FastQCFastQC Report
Wed 25 May 2016
SRR1780117_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780117_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116534
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGT3013225.856831482657423No Hit
ATTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGT55294.744538074724973No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCG49074.2107882678016715No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG46914.025434637101618No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCC19801.699074948083821No Hit
ATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGTCTT10790.9259100348396175RNA PCR Primer, Index 30 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCC9400.8066315410094909No Hit
CTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGTC4240.36384231211491924No Hit
ATCTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCC3330.28575351399591536No Hit
CTTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCG2670.22911768239312133No Hit
CTTTACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGTC2510.21538778382274704No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCC2120.18192115605745962No Hit
TATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGTCT2080.17848868141486604No Hit
TTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGTC1860.15961007088060136No Hit
CTTAATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCG1660.1424476976676335No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGACGT1630.1398733416856883No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATATCGTATGCCGT1590.13644086704309472No Hit
CCTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCG1360.11670413784818164No Hit
CTTATACACATCTCCGAGCCCACGAGACGACTCTGGATCTCGTATGCCGT1260.10812295124169771No Hit
CTAATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGT1260.10812295124169771No Hit
ATTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGACGT1250.1072648325810493No Hit
ATTATACACATCTCCGAGCCCACGAGACGCCTCTGGATATCGTATGCCGT1220.10469047659910412No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCTCTGGCTCTCGTATGCCGT1170.10039988329586215No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACGA150.002220685869.993567
GTGGGAT150.002220685869.993563
GATCCTA150.002220685869.993561
GGATACG150.002220685869.993566
ACGATTC150.002220685869.9935616
TACGAAG150.002220685869.993569
GAAAACG150.002220685869.9935638
GGGATAC150.002220685869.993565
TGCCGTA207.902394E-569.9935645
AGGGGGG42600.064.3250770
ATTATAC8450.063.7811131
CGTCTAC401.0568328E-861.24436648
AAGCCGT401.0568328E-861.24436644
TGATTGA352.9545117E-759.99448455
CGTCTTA308.33766E-658.3279748
TCTTTAT1200.058.327971
AGACGAC553.45608E-1157.2920525
GAGACGA553.45608E-1157.2920524
ACGACTC509.658834E-1056.01889427
CGTATGA750.055.9948541
Produced by FastQC (version 0.11.5)