FastQCFastQC Report
Wed 25 May 2016
SRR1780116_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780116_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97552
Sequences flagged as poor quality0
Sequence length76
%GC91

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7383475.68681318681318No Hit
CTTATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGGTG58365.9824503854354605No Hit
TCTTATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGGT5090.5217730031162867No Hit
ATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGGTGGTC3140.32187961292438905No Hit
CTCTTATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGG2770.2839511235033623No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2350.2408971625389536No Hit
TCTTTATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGG1740.17836640970969328No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1050.1076349024110218No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1000.10250943086763983No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC150.00221878970.000016
TATGACG150.00221878970.0000111
TACACGT150.00221878970.000015
TTATAAA150.00221878970.000012
TCGCCGA150.00221878970.0000152
ATAAACA150.00221878970.000014
CGAATCA150.00221878970.0000156
CTTATAA150.00221878970.000011
GCGTCTG150.00221878970.000018
ATATGAC150.00221878970.0000110
CTTGTGC301.0144868E-770.000011
CGACGAG150.00221878970.0000122
GTGGCGC150.00221878970.0000112
AAAGTGT150.00221878970.0000133
ATACACG150.00221878970.000014
CAAGTGT150.00221878970.0000133
CGCCGAA150.00221878970.0000153
GTTGTGC150.00221878970.000011
CCGAATC150.00221878970.0000155
TCGGGGG150.00221878970.0000145
Produced by FastQC (version 0.11.5)