525595No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCCAACATCTCGTATGCCGT3840.23185885592145783Illumina PCR Primer Index 6 (95% over 21bp)
ATCTTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCC2810.16966754821335844No Hit
CTTTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCG2550.15396877151034308No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCC2500.15094977599053241No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTAAGCCGT2420.14611938315883538No Hit
CTTTACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGTC2270.13706239659940345No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGACGT2100.12679781183204725No Hit
TATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGTCT2080.12559021362412298No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCACACATCTCGTATGCCGT1950.1177408252726153No Hit
CTTAATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCG1890.11411803064884252No Hit
TTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGTC1750.10566484319337269No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATATCG401.3460522E-1070.0181236
ATATCGT353.6798156E-970.0181237
TCGCAAA150.002221914269.9969829
GCCGAAA150.002221914269.9969846
CCGAAAT150.002221914269.9969847
CGCAAAC150.002221914269.9969830
AGGGGGG40200.064.7928970
TATCGTA501.4551915E-1163.0163138
AATGCCG501.4551915E-1162.99728443
ACTCGAC453.8198777E-1062.21954327
ATTATAC9550.061.9344981
ATCGTAT452.6791895E-854.4585439
CTCGTAA1100.054.1049139
TCGTAAG1100.054.1049140
TGCTTGA49700.052.744255
CTGCTTG48750.052.69516454
ATTGTGT200.00693448152.5135939
AACTCGA200.00693448152.5135937
TCACGTA608.367351E-1152.51358838
GGTAAGC200.006942770452.49773829
Produced by FastQC (version 0.11.5)