FastQCFastQC Report
Wed 25 May 2016
SRR1780117_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780117_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116534
Sequences flagged as poor quality0
Sequence length76
%GC72

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5121243.945972849125575No Hit
CTTATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGGTG29422.5245850996275765No Hit
TCTTATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGGT2440.20938095319820824No Hit
ATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGGTGGTC2040.1750562067722725No Hit
CTCTTATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGG1850.15875195221995297No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGTC150.00221987570.000016
GTTTAGG150.00221987570.0000159
AGGCGCA150.00221987570.0000114
GGGCGCT150.00221987570.0000113
CTTGTGC252.8249888E-670.01
TGTGCGC207.898796E-570.03
TGCGCGT207.898796E-570.05
TCTTTAT207.898796E-570.01
GGATTAC207.898796E-570.01
TCTGGCG308.333112E-658.33333611
GTCTGGC252.3820982E-455.99999610
ATTCATG252.3820982E-455.99999664
AACGAAG252.3820982E-455.99999630
CTTTATA252.3820982E-455.9999962
CGGTGGT5100.052.84313246
TCGGTGG5100.052.84313245
AGATCTC4650.052.68817540
CTCGGTG4850.052.68041244
TCTTGGG200.006936445452.563
AGATCTA200.006936445452.537
Produced by FastQC (version 0.11.5)